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Development of chloroplast microsatellite markers for the endangered Maianthemum bicolor (Asparagaceae s.l.)(1)

PREMISE OF THE STUDY: Ten polymorphic chloroplast microsatellite (cpSSR) markers were developed and characterized in an endemic and endangered herb, Maianthemum bicolor (Asparagaceae s.l.), for use in conservation genetics. METHODS AND RESULTS: Primer sets flanking each of the 10 cpSSR loci in nonco...

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Autores principales: Park, Hana, Kim, Changkyun, Lee, You-Mi, Kim, Joo-Hwan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Botanical Society of America 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5001858/
https://www.ncbi.nlm.nih.gov/pubmed/27610276
http://dx.doi.org/10.3732/apps.1600032
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author Park, Hana
Kim, Changkyun
Lee, You-Mi
Kim, Joo-Hwan
author_facet Park, Hana
Kim, Changkyun
Lee, You-Mi
Kim, Joo-Hwan
author_sort Park, Hana
collection PubMed
description PREMISE OF THE STUDY: Ten polymorphic chloroplast microsatellite (cpSSR) markers were developed and characterized in an endemic and endangered herb, Maianthemum bicolor (Asparagaceae s.l.), for use in conservation genetics. METHODS AND RESULTS: Primer sets flanking each of the 10 cpSSR loci in noncoding regions of the chloroplast genome of M. bicolor were designed. These cpSSR markers were tested on a total of 33 adult individuals from three natural populations in South Korea. The number of alleles per locus ranged from two to three. The unbiased haplotype diversity per locus ranged from 0.061 to 0.682. All markers were successfully transferred to the congeneric species M. japonicum, M. bifolium, and M. dilatatum with polymorphisms among the species. CONCLUSIONS: The developed cpSSR markers will be useful in assessing the genetic diversity and population structure of M. bicolor and will help to infer its molecular identification, thereby providing a basis for conservation.
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spelling pubmed-50018582016-09-08 Development of chloroplast microsatellite markers for the endangered Maianthemum bicolor (Asparagaceae s.l.)(1) Park, Hana Kim, Changkyun Lee, You-Mi Kim, Joo-Hwan Appl Plant Sci Primer Note PREMISE OF THE STUDY: Ten polymorphic chloroplast microsatellite (cpSSR) markers were developed and characterized in an endemic and endangered herb, Maianthemum bicolor (Asparagaceae s.l.), for use in conservation genetics. METHODS AND RESULTS: Primer sets flanking each of the 10 cpSSR loci in noncoding regions of the chloroplast genome of M. bicolor were designed. These cpSSR markers were tested on a total of 33 adult individuals from three natural populations in South Korea. The number of alleles per locus ranged from two to three. The unbiased haplotype diversity per locus ranged from 0.061 to 0.682. All markers were successfully transferred to the congeneric species M. japonicum, M. bifolium, and M. dilatatum with polymorphisms among the species. CONCLUSIONS: The developed cpSSR markers will be useful in assessing the genetic diversity and population structure of M. bicolor and will help to infer its molecular identification, thereby providing a basis for conservation. Botanical Society of America 2016-08-23 /pmc/articles/PMC5001858/ /pubmed/27610276 http://dx.doi.org/10.3732/apps.1600032 Text en © 2016 Park et al. Published by the Botanical Society of America http://creativecommons.org/licenses/by-nc/4.0/ This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA).
spellingShingle Primer Note
Park, Hana
Kim, Changkyun
Lee, You-Mi
Kim, Joo-Hwan
Development of chloroplast microsatellite markers for the endangered Maianthemum bicolor (Asparagaceae s.l.)(1)
title Development of chloroplast microsatellite markers for the endangered Maianthemum bicolor (Asparagaceae s.l.)(1)
title_full Development of chloroplast microsatellite markers for the endangered Maianthemum bicolor (Asparagaceae s.l.)(1)
title_fullStr Development of chloroplast microsatellite markers for the endangered Maianthemum bicolor (Asparagaceae s.l.)(1)
title_full_unstemmed Development of chloroplast microsatellite markers for the endangered Maianthemum bicolor (Asparagaceae s.l.)(1)
title_short Development of chloroplast microsatellite markers for the endangered Maianthemum bicolor (Asparagaceae s.l.)(1)
title_sort development of chloroplast microsatellite markers for the endangered maianthemum bicolor (asparagaceae s.l.)(1)
topic Primer Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5001858/
https://www.ncbi.nlm.nih.gov/pubmed/27610276
http://dx.doi.org/10.3732/apps.1600032
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