A Double-Hybridization Approach for the Transcription- and Amplification-Free Detection of Specific mRNA on a Microarray

A double-hybridization approach was developed for the enzyme-free detection of specific mRNA of a housekeeping gene. Targeted mRNA was immobilized by hybridization to complementary DNA capture probes spotted onto a microarray. A second hybridization step of Cy5-conjugated label DNA to another sectio...

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Detalles Bibliográficos
Autores principales: Haider, Michaela, Haselgrübler, Thomas, Sonnleitner, Alois, Aberger, Fritz, Hesse, Jan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5003450/
https://www.ncbi.nlm.nih.gov/pubmed/27600071
http://dx.doi.org/10.3390/microarrays5010005

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5003450/
https://www.ncbi.nlm.nih.gov/pubmed/27600071
http://dx.doi.org/10.3390/microarrays5010005

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