Cargando…

Effects of Tocotrienols on Insulin Secretion-Associated Genes Expression of Rat Pancreatic Islets in a Dynamic Culture

Tocotrienols (T3) are well-known for their antioxidant properties besides showing therapeutic potential in clinical complications such as hyperlipidemia induced by diabetes. The aim of this study was to determine the effects of δ-T3, γ-T3, and α-T3 on insulin secretion-associated genes expression of...

Descripción completa

Detalles Bibliográficos
Autores principales: Chia, Ling L., Jantan, Ibrahim, Chua, Kien H., Lam, Kok W., Rullah, Kamal, Aluwi, Mohd F. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5003849/
https://www.ncbi.nlm.nih.gov/pubmed/27625609
http://dx.doi.org/10.3389/fphar.2016.00291
_version_ 1782450691312189440
author Chia, Ling L.
Jantan, Ibrahim
Chua, Kien H.
Lam, Kok W.
Rullah, Kamal
Aluwi, Mohd F. M.
author_facet Chia, Ling L.
Jantan, Ibrahim
Chua, Kien H.
Lam, Kok W.
Rullah, Kamal
Aluwi, Mohd F. M.
author_sort Chia, Ling L.
collection PubMed
description Tocotrienols (T3) are well-known for their antioxidant properties besides showing therapeutic potential in clinical complications such as hyperlipidemia induced by diabetes. The aim of this study was to determine the effects of δ-T3, γ-T3, and α-T3 on insulin secretion-associated genes expression of rat pancreatic islets in a dynamic culture. Pancreatic islets freshly isolated from male Wistar rats were treated with T3 for 1 h at 37°C in a microfluidic system with continuous operation. The cells were collected for total RNA extraction and reverse-transcribed, followed by measurement of insulin secretion-associated genes expression using quantitative real-time polymerase chain reaction. Molecular docking experiments were performed to gain insights on how the T3 bind to the receptors. Short-term exposure of δ- and γ-T3 to pancreatic β cells in a stimulant glucose condition (16.7 mM) significantly regulated preproinsulin mRNA levels and insulin gene transcription. In contrast, α-T3 possessed less ability in the activation of insulin synthesis level. Essentially, potassium chloride (KCl), a β cell membrane depolarising agent added into the treatment further enhanced the insulin production. δ- and γ-T3 revealed significantly higher quantitative expression in most of the insulin secretion-associated genes groups containing 16.7 mM glucose alone and 16.7 mM glucose with 30 mM KCl ranging from 600 to 1200 μM (p < 0.05). The findings suggest the potential of δ-T3 in regulating insulin synthesis and glucose-stimulated insulin secretion through triggering pathway especially in the presence of KCl.
format Online
Article
Text
id pubmed-5003849
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-50038492016-09-13 Effects of Tocotrienols on Insulin Secretion-Associated Genes Expression of Rat Pancreatic Islets in a Dynamic Culture Chia, Ling L. Jantan, Ibrahim Chua, Kien H. Lam, Kok W. Rullah, Kamal Aluwi, Mohd F. M. Front Pharmacol Pharmacology Tocotrienols (T3) are well-known for their antioxidant properties besides showing therapeutic potential in clinical complications such as hyperlipidemia induced by diabetes. The aim of this study was to determine the effects of δ-T3, γ-T3, and α-T3 on insulin secretion-associated genes expression of rat pancreatic islets in a dynamic culture. Pancreatic islets freshly isolated from male Wistar rats were treated with T3 for 1 h at 37°C in a microfluidic system with continuous operation. The cells were collected for total RNA extraction and reverse-transcribed, followed by measurement of insulin secretion-associated genes expression using quantitative real-time polymerase chain reaction. Molecular docking experiments were performed to gain insights on how the T3 bind to the receptors. Short-term exposure of δ- and γ-T3 to pancreatic β cells in a stimulant glucose condition (16.7 mM) significantly regulated preproinsulin mRNA levels and insulin gene transcription. In contrast, α-T3 possessed less ability in the activation of insulin synthesis level. Essentially, potassium chloride (KCl), a β cell membrane depolarising agent added into the treatment further enhanced the insulin production. δ- and γ-T3 revealed significantly higher quantitative expression in most of the insulin secretion-associated genes groups containing 16.7 mM glucose alone and 16.7 mM glucose with 30 mM KCl ranging from 600 to 1200 μM (p < 0.05). The findings suggest the potential of δ-T3 in regulating insulin synthesis and glucose-stimulated insulin secretion through triggering pathway especially in the presence of KCl. Frontiers Media S.A. 2016-08-30 /pmc/articles/PMC5003849/ /pubmed/27625609 http://dx.doi.org/10.3389/fphar.2016.00291 Text en Copyright © 2016 Chia, Jantan, Chua, Lam, Rullah and Aluwi. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Chia, Ling L.
Jantan, Ibrahim
Chua, Kien H.
Lam, Kok W.
Rullah, Kamal
Aluwi, Mohd F. M.
Effects of Tocotrienols on Insulin Secretion-Associated Genes Expression of Rat Pancreatic Islets in a Dynamic Culture
title Effects of Tocotrienols on Insulin Secretion-Associated Genes Expression of Rat Pancreatic Islets in a Dynamic Culture
title_full Effects of Tocotrienols on Insulin Secretion-Associated Genes Expression of Rat Pancreatic Islets in a Dynamic Culture
title_fullStr Effects of Tocotrienols on Insulin Secretion-Associated Genes Expression of Rat Pancreatic Islets in a Dynamic Culture
title_full_unstemmed Effects of Tocotrienols on Insulin Secretion-Associated Genes Expression of Rat Pancreatic Islets in a Dynamic Culture
title_short Effects of Tocotrienols on Insulin Secretion-Associated Genes Expression of Rat Pancreatic Islets in a Dynamic Culture
title_sort effects of tocotrienols on insulin secretion-associated genes expression of rat pancreatic islets in a dynamic culture
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5003849/
https://www.ncbi.nlm.nih.gov/pubmed/27625609
http://dx.doi.org/10.3389/fphar.2016.00291
work_keys_str_mv AT chialingl effectsoftocotrienolsoninsulinsecretionassociatedgenesexpressionofratpancreaticisletsinadynamicculture
AT jantanibrahim effectsoftocotrienolsoninsulinsecretionassociatedgenesexpressionofratpancreaticisletsinadynamicculture
AT chuakienh effectsoftocotrienolsoninsulinsecretionassociatedgenesexpressionofratpancreaticisletsinadynamicculture
AT lamkokw effectsoftocotrienolsoninsulinsecretionassociatedgenesexpressionofratpancreaticisletsinadynamicculture
AT rullahkamal effectsoftocotrienolsoninsulinsecretionassociatedgenesexpressionofratpancreaticisletsinadynamicculture
AT aluwimohdfm effectsoftocotrienolsoninsulinsecretionassociatedgenesexpressionofratpancreaticisletsinadynamicculture