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Microsatellites in the tree Foetidia mauritiana (Lecythidaceae) and utility in other Foetidia taxa from the Mascarene Islands(1)

PREMISE OF THE STUDY: Polymorphic markers were required for a native tree of the Mascarene Islands, Foetidia mauritiana (Lecythidaceae), to investigate the effects of fragmentation of lowland tropical habitats on tree mating systems and on gene flow. METHODS AND RESULTS: Using microsatellite enrichm...

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Autores principales: Martos, Florent, Lebreton, Gérard, Rivière, Eric, Humeau, Laurence, Chevallier, Marie-Hélène
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Botanical Society of America 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5001860/
https://www.ncbi.nlm.nih.gov/pubmed/27610278
http://dx.doi.org/10.3732/apps.1600034
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author Martos, Florent
Lebreton, Gérard
Rivière, Eric
Humeau, Laurence
Chevallier, Marie-Hélène
author_facet Martos, Florent
Lebreton, Gérard
Rivière, Eric
Humeau, Laurence
Chevallier, Marie-Hélène
author_sort Martos, Florent
collection PubMed
description PREMISE OF THE STUDY: Polymorphic markers were required for a native tree of the Mascarene Islands, Foetidia mauritiana (Lecythidaceae), to investigate the effects of fragmentation of lowland tropical habitats on tree mating systems and on gene flow. METHODS AND RESULTS: Using microsatellite enrichment and next-generation sequencing, we identified 13 microsatellite loci (dinucleotide repeats). They were highly polymorphic in 121 trees sampled in the largest three populations on Réunion, revealing 2–17 different alleles per locus. Furthermore, they were found to be polymorphic in conspecific populations on Mauritius and in F. rodriguesiana from Rodrigues. CONCLUSIONS: These results indicate the utility of these markers to investigate genetic diversity, mating systems, and gene flow in a genus native to the biodiversity hotspot of Madagascar and the Indian Ocean islands.
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spelling pubmed-50018602016-09-08 Microsatellites in the tree Foetidia mauritiana (Lecythidaceae) and utility in other Foetidia taxa from the Mascarene Islands(1) Martos, Florent Lebreton, Gérard Rivière, Eric Humeau, Laurence Chevallier, Marie-Hélène Appl Plant Sci Primer Note PREMISE OF THE STUDY: Polymorphic markers were required for a native tree of the Mascarene Islands, Foetidia mauritiana (Lecythidaceae), to investigate the effects of fragmentation of lowland tropical habitats on tree mating systems and on gene flow. METHODS AND RESULTS: Using microsatellite enrichment and next-generation sequencing, we identified 13 microsatellite loci (dinucleotide repeats). They were highly polymorphic in 121 trees sampled in the largest three populations on Réunion, revealing 2–17 different alleles per locus. Furthermore, they were found to be polymorphic in conspecific populations on Mauritius and in F. rodriguesiana from Rodrigues. CONCLUSIONS: These results indicate the utility of these markers to investigate genetic diversity, mating systems, and gene flow in a genus native to the biodiversity hotspot of Madagascar and the Indian Ocean islands. Botanical Society of America 2016-08-19 /pmc/articles/PMC5001860/ /pubmed/27610278 http://dx.doi.org/10.3732/apps.1600034 Text en © 2016 Martos et al. Published by the Botanical Society of America http://creativecommons.org/licenses/by-nc/4.0/ This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA).
spellingShingle Primer Note
Martos, Florent
Lebreton, Gérard
Rivière, Eric
Humeau, Laurence
Chevallier, Marie-Hélène
Microsatellites in the tree Foetidia mauritiana (Lecythidaceae) and utility in other Foetidia taxa from the Mascarene Islands(1)
title Microsatellites in the tree Foetidia mauritiana (Lecythidaceae) and utility in other Foetidia taxa from the Mascarene Islands(1)
title_full Microsatellites in the tree Foetidia mauritiana (Lecythidaceae) and utility in other Foetidia taxa from the Mascarene Islands(1)
title_fullStr Microsatellites in the tree Foetidia mauritiana (Lecythidaceae) and utility in other Foetidia taxa from the Mascarene Islands(1)
title_full_unstemmed Microsatellites in the tree Foetidia mauritiana (Lecythidaceae) and utility in other Foetidia taxa from the Mascarene Islands(1)
title_short Microsatellites in the tree Foetidia mauritiana (Lecythidaceae) and utility in other Foetidia taxa from the Mascarene Islands(1)
title_sort microsatellites in the tree foetidia mauritiana (lecythidaceae) and utility in other foetidia taxa from the mascarene islands(1)
topic Primer Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5001860/
https://www.ncbi.nlm.nih.gov/pubmed/27610278
http://dx.doi.org/10.3732/apps.1600034
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