Cargando…

Overview on Techniques to Construct Tissue Arrays with Special Emphasis on Tissue Microarrays

With the advent of new histopathological staining techniques (histochemistry, immunohistochemistry, in situ hybridization) and the discovery of thousands of new genes, mRNA, and proteins by molecular biology, the need grew for a technique to compare many different cells or tissues on one slide in a...

Descripción completa

Detalles Bibliográficos
Autor principal: Vogel, Ulrich
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5003444/
https://www.ncbi.nlm.nih.gov/pubmed/27600339
http://dx.doi.org/10.3390/microarrays3020103
_version_ 1782450647875977216
author Vogel, Ulrich
author_facet Vogel, Ulrich
author_sort Vogel, Ulrich
collection PubMed
description With the advent of new histopathological staining techniques (histochemistry, immunohistochemistry, in situ hybridization) and the discovery of thousands of new genes, mRNA, and proteins by molecular biology, the need grew for a technique to compare many different cells or tissues on one slide in a cost effective manner and with the possibility to easily track the identity of each specimen: the tissue array (TA). Basically, a TA consists of at least two different specimens per slide. TAs differ in the kind of specimens, the number of specimens installed, the dimension of the specimens, the arrangement of the specimens, the embedding medium, the technique to prepare the specimens to be installed, and the technique to construct the TA itself. A TA can be constructed by arranging the tissue specimens in a mold and subsequently pouring the mold with the embedding medium of choice. In contrast, preformed so-called recipient blocks consisting of the embedding medium of choice have punched, drilled, or poured holes of different diameters and distances in which the cells or tissue biopsies will be deployed manually, semi-automatically, or automatically. The costs of constructing a TA differ from a few to thousands of Euros depending on the technique/equipment used. Remarkably high quality TAs can be also achieved by low cost techniques.
format Online
Article
Text
id pubmed-5003444
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-50034442016-09-06 Overview on Techniques to Construct Tissue Arrays with Special Emphasis on Tissue Microarrays Vogel, Ulrich Microarrays (Basel) Review With the advent of new histopathological staining techniques (histochemistry, immunohistochemistry, in situ hybridization) and the discovery of thousands of new genes, mRNA, and proteins by molecular biology, the need grew for a technique to compare many different cells or tissues on one slide in a cost effective manner and with the possibility to easily track the identity of each specimen: the tissue array (TA). Basically, a TA consists of at least two different specimens per slide. TAs differ in the kind of specimens, the number of specimens installed, the dimension of the specimens, the arrangement of the specimens, the embedding medium, the technique to prepare the specimens to be installed, and the technique to construct the TA itself. A TA can be constructed by arranging the tissue specimens in a mold and subsequently pouring the mold with the embedding medium of choice. In contrast, preformed so-called recipient blocks consisting of the embedding medium of choice have punched, drilled, or poured holes of different diameters and distances in which the cells or tissue biopsies will be deployed manually, semi-automatically, or automatically. The costs of constructing a TA differ from a few to thousands of Euros depending on the technique/equipment used. Remarkably high quality TAs can be also achieved by low cost techniques. MDPI 2014-04-17 /pmc/articles/PMC5003444/ /pubmed/27600339 http://dx.doi.org/10.3390/microarrays3020103 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Review
Vogel, Ulrich
Overview on Techniques to Construct Tissue Arrays with Special Emphasis on Tissue Microarrays
title Overview on Techniques to Construct Tissue Arrays with Special Emphasis on Tissue Microarrays
title_full Overview on Techniques to Construct Tissue Arrays with Special Emphasis on Tissue Microarrays
title_fullStr Overview on Techniques to Construct Tissue Arrays with Special Emphasis on Tissue Microarrays
title_full_unstemmed Overview on Techniques to Construct Tissue Arrays with Special Emphasis on Tissue Microarrays
title_short Overview on Techniques to Construct Tissue Arrays with Special Emphasis on Tissue Microarrays
title_sort overview on techniques to construct tissue arrays with special emphasis on tissue microarrays
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5003444/
https://www.ncbi.nlm.nih.gov/pubmed/27600339
http://dx.doi.org/10.3390/microarrays3020103
work_keys_str_mv AT vogelulrich overviewontechniquestoconstructtissuearrayswithspecialemphasisontissuemicroarrays