The Immunogenicity of Peptoid-Protein Conjugates

We demonstrate that a peptoid composed of five monomers and attached via a maleimide linker to a carrier protein elicits anti-peptoid, anti-linker and anti-carrier antibodies in rabbits. Specific anti-peptoid antibodies were affinity purified and used to reproducibly retrieve three specific peptoid-...

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Autores principales: Case, Allison, Desmond, Angela, Lopes, Daniel, Dye, Kelly, Mapes, Kelly, Ruback, Stephen, Pop, Iliodora, Kim, Jiyeun Kate, Chakravarty, Pavitra, Smallshaw, Joan E., Pop, Laurentiu M., Vitetta, Ellen S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5004597/
https://www.ncbi.nlm.nih.gov/pubmed/27595050
http://dx.doi.org/10.4172/2157-7560.1000329
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author Case, Allison
Desmond, Angela
Lopes, Daniel
Dye, Kelly
Mapes, Kelly
Ruback, Stephen
Pop, Iliodora
Kim, Jiyeun Kate
Chakravarty, Pavitra
Smallshaw, Joan E.
Pop, Laurentiu M.
Vitetta, Ellen S.
author_facet Case, Allison
Desmond, Angela
Lopes, Daniel
Dye, Kelly
Mapes, Kelly
Ruback, Stephen
Pop, Iliodora
Kim, Jiyeun Kate
Chakravarty, Pavitra
Smallshaw, Joan E.
Pop, Laurentiu M.
Vitetta, Ellen S.
author_sort Case, Allison
collection PubMed
description We demonstrate that a peptoid composed of five monomers and attached via a maleimide linker to a carrier protein elicits anti-peptoid, anti-linker and anti-carrier antibodies in rabbits. Specific anti-peptoid antibodies were affinity purified and used to reproducibly retrieve three specific peptoid-coupled beads from 20,000 irrelevant peptoid-beads using magnetic screening.
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spelling pubmed-50045972016-09-01 The Immunogenicity of Peptoid-Protein Conjugates Case, Allison Desmond, Angela Lopes, Daniel Dye, Kelly Mapes, Kelly Ruback, Stephen Pop, Iliodora Kim, Jiyeun Kate Chakravarty, Pavitra Smallshaw, Joan E. Pop, Laurentiu M. Vitetta, Ellen S. J Vaccines Vaccin Article We demonstrate that a peptoid composed of five monomers and attached via a maleimide linker to a carrier protein elicits anti-peptoid, anti-linker and anti-carrier antibodies in rabbits. Specific anti-peptoid antibodies were affinity purified and used to reproducibly retrieve three specific peptoid-coupled beads from 20,000 irrelevant peptoid-beads using magnetic screening. 2016-07-11 2016-08 /pmc/articles/PMC5004597/ /pubmed/27595050 http://dx.doi.org/10.4172/2157-7560.1000329 Text en http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Article
Case, Allison
Desmond, Angela
Lopes, Daniel
Dye, Kelly
Mapes, Kelly
Ruback, Stephen
Pop, Iliodora
Kim, Jiyeun Kate
Chakravarty, Pavitra
Smallshaw, Joan E.
Pop, Laurentiu M.
Vitetta, Ellen S.
The Immunogenicity of Peptoid-Protein Conjugates
title The Immunogenicity of Peptoid-Protein Conjugates
title_full The Immunogenicity of Peptoid-Protein Conjugates
title_fullStr The Immunogenicity of Peptoid-Protein Conjugates
title_full_unstemmed The Immunogenicity of Peptoid-Protein Conjugates
title_short The Immunogenicity of Peptoid-Protein Conjugates
title_sort immunogenicity of peptoid-protein conjugates
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5004597/
https://www.ncbi.nlm.nih.gov/pubmed/27595050
http://dx.doi.org/10.4172/2157-7560.1000329
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