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The SOD Gene Family in Tomato: Identification, Phylogenetic Relationships, and Expression Patterns

Superoxide dismutases (SODs) are critical antioxidant enzymes that protect organisms from reactive oxygen species (ROS) caused by adverse conditions, and have been widely found in the cytoplasm, chloroplasts, and mitochondria of eukaryotic and prokaryotic cells. Tomato (Solanum lycopersicum L.) is a...

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Autores principales: Feng, Kun, Yu, Jiahong, Cheng, Yuan, Ruan, Meiying, Wang, Rongqing, Ye, Qingjing, Zhou, Guozhi, Li, Zhimiao, Yao, Zhuping, Yang, Yuejian, Zheng, Qingsong, Wan, Hongjian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5003820/
https://www.ncbi.nlm.nih.gov/pubmed/27625661
http://dx.doi.org/10.3389/fpls.2016.01279
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author Feng, Kun
Yu, Jiahong
Cheng, Yuan
Ruan, Meiying
Wang, Rongqing
Ye, Qingjing
Zhou, Guozhi
Li, Zhimiao
Yao, Zhuping
Yang, Yuejian
Zheng, Qingsong
Wan, Hongjian
author_facet Feng, Kun
Yu, Jiahong
Cheng, Yuan
Ruan, Meiying
Wang, Rongqing
Ye, Qingjing
Zhou, Guozhi
Li, Zhimiao
Yao, Zhuping
Yang, Yuejian
Zheng, Qingsong
Wan, Hongjian
author_sort Feng, Kun
collection PubMed
description Superoxide dismutases (SODs) are critical antioxidant enzymes that protect organisms from reactive oxygen species (ROS) caused by adverse conditions, and have been widely found in the cytoplasm, chloroplasts, and mitochondria of eukaryotic and prokaryotic cells. Tomato (Solanum lycopersicum L.) is an important economic crop and is cultivated worldwide. However, abiotic and biotic stresses severely hinder growth and development of the plant, which affects the production and quality of the crop. To reveal the potential roles of SOD genes under various stresses, we performed a systematic analysis of the tomato SOD gene family and analyzed the expression patterns of SlSOD genes in response to abiotic stresses at the whole-genome level. The characteristics of the SlSOD gene family were determined by analyzing gene structure, conserved motifs, chromosomal distribution, phylogenetic relationships, and expression patterns. We determined that there are at least nine SOD genes in tomato, including four Cu/ZnSODs, three FeSODs, and one MnSOD, and they are unevenly distributed on 12 chromosomes. Phylogenetic analyses of SOD genes from tomato and other plant species were separated into two groups with a high bootstrap value, indicating that these SOD genes were present before the monocot-dicot split. Additionally, many cis-elements that respond to different stresses were found in the promoters of nine SlSOD genes. Gene expression analysis based on RNA-seq data showed that most genes were expressed in all tested tissues, with the exception of SlSOD6 and SlSOD8, which were only expressed in young fruits. Microarray data analysis showed that most members of the SlSOD gene family were altered under salt- and drought-stress conditions. This genome-wide analysis of SlSOD genes helps to clarify the function of SlSOD genes under different stress conditions and provides information to aid in further understanding the evolutionary relationships of SOD genes in plants.
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spelling pubmed-50038202016-09-13 The SOD Gene Family in Tomato: Identification, Phylogenetic Relationships, and Expression Patterns Feng, Kun Yu, Jiahong Cheng, Yuan Ruan, Meiying Wang, Rongqing Ye, Qingjing Zhou, Guozhi Li, Zhimiao Yao, Zhuping Yang, Yuejian Zheng, Qingsong Wan, Hongjian Front Plant Sci Plant Science Superoxide dismutases (SODs) are critical antioxidant enzymes that protect organisms from reactive oxygen species (ROS) caused by adverse conditions, and have been widely found in the cytoplasm, chloroplasts, and mitochondria of eukaryotic and prokaryotic cells. Tomato (Solanum lycopersicum L.) is an important economic crop and is cultivated worldwide. However, abiotic and biotic stresses severely hinder growth and development of the plant, which affects the production and quality of the crop. To reveal the potential roles of SOD genes under various stresses, we performed a systematic analysis of the tomato SOD gene family and analyzed the expression patterns of SlSOD genes in response to abiotic stresses at the whole-genome level. The characteristics of the SlSOD gene family were determined by analyzing gene structure, conserved motifs, chromosomal distribution, phylogenetic relationships, and expression patterns. We determined that there are at least nine SOD genes in tomato, including four Cu/ZnSODs, three FeSODs, and one MnSOD, and they are unevenly distributed on 12 chromosomes. Phylogenetic analyses of SOD genes from tomato and other plant species were separated into two groups with a high bootstrap value, indicating that these SOD genes were present before the monocot-dicot split. Additionally, many cis-elements that respond to different stresses were found in the promoters of nine SlSOD genes. Gene expression analysis based on RNA-seq data showed that most genes were expressed in all tested tissues, with the exception of SlSOD6 and SlSOD8, which were only expressed in young fruits. Microarray data analysis showed that most members of the SlSOD gene family were altered under salt- and drought-stress conditions. This genome-wide analysis of SlSOD genes helps to clarify the function of SlSOD genes under different stress conditions and provides information to aid in further understanding the evolutionary relationships of SOD genes in plants. Frontiers Media S.A. 2016-08-30 /pmc/articles/PMC5003820/ /pubmed/27625661 http://dx.doi.org/10.3389/fpls.2016.01279 Text en Copyright © 2016 Feng, Yu, Cheng, Ruan, Wang, Ye, Zhou, Li, Yao, Yang, Zheng and Wan. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Feng, Kun
Yu, Jiahong
Cheng, Yuan
Ruan, Meiying
Wang, Rongqing
Ye, Qingjing
Zhou, Guozhi
Li, Zhimiao
Yao, Zhuping
Yang, Yuejian
Zheng, Qingsong
Wan, Hongjian
The SOD Gene Family in Tomato: Identification, Phylogenetic Relationships, and Expression Patterns
title The SOD Gene Family in Tomato: Identification, Phylogenetic Relationships, and Expression Patterns
title_full The SOD Gene Family in Tomato: Identification, Phylogenetic Relationships, and Expression Patterns
title_fullStr The SOD Gene Family in Tomato: Identification, Phylogenetic Relationships, and Expression Patterns
title_full_unstemmed The SOD Gene Family in Tomato: Identification, Phylogenetic Relationships, and Expression Patterns
title_short The SOD Gene Family in Tomato: Identification, Phylogenetic Relationships, and Expression Patterns
title_sort sod gene family in tomato: identification, phylogenetic relationships, and expression patterns
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5003820/
https://www.ncbi.nlm.nih.gov/pubmed/27625661
http://dx.doi.org/10.3389/fpls.2016.01279
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