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Testing a Microarray to Detect and Monitor Toxic Microalgae in Arcachon Bay in France
Harmful algal blooms (HABs) occur worldwide, causing health problems and economic damages to fisheries and tourism. Monitoring agencies are therefore essential, yet monitoring is based only on time-consuming light microscopy, a level at which a correct identification can be limited by insufficient m...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5003436/ https://www.ncbi.nlm.nih.gov/pubmed/27605178 http://dx.doi.org/10.3390/microarrays2010001 |
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author | Kegel, Jessica U. Del Amo, Yolanda Costes, Laurence Medlin, Linda K. |
author_facet | Kegel, Jessica U. Del Amo, Yolanda Costes, Laurence Medlin, Linda K. |
author_sort | Kegel, Jessica U. |
collection | PubMed |
description | Harmful algal blooms (HABs) occur worldwide, causing health problems and economic damages to fisheries and tourism. Monitoring agencies are therefore essential, yet monitoring is based only on time-consuming light microscopy, a level at which a correct identification can be limited by insufficient morphological characters. The project MIDTAL (Microarray Detection of Toxic Algae)—an FP7-funded EU project—used rRNA genes (SSU and LSU) as a target on microarrays to identify toxic species. Furthermore, toxins were detected with a newly developed multiplex optical Surface Plasmon Resonance biosensor (Multi SPR) and compared with an enzyme-linked immunosorbent assay (ELISA). In this study, we demonstrate the latest generation of MIDTAL microarrays (version 3) and show the correlation between cell counts, detected toxin and microarray signals from field samples taken in Arcachon Bay in France in 2011. The MIDTAL microarray always detected more potentially toxic species than those detected by microscopic counts. The toxin detection was even more sensitive than both methods. Because of the universal nature of both toxin and species microarrays, they can be used to detect invasive species. Nevertheless, the MIDTAL microarray is not completely universal: first, because not all toxic species are on the chip, and second, because invasive species, such as Ostreopsis, already influence European coasts. |
format | Online Article Text |
id | pubmed-5003436 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-50034362016-09-06 Testing a Microarray to Detect and Monitor Toxic Microalgae in Arcachon Bay in France Kegel, Jessica U. Del Amo, Yolanda Costes, Laurence Medlin, Linda K. Microarrays (Basel) Article Harmful algal blooms (HABs) occur worldwide, causing health problems and economic damages to fisheries and tourism. Monitoring agencies are therefore essential, yet monitoring is based only on time-consuming light microscopy, a level at which a correct identification can be limited by insufficient morphological characters. The project MIDTAL (Microarray Detection of Toxic Algae)—an FP7-funded EU project—used rRNA genes (SSU and LSU) as a target on microarrays to identify toxic species. Furthermore, toxins were detected with a newly developed multiplex optical Surface Plasmon Resonance biosensor (Multi SPR) and compared with an enzyme-linked immunosorbent assay (ELISA). In this study, we demonstrate the latest generation of MIDTAL microarrays (version 3) and show the correlation between cell counts, detected toxin and microarray signals from field samples taken in Arcachon Bay in France in 2011. The MIDTAL microarray always detected more potentially toxic species than those detected by microscopic counts. The toxin detection was even more sensitive than both methods. Because of the universal nature of both toxin and species microarrays, they can be used to detect invasive species. Nevertheless, the MIDTAL microarray is not completely universal: first, because not all toxic species are on the chip, and second, because invasive species, such as Ostreopsis, already influence European coasts. MDPI 2013-03-05 /pmc/articles/PMC5003436/ /pubmed/27605178 http://dx.doi.org/10.3390/microarrays2010001 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Kegel, Jessica U. Del Amo, Yolanda Costes, Laurence Medlin, Linda K. Testing a Microarray to Detect and Monitor Toxic Microalgae in Arcachon Bay in France |
title | Testing a Microarray to Detect and Monitor Toxic Microalgae in Arcachon Bay in France |
title_full | Testing a Microarray to Detect and Monitor Toxic Microalgae in Arcachon Bay in France |
title_fullStr | Testing a Microarray to Detect and Monitor Toxic Microalgae in Arcachon Bay in France |
title_full_unstemmed | Testing a Microarray to Detect and Monitor Toxic Microalgae in Arcachon Bay in France |
title_short | Testing a Microarray to Detect and Monitor Toxic Microalgae in Arcachon Bay in France |
title_sort | testing a microarray to detect and monitor toxic microalgae in arcachon bay in france |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5003436/ https://www.ncbi.nlm.nih.gov/pubmed/27605178 http://dx.doi.org/10.3390/microarrays2010001 |
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