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Microsatellites in the tree Foetidia mauritiana (Lecythidaceae) and utility in other Foetidia taxa from the Mascarene Islands(1)
PREMISE OF THE STUDY: Polymorphic markers were required for a native tree of the Mascarene Islands, Foetidia mauritiana (Lecythidaceae), to investigate the effects of fragmentation of lowland tropical habitats on tree mating systems and on gene flow. METHODS AND RESULTS: Using microsatellite enrichm...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Botanical Society of America
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5001860/ https://www.ncbi.nlm.nih.gov/pubmed/27610278 http://dx.doi.org/10.3732/apps.1600034 |
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author | Martos, Florent Lebreton, Gérard Rivière, Eric Humeau, Laurence Chevallier, Marie-Hélène |
author_facet | Martos, Florent Lebreton, Gérard Rivière, Eric Humeau, Laurence Chevallier, Marie-Hélène |
author_sort | Martos, Florent |
collection | PubMed |
description | PREMISE OF THE STUDY: Polymorphic markers were required for a native tree of the Mascarene Islands, Foetidia mauritiana (Lecythidaceae), to investigate the effects of fragmentation of lowland tropical habitats on tree mating systems and on gene flow. METHODS AND RESULTS: Using microsatellite enrichment and next-generation sequencing, we identified 13 microsatellite loci (dinucleotide repeats). They were highly polymorphic in 121 trees sampled in the largest three populations on Réunion, revealing 2–17 different alleles per locus. Furthermore, they were found to be polymorphic in conspecific populations on Mauritius and in F. rodriguesiana from Rodrigues. CONCLUSIONS: These results indicate the utility of these markers to investigate genetic diversity, mating systems, and gene flow in a genus native to the biodiversity hotspot of Madagascar and the Indian Ocean islands. |
format | Online Article Text |
id | pubmed-5001860 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Botanical Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-50018602016-09-08 Microsatellites in the tree Foetidia mauritiana (Lecythidaceae) and utility in other Foetidia taxa from the Mascarene Islands(1) Martos, Florent Lebreton, Gérard Rivière, Eric Humeau, Laurence Chevallier, Marie-Hélène Appl Plant Sci Primer Note PREMISE OF THE STUDY: Polymorphic markers were required for a native tree of the Mascarene Islands, Foetidia mauritiana (Lecythidaceae), to investigate the effects of fragmentation of lowland tropical habitats on tree mating systems and on gene flow. METHODS AND RESULTS: Using microsatellite enrichment and next-generation sequencing, we identified 13 microsatellite loci (dinucleotide repeats). They were highly polymorphic in 121 trees sampled in the largest three populations on Réunion, revealing 2–17 different alleles per locus. Furthermore, they were found to be polymorphic in conspecific populations on Mauritius and in F. rodriguesiana from Rodrigues. CONCLUSIONS: These results indicate the utility of these markers to investigate genetic diversity, mating systems, and gene flow in a genus native to the biodiversity hotspot of Madagascar and the Indian Ocean islands. Botanical Society of America 2016-08-19 /pmc/articles/PMC5001860/ /pubmed/27610278 http://dx.doi.org/10.3732/apps.1600034 Text en © 2016 Martos et al. Published by the Botanical Society of America http://creativecommons.org/licenses/by-nc/4.0/ This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA). |
spellingShingle | Primer Note Martos, Florent Lebreton, Gérard Rivière, Eric Humeau, Laurence Chevallier, Marie-Hélène Microsatellites in the tree Foetidia mauritiana (Lecythidaceae) and utility in other Foetidia taxa from the Mascarene Islands(1) |
title | Microsatellites in the tree Foetidia mauritiana (Lecythidaceae) and utility in other Foetidia taxa from the Mascarene Islands(1) |
title_full | Microsatellites in the tree Foetidia mauritiana (Lecythidaceae) and utility in other Foetidia taxa from the Mascarene Islands(1) |
title_fullStr | Microsatellites in the tree Foetidia mauritiana (Lecythidaceae) and utility in other Foetidia taxa from the Mascarene Islands(1) |
title_full_unstemmed | Microsatellites in the tree Foetidia mauritiana (Lecythidaceae) and utility in other Foetidia taxa from the Mascarene Islands(1) |
title_short | Microsatellites in the tree Foetidia mauritiana (Lecythidaceae) and utility in other Foetidia taxa from the Mascarene Islands(1) |
title_sort | microsatellites in the tree foetidia mauritiana (lecythidaceae) and utility in other foetidia taxa from the mascarene islands(1) |
topic | Primer Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5001860/ https://www.ncbi.nlm.nih.gov/pubmed/27610278 http://dx.doi.org/10.3732/apps.1600034 |
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