Cargando…

Isolation and Metagenomic Identification of Avian Leukosis Virus Associated with Mortality in Broiler Chicken

Avian leukosis virus (ALV) belongs to the family Retroviridae and causes considerable economic losses to the poultry industry. Following an outbreak associated with high mortality in a broiler flock in northern part of Malaysia, kidney tissues from affected chickens were submitted for virus isolatio...

Descripción completa

Detalles Bibliográficos
Autores principales: Bande, Faruku, Arshad, Siti Suri, Omar, Abdul Rahman
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5002465/
https://www.ncbi.nlm.nih.gov/pubmed/27597865
http://dx.doi.org/10.1155/2016/9058403
_version_ 1782450572149915648
author Bande, Faruku
Arshad, Siti Suri
Omar, Abdul Rahman
author_facet Bande, Faruku
Arshad, Siti Suri
Omar, Abdul Rahman
author_sort Bande, Faruku
collection PubMed
description Avian leukosis virus (ALV) belongs to the family Retroviridae and causes considerable economic losses to the poultry industry. Following an outbreak associated with high mortality in a broiler flock in northern part of Malaysia, kidney tissues from affected chickens were submitted for virus isolation and identification in chicken embryonated egg and MDCK cells. Evidence of virus growth was indicated by haemorrhage and embryo mortality in egg culture. While viral growth in cell culture was evidenced by the development of cytopathic effects. The isolated virus was purified by sucrose gradient and identified using negative staining transmission electron microscopy. Further confirmation was achieved through next-generation sequencing and nucleotide sequence homology search. Analysis of the viral sequences using the NCBI BLAST tool revealed 99-100% sequence homology with exogenous ALV viral envelope protein. Phylogenetic analysis based on partial envelope sequences showed the Malaysian isolate clustered with Taiwanese and Japanese ALV strains, which were closer to ALV subgroup J, ALV subgroup E, and recombinant A/E isolates. Based on these findings, ALV was concluded to be associated with the present outbreak. It was recommended that further studies should be conducted on the molecular epidemiology and pathogenicity of the identified virus isolate.
format Online
Article
Text
id pubmed-5002465
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-50024652016-09-05 Isolation and Metagenomic Identification of Avian Leukosis Virus Associated with Mortality in Broiler Chicken Bande, Faruku Arshad, Siti Suri Omar, Abdul Rahman Adv Virol Research Article Avian leukosis virus (ALV) belongs to the family Retroviridae and causes considerable economic losses to the poultry industry. Following an outbreak associated with high mortality in a broiler flock in northern part of Malaysia, kidney tissues from affected chickens were submitted for virus isolation and identification in chicken embryonated egg and MDCK cells. Evidence of virus growth was indicated by haemorrhage and embryo mortality in egg culture. While viral growth in cell culture was evidenced by the development of cytopathic effects. The isolated virus was purified by sucrose gradient and identified using negative staining transmission electron microscopy. Further confirmation was achieved through next-generation sequencing and nucleotide sequence homology search. Analysis of the viral sequences using the NCBI BLAST tool revealed 99-100% sequence homology with exogenous ALV viral envelope protein. Phylogenetic analysis based on partial envelope sequences showed the Malaysian isolate clustered with Taiwanese and Japanese ALV strains, which were closer to ALV subgroup J, ALV subgroup E, and recombinant A/E isolates. Based on these findings, ALV was concluded to be associated with the present outbreak. It was recommended that further studies should be conducted on the molecular epidemiology and pathogenicity of the identified virus isolate. Hindawi Publishing Corporation 2016 2016-08-15 /pmc/articles/PMC5002465/ /pubmed/27597865 http://dx.doi.org/10.1155/2016/9058403 Text en Copyright © 2016 Faruku Bande et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Bande, Faruku
Arshad, Siti Suri
Omar, Abdul Rahman
Isolation and Metagenomic Identification of Avian Leukosis Virus Associated with Mortality in Broiler Chicken
title Isolation and Metagenomic Identification of Avian Leukosis Virus Associated with Mortality in Broiler Chicken
title_full Isolation and Metagenomic Identification of Avian Leukosis Virus Associated with Mortality in Broiler Chicken
title_fullStr Isolation and Metagenomic Identification of Avian Leukosis Virus Associated with Mortality in Broiler Chicken
title_full_unstemmed Isolation and Metagenomic Identification of Avian Leukosis Virus Associated with Mortality in Broiler Chicken
title_short Isolation and Metagenomic Identification of Avian Leukosis Virus Associated with Mortality in Broiler Chicken
title_sort isolation and metagenomic identification of avian leukosis virus associated with mortality in broiler chicken
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5002465/
https://www.ncbi.nlm.nih.gov/pubmed/27597865
http://dx.doi.org/10.1155/2016/9058403
work_keys_str_mv AT bandefaruku isolationandmetagenomicidentificationofavianleukosisvirusassociatedwithmortalityinbroilerchicken
AT arshadsitisuri isolationandmetagenomicidentificationofavianleukosisvirusassociatedwithmortalityinbroilerchicken
AT omarabdulrahman isolationandmetagenomicidentificationofavianleukosisvirusassociatedwithmortalityinbroilerchicken