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DNA replication origins—where do we begin?

For more than three decades, investigators have sought to identify the precise locations where DNA replication initiates in mammalian genomes. The development of molecular and biochemical approaches to identify start sites of DNA replication (origins) based on the presence of defining and characteri...

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Detalles Bibliográficos
Autores principales: Prioleau, Marie-Noëlle, MacAlpine, David M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5002974/
https://www.ncbi.nlm.nih.gov/pubmed/27542827
http://dx.doi.org/10.1101/gad.285114.116
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author Prioleau, Marie-Noëlle
MacAlpine, David M.
author_facet Prioleau, Marie-Noëlle
MacAlpine, David M.
author_sort Prioleau, Marie-Noëlle
collection PubMed
description For more than three decades, investigators have sought to identify the precise locations where DNA replication initiates in mammalian genomes. The development of molecular and biochemical approaches to identify start sites of DNA replication (origins) based on the presence of defining and characteristic replication intermediates at specific loci led to the identification of only a handful of mammalian replication origins. The limited number of identified origins prevented a comprehensive and exhaustive search for conserved genomic features that were capable of specifying origins of DNA replication. More recently, the adaptation of origin-mapping assays to genome-wide approaches has led to the identification of tens of thousands of replication origins throughout mammalian genomes, providing an unprecedented opportunity to identify both genetic and epigenetic features that define and regulate their distribution and utilization. Here we summarize recent advances in our understanding of how primary sequence, chromatin environment, and nuclear architecture contribute to the dynamic selection and activation of replication origins across diverse cell types and developmental stages.
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spelling pubmed-50029742017-02-01 DNA replication origins—where do we begin? Prioleau, Marie-Noëlle MacAlpine, David M. Genes Dev Review For more than three decades, investigators have sought to identify the precise locations where DNA replication initiates in mammalian genomes. The development of molecular and biochemical approaches to identify start sites of DNA replication (origins) based on the presence of defining and characteristic replication intermediates at specific loci led to the identification of only a handful of mammalian replication origins. The limited number of identified origins prevented a comprehensive and exhaustive search for conserved genomic features that were capable of specifying origins of DNA replication. More recently, the adaptation of origin-mapping assays to genome-wide approaches has led to the identification of tens of thousands of replication origins throughout mammalian genomes, providing an unprecedented opportunity to identify both genetic and epigenetic features that define and regulate their distribution and utilization. Here we summarize recent advances in our understanding of how primary sequence, chromatin environment, and nuclear architecture contribute to the dynamic selection and activation of replication origins across diverse cell types and developmental stages. Cold Spring Harbor Laboratory Press 2016-08-01 /pmc/articles/PMC5002974/ /pubmed/27542827 http://dx.doi.org/10.1101/gad.285114.116 Text en © 2016 Prioleau and MacAlpine; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.
spellingShingle Review
Prioleau, Marie-Noëlle
MacAlpine, David M.
DNA replication origins—where do we begin?
title DNA replication origins—where do we begin?
title_full DNA replication origins—where do we begin?
title_fullStr DNA replication origins—where do we begin?
title_full_unstemmed DNA replication origins—where do we begin?
title_short DNA replication origins—where do we begin?
title_sort dna replication origins—where do we begin?
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5002974/
https://www.ncbi.nlm.nih.gov/pubmed/27542827
http://dx.doi.org/10.1101/gad.285114.116
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