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Causal Interrogation of Neuronal Networks and Behavior through Virally Transduced Ivermectin Receptors
The causal interrogation of neuronal networks involved in specific behaviors requires the spatially and temporally controlled modulation of neuronal activity. For long-term manipulation of neuronal activity, chemogenetic tools provide a reasonable alternative to short-term optogenetic approaches. He...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5004486/ https://www.ncbi.nlm.nih.gov/pubmed/27625595 http://dx.doi.org/10.3389/fnmol.2016.00075 |
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author | Obenhaus, Horst A. Rozov, Andrei Bertocchi, Ilaria Tang, Wannan Kirsch, Joachim Betz, Heinrich Sprengel, Rolf |
author_facet | Obenhaus, Horst A. Rozov, Andrei Bertocchi, Ilaria Tang, Wannan Kirsch, Joachim Betz, Heinrich Sprengel, Rolf |
author_sort | Obenhaus, Horst A. |
collection | PubMed |
description | The causal interrogation of neuronal networks involved in specific behaviors requires the spatially and temporally controlled modulation of neuronal activity. For long-term manipulation of neuronal activity, chemogenetic tools provide a reasonable alternative to short-term optogenetic approaches. Here we show that virus mediated gene transfer of the ivermectin (IVM) activated glycine receptor mutant GlyRα(1)(AG) can be used for the selective and reversible silencing of specific neuronal networks in mice. In the striatum, dorsal hippocampus, and olfactory bulb, GlyRα(1)(AG) promoted IVM dependent effects in representative behavioral assays. Moreover, GlyRα(1)(AG) mediated silencing had a strong and reversible impact on neuronal ensemble activity and c-Fos activation in the olfactory bulb. Together our results demonstrate that long-term, reversible and re-inducible neuronal silencing via GlyRα(1)(AG) is a promising tool for the interrogation of network mechanisms underlying the control of behavior and memory formation. |
format | Online Article Text |
id | pubmed-5004486 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-50044862016-09-13 Causal Interrogation of Neuronal Networks and Behavior through Virally Transduced Ivermectin Receptors Obenhaus, Horst A. Rozov, Andrei Bertocchi, Ilaria Tang, Wannan Kirsch, Joachim Betz, Heinrich Sprengel, Rolf Front Mol Neurosci Neuroscience The causal interrogation of neuronal networks involved in specific behaviors requires the spatially and temporally controlled modulation of neuronal activity. For long-term manipulation of neuronal activity, chemogenetic tools provide a reasonable alternative to short-term optogenetic approaches. Here we show that virus mediated gene transfer of the ivermectin (IVM) activated glycine receptor mutant GlyRα(1)(AG) can be used for the selective and reversible silencing of specific neuronal networks in mice. In the striatum, dorsal hippocampus, and olfactory bulb, GlyRα(1)(AG) promoted IVM dependent effects in representative behavioral assays. Moreover, GlyRα(1)(AG) mediated silencing had a strong and reversible impact on neuronal ensemble activity and c-Fos activation in the olfactory bulb. Together our results demonstrate that long-term, reversible and re-inducible neuronal silencing via GlyRα(1)(AG) is a promising tool for the interrogation of network mechanisms underlying the control of behavior and memory formation. Frontiers Media S.A. 2016-08-30 /pmc/articles/PMC5004486/ /pubmed/27625595 http://dx.doi.org/10.3389/fnmol.2016.00075 Text en Copyright © 2016 Obenhaus, Rozov, Bertocchi, Tang, Kirsch, Betz and Sprengel. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Neuroscience Obenhaus, Horst A. Rozov, Andrei Bertocchi, Ilaria Tang, Wannan Kirsch, Joachim Betz, Heinrich Sprengel, Rolf Causal Interrogation of Neuronal Networks and Behavior through Virally Transduced Ivermectin Receptors |
title | Causal Interrogation of Neuronal Networks and Behavior through Virally Transduced Ivermectin Receptors |
title_full | Causal Interrogation of Neuronal Networks and Behavior through Virally Transduced Ivermectin Receptors |
title_fullStr | Causal Interrogation of Neuronal Networks and Behavior through Virally Transduced Ivermectin Receptors |
title_full_unstemmed | Causal Interrogation of Neuronal Networks and Behavior through Virally Transduced Ivermectin Receptors |
title_short | Causal Interrogation of Neuronal Networks and Behavior through Virally Transduced Ivermectin Receptors |
title_sort | causal interrogation of neuronal networks and behavior through virally transduced ivermectin receptors |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5004486/ https://www.ncbi.nlm.nih.gov/pubmed/27625595 http://dx.doi.org/10.3389/fnmol.2016.00075 |
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