The Immunogenicity of Peptoid-Protein Conjugates

We demonstrate that a peptoid composed of five monomers and attached via a maleimide linker to a carrier protein elicits anti-peptoid, anti-linker and anti-carrier antibodies in rabbits. Specific anti-peptoid antibodies were affinity purified and used to reproducibly retrieve three specific peptoid-...

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Detalles Bibliográficos
Autores principales: Case, Allison, Desmond, Angela, Lopes, Daniel, Dye, Kelly, Mapes, Kelly, Ruback, Stephen, Pop, Iliodora, Kim, Jiyeun Kate, Chakravarty, Pavitra, Smallshaw, Joan E., Pop, Laurentiu M., Vitetta, Ellen S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5004597/
https://www.ncbi.nlm.nih.gov/pubmed/27595050
http://dx.doi.org/10.4172/2157-7560.1000329
Descripción
Sumario:We demonstrate that a peptoid composed of five monomers and attached via a maleimide linker to a carrier protein elicits anti-peptoid, anti-linker and anti-carrier antibodies in rabbits. Specific anti-peptoid antibodies were affinity purified and used to reproducibly retrieve three specific peptoid-coupled beads from 20,000 irrelevant peptoid-beads using magnetic screening.

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