Ultra-superovulation for the CRISPR-Cas9-mediated production of gene-knockout, single-amino-acid-substituted, and floxed mice

Current advances in producing genetically modified mice using genome-editing technologies have indicated the need for improvement of limiting factors including zygote collection for microinjection and their cryopreservation. Recently, we developed a novel superovulation technique using inhibin antis...

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Detalles Bibliográficos
Autores principales: Nakagawa, Yoshiko, Sakuma, Tetsushi, Nishimichi, Norihisa, Yokosaki, Yasuyuki, Yanaka, Noriyuki, Takeo, Toru, Nakagata, Naomi, Yamamoto, Takashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2016
Materias:
Methods & Techniques
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5004614/
https://www.ncbi.nlm.nih.gov/pubmed/27387532
http://dx.doi.org/10.1242/bio.019349

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