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Spheroid Formation of Hepatocarcinoma Cells in Microwells: Experiments and Monte Carlo Simulations
The formation of spherical aggregates during the growth of cell population has long been observed under various conditions. We observed the formation of such aggregates during proliferation of Huh-7.5 cells, a human hepatocarcinoma cell line, in a microfabricated low-adhesion microwell system (Spher...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5003351/ https://www.ncbi.nlm.nih.gov/pubmed/27571565 http://dx.doi.org/10.1371/journal.pone.0161915 |
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author | Wang, Yan Kim, Myung Hee Tabaei, Seyed R. Park, Jae Hyeok Na, Kyuhwan Chung, Seok Zhdanov, Vladimir P. Cho, Nam-Joon |
author_facet | Wang, Yan Kim, Myung Hee Tabaei, Seyed R. Park, Jae Hyeok Na, Kyuhwan Chung, Seok Zhdanov, Vladimir P. Cho, Nam-Joon |
author_sort | Wang, Yan |
collection | PubMed |
description | The formation of spherical aggregates during the growth of cell population has long been observed under various conditions. We observed the formation of such aggregates during proliferation of Huh-7.5 cells, a human hepatocarcinoma cell line, in a microfabricated low-adhesion microwell system (SpheroFilm; formed of mass-producible silicone elastomer) on the length scales up to 500 μm. The cell proliferation was also tracked with immunofluorescence staining of F-actin and cell proliferation marker Ki-67. Meanwhile, our complementary 3D Monte Carlo simulations, taking cell diffusion and division, cell-cell and cell-scaffold adhesion, and gravity into account, illustrate the role of these factors in the formation of spheroids. Taken together, our experimental and simulation results provide an integrative view of the process of spheroid formation for Huh-7.5 cells. |
format | Online Article Text |
id | pubmed-5003351 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-50033512016-09-12 Spheroid Formation of Hepatocarcinoma Cells in Microwells: Experiments and Monte Carlo Simulations Wang, Yan Kim, Myung Hee Tabaei, Seyed R. Park, Jae Hyeok Na, Kyuhwan Chung, Seok Zhdanov, Vladimir P. Cho, Nam-Joon PLoS One Research Article The formation of spherical aggregates during the growth of cell population has long been observed under various conditions. We observed the formation of such aggregates during proliferation of Huh-7.5 cells, a human hepatocarcinoma cell line, in a microfabricated low-adhesion microwell system (SpheroFilm; formed of mass-producible silicone elastomer) on the length scales up to 500 μm. The cell proliferation was also tracked with immunofluorescence staining of F-actin and cell proliferation marker Ki-67. Meanwhile, our complementary 3D Monte Carlo simulations, taking cell diffusion and division, cell-cell and cell-scaffold adhesion, and gravity into account, illustrate the role of these factors in the formation of spheroids. Taken together, our experimental and simulation results provide an integrative view of the process of spheroid formation for Huh-7.5 cells. Public Library of Science 2016-08-29 /pmc/articles/PMC5003351/ /pubmed/27571565 http://dx.doi.org/10.1371/journal.pone.0161915 Text en © 2016 Wang et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Wang, Yan Kim, Myung Hee Tabaei, Seyed R. Park, Jae Hyeok Na, Kyuhwan Chung, Seok Zhdanov, Vladimir P. Cho, Nam-Joon Spheroid Formation of Hepatocarcinoma Cells in Microwells: Experiments and Monte Carlo Simulations |
title | Spheroid Formation of Hepatocarcinoma Cells in Microwells: Experiments and Monte Carlo Simulations |
title_full | Spheroid Formation of Hepatocarcinoma Cells in Microwells: Experiments and Monte Carlo Simulations |
title_fullStr | Spheroid Formation of Hepatocarcinoma Cells in Microwells: Experiments and Monte Carlo Simulations |
title_full_unstemmed | Spheroid Formation of Hepatocarcinoma Cells in Microwells: Experiments and Monte Carlo Simulations |
title_short | Spheroid Formation of Hepatocarcinoma Cells in Microwells: Experiments and Monte Carlo Simulations |
title_sort | spheroid formation of hepatocarcinoma cells in microwells: experiments and monte carlo simulations |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5003351/ https://www.ncbi.nlm.nih.gov/pubmed/27571565 http://dx.doi.org/10.1371/journal.pone.0161915 |
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