Immobilized lipase catalyzing glucose stearate synthesis and their surfactant properties analysis

Sugar fatty acid esters are practical importance and have a variety of applications that include surfactants and as an emulsifying agent. In this study, we report glucose stearate synthesis using lipase-Fe(3)O(4) nanoparticles catalyst. The influence of various reaction factors, such as silica gel c...

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Autores principales: Sebatini, A. Maria, Jain, Manisha, Radha, P., Kiruthika, S., Tamilarasan, Krishnamurthi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2016
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5003783/
https://www.ncbi.nlm.nih.gov/pubmed/28330256
http://dx.doi.org/10.1007/s13205-016-0501-z
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author Sebatini, A. Maria
Jain, Manisha
Radha, P.
Kiruthika, S.
Tamilarasan, Krishnamurthi
author_facet Sebatini, A. Maria
Jain, Manisha
Radha, P.
Kiruthika, S.
Tamilarasan, Krishnamurthi
author_sort Sebatini, A. Maria
collection PubMed
description Sugar fatty acid esters are practical importance and have a variety of applications that include surfactants and as an emulsifying agent. In this study, we report glucose stearate synthesis using lipase-Fe(3)O(4) nanoparticles catalyst. The influence of various reaction factors, such as silica gel concentration, molar ratio of sugar/acid, reaction temperature and speed of agitation on esterification by immobilized enzyme was analyzed. The glucose stearate esterification degree of 87.2 % was obtained under the optimized condition: 1:2 molar ratio of glucose/stearic acid, 2 % (w/v) of silica gel at 120 rpm and 40 °C. Glucose esters were characterized according to their surfactant activity like emulsification index, oil displacement activity and antimicrobial activity. The results indicated glucose stearate acts as biosurfactant, with emulsification index of 66 % in mustard oil and oil displacement activity of 19.64 cm(2).
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spelling pubmed-50037832016-09-02 Immobilized lipase catalyzing glucose stearate synthesis and their surfactant properties analysis Sebatini, A. Maria Jain, Manisha Radha, P. Kiruthika, S. Tamilarasan, Krishnamurthi 3 Biotech Original Article Sugar fatty acid esters are practical importance and have a variety of applications that include surfactants and as an emulsifying agent. In this study, we report glucose stearate synthesis using lipase-Fe(3)O(4) nanoparticles catalyst. The influence of various reaction factors, such as silica gel concentration, molar ratio of sugar/acid, reaction temperature and speed of agitation on esterification by immobilized enzyme was analyzed. The glucose stearate esterification degree of 87.2 % was obtained under the optimized condition: 1:2 molar ratio of glucose/stearic acid, 2 % (w/v) of silica gel at 120 rpm and 40 °C. Glucose esters were characterized according to their surfactant activity like emulsification index, oil displacement activity and antimicrobial activity. The results indicated glucose stearate acts as biosurfactant, with emulsification index of 66 % in mustard oil and oil displacement activity of 19.64 cm(2). Springer Berlin Heidelberg 2016-08-29 2016-12 /pmc/articles/PMC5003783/ /pubmed/28330256 http://dx.doi.org/10.1007/s13205-016-0501-z Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Sebatini, A. Maria
Jain, Manisha
Radha, P.
Kiruthika, S.
Tamilarasan, Krishnamurthi
Immobilized lipase catalyzing glucose stearate synthesis and their surfactant properties analysis
title Immobilized lipase catalyzing glucose stearate synthesis and their surfactant properties analysis
title_full Immobilized lipase catalyzing glucose stearate synthesis and their surfactant properties analysis
title_fullStr Immobilized lipase catalyzing glucose stearate synthesis and their surfactant properties analysis
title_full_unstemmed Immobilized lipase catalyzing glucose stearate synthesis and their surfactant properties analysis
title_short Immobilized lipase catalyzing glucose stearate synthesis and their surfactant properties analysis
title_sort immobilized lipase catalyzing glucose stearate synthesis and their surfactant properties analysis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5003783/
https://www.ncbi.nlm.nih.gov/pubmed/28330256
http://dx.doi.org/10.1007/s13205-016-0501-z
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