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Comparative Analyses of MicroRNA Microarrays during Cardiogenesis: Functional Perspectives

Cardiovascular development is a complex process in which several transcriptional pathways are operative, providing instructions to the developing cardiomyocytes, while coping with contraction and morphogenetic movements to shape the mature heart. The discovery of microRNAs has added a new layer of c...

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Detalles Bibliográficos
Autores principales: Bonet, Fernando, Hernandez-Torres, Francisco, Esteban, Franciso J., Aranega, Amelia, Franco, Diego
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5003481/
https://www.ncbi.nlm.nih.gov/pubmed/27605182
http://dx.doi.org/10.3390/microarrays2020081
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author Bonet, Fernando
Hernandez-Torres, Francisco
Esteban, Franciso J.
Aranega, Amelia
Franco, Diego
author_facet Bonet, Fernando
Hernandez-Torres, Francisco
Esteban, Franciso J.
Aranega, Amelia
Franco, Diego
author_sort Bonet, Fernando
collection PubMed
description Cardiovascular development is a complex process in which several transcriptional pathways are operative, providing instructions to the developing cardiomyocytes, while coping with contraction and morphogenetic movements to shape the mature heart. The discovery of microRNAs has added a new layer of complexity to the molecular mechanisms governing the formation of the heart. Discrete genetic ablation of the microRNAs processing enzymes, such as Dicer and Drosha, has highlighted the functional roles of microRNAs during heart development. Importantly, selective deletion of a single microRNA, miR-1-2, results in an embryonic lethal phenotype in which both morphogenetic, as well as impaired conduction, phenotypes can be observed. In an effort to grasp the variability of microRNA expression during cardiac morphogenesis, we recently reported the dynamic expression profile during ventricular development, highlighting the importance of miR-27 on the regulation of a key cardiac transcription factor, Mef2c. In this review, we compare the microRNA expression profile in distinct models of cardiogenesis, such as ventricular chamber development, induced pluripotent stem cell (iPS)-derived cardiomyocytes and the aging heart. Importantly, out of 486 microRNAs assessed in the developing heart, 11% (55) displayed increased expression, many of which are also differentially expressed in distinct cardiogenetic experimental models, including iPS-derived cardiomyocytes. A review on the functional analyses of these differentially expressed microRNAs will be provided in the context of cardiac development, highlighting the resolution and power of microarrays analyses on the quest to decipher the most relevant microRNAs in the developing, aging and diseased heart.
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spelling pubmed-50034812016-09-06 Comparative Analyses of MicroRNA Microarrays during Cardiogenesis: Functional Perspectives Bonet, Fernando Hernandez-Torres, Francisco Esteban, Franciso J. Aranega, Amelia Franco, Diego Microarrays (Basel) Review Cardiovascular development is a complex process in which several transcriptional pathways are operative, providing instructions to the developing cardiomyocytes, while coping with contraction and morphogenetic movements to shape the mature heart. The discovery of microRNAs has added a new layer of complexity to the molecular mechanisms governing the formation of the heart. Discrete genetic ablation of the microRNAs processing enzymes, such as Dicer and Drosha, has highlighted the functional roles of microRNAs during heart development. Importantly, selective deletion of a single microRNA, miR-1-2, results in an embryonic lethal phenotype in which both morphogenetic, as well as impaired conduction, phenotypes can be observed. In an effort to grasp the variability of microRNA expression during cardiac morphogenesis, we recently reported the dynamic expression profile during ventricular development, highlighting the importance of miR-27 on the regulation of a key cardiac transcription factor, Mef2c. In this review, we compare the microRNA expression profile in distinct models of cardiogenesis, such as ventricular chamber development, induced pluripotent stem cell (iPS)-derived cardiomyocytes and the aging heart. Importantly, out of 486 microRNAs assessed in the developing heart, 11% (55) displayed increased expression, many of which are also differentially expressed in distinct cardiogenetic experimental models, including iPS-derived cardiomyocytes. A review on the functional analyses of these differentially expressed microRNAs will be provided in the context of cardiac development, highlighting the resolution and power of microarrays analyses on the quest to decipher the most relevant microRNAs in the developing, aging and diseased heart. MDPI 2013-04-03 /pmc/articles/PMC5003481/ /pubmed/27605182 http://dx.doi.org/10.3390/microarrays2020081 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Review
Bonet, Fernando
Hernandez-Torres, Francisco
Esteban, Franciso J.
Aranega, Amelia
Franco, Diego
Comparative Analyses of MicroRNA Microarrays during Cardiogenesis: Functional Perspectives
title Comparative Analyses of MicroRNA Microarrays during Cardiogenesis: Functional Perspectives
title_full Comparative Analyses of MicroRNA Microarrays during Cardiogenesis: Functional Perspectives
title_fullStr Comparative Analyses of MicroRNA Microarrays during Cardiogenesis: Functional Perspectives
title_full_unstemmed Comparative Analyses of MicroRNA Microarrays during Cardiogenesis: Functional Perspectives
title_short Comparative Analyses of MicroRNA Microarrays during Cardiogenesis: Functional Perspectives
title_sort comparative analyses of microrna microarrays during cardiogenesis: functional perspectives
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5003481/
https://www.ncbi.nlm.nih.gov/pubmed/27605182
http://dx.doi.org/10.3390/microarrays2020081
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