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DNA methylation-mediated transcription factors regulate Piwil1 expression during chicken spermatogenesis
The P-element induced wimpy testis (Piwi) protein family is responsible for initiating spermatogenesis and maintaining the integrity of germ cells and stem cells, but little is known regarding its transcriptional regulation in poultry. Here, we characterized the methylation status of the Piwil1 prom...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Society for Reproduction and Development
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5004792/ https://www.ncbi.nlm.nih.gov/pubmed/27108736 http://dx.doi.org/10.1262/jrd.2016-003 |
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author | QIU, Lingling XU, Lu CHANG, Guobin GUO, Qixin LIU, Xiangping BI, Yulin ZHANG, Yu WANG, Hongzhi WANG, Kehua LU, Wei REN, Lichen ZHU, Pengfei WU, Yun ZHANG, Yang XU, Qi CHEN, Guohong |
author_facet | QIU, Lingling XU, Lu CHANG, Guobin GUO, Qixin LIU, Xiangping BI, Yulin ZHANG, Yu WANG, Hongzhi WANG, Kehua LU, Wei REN, Lichen ZHU, Pengfei WU, Yun ZHANG, Yang XU, Qi CHEN, Guohong |
author_sort | QIU, Lingling |
collection | PubMed |
description | The P-element induced wimpy testis (Piwi) protein family is responsible for initiating spermatogenesis and maintaining the integrity of germ cells and stem cells, but little is known regarding its transcriptional regulation in poultry. Here, we characterized the methylation status of the Piwil1 promoter in five different spermatogenic cell lines using direct bisulfite pyrosequencing and determined that methylation correlates negatively with germ cell type-specific expression patterns of piwil1. We demonstrated that methylation of the −148 CpG site, which is the predicted binding site for the transcription factors TCF3 and NRF1, was differentially methylated in different spermatogenic cells. This site was completely methylated in PGCs (primordial germ cells), but was unmethylated in round spermatids. A similar result was obtained in the region from +121 to +139 CpG sites of the Piwil1 promoter CpG island, which was predicted to contain SOX2 binding sites. In addition, demethylation assays further demonstrated that DNA methylation indeed regulates Piwil1 expression during chicken spermatogenesis. Combined with transcription factor binding site prediction, we speculate that methylation influences the recruitment of corresponding transcription factors. Collectively, we show the negative correlation between promoter methylation and piwil1 expression and that the spatiotemporal expression of chicken Piwil1 from the PGC stage to the round spermatid stage is influenced by methylation-mediated transcription factor regulation. |
format | Online Article Text |
id | pubmed-5004792 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | The Society for Reproduction and Development |
record_format | MEDLINE/PubMed |
spelling | pubmed-50047922016-08-31 DNA methylation-mediated transcription factors regulate Piwil1 expression during chicken spermatogenesis QIU, Lingling XU, Lu CHANG, Guobin GUO, Qixin LIU, Xiangping BI, Yulin ZHANG, Yu WANG, Hongzhi WANG, Kehua LU, Wei REN, Lichen ZHU, Pengfei WU, Yun ZHANG, Yang XU, Qi CHEN, Guohong J Reprod Dev Original Article The P-element induced wimpy testis (Piwi) protein family is responsible for initiating spermatogenesis and maintaining the integrity of germ cells and stem cells, but little is known regarding its transcriptional regulation in poultry. Here, we characterized the methylation status of the Piwil1 promoter in five different spermatogenic cell lines using direct bisulfite pyrosequencing and determined that methylation correlates negatively with germ cell type-specific expression patterns of piwil1. We demonstrated that methylation of the −148 CpG site, which is the predicted binding site for the transcription factors TCF3 and NRF1, was differentially methylated in different spermatogenic cells. This site was completely methylated in PGCs (primordial germ cells), but was unmethylated in round spermatids. A similar result was obtained in the region from +121 to +139 CpG sites of the Piwil1 promoter CpG island, which was predicted to contain SOX2 binding sites. In addition, demethylation assays further demonstrated that DNA methylation indeed regulates Piwil1 expression during chicken spermatogenesis. Combined with transcription factor binding site prediction, we speculate that methylation influences the recruitment of corresponding transcription factors. Collectively, we show the negative correlation between promoter methylation and piwil1 expression and that the spatiotemporal expression of chicken Piwil1 from the PGC stage to the round spermatid stage is influenced by methylation-mediated transcription factor regulation. The Society for Reproduction and Development 2016-04-25 2016-08 /pmc/articles/PMC5004792/ /pubmed/27108736 http://dx.doi.org/10.1262/jrd.2016-003 Text en ©2016 Society for Reproduction and Development http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. |
spellingShingle | Original Article QIU, Lingling XU, Lu CHANG, Guobin GUO, Qixin LIU, Xiangping BI, Yulin ZHANG, Yu WANG, Hongzhi WANG, Kehua LU, Wei REN, Lichen ZHU, Pengfei WU, Yun ZHANG, Yang XU, Qi CHEN, Guohong DNA methylation-mediated transcription factors regulate Piwil1 expression during chicken spermatogenesis |
title | DNA methylation-mediated transcription factors regulate
Piwil1 expression during chicken spermatogenesis |
title_full | DNA methylation-mediated transcription factors regulate
Piwil1 expression during chicken spermatogenesis |
title_fullStr | DNA methylation-mediated transcription factors regulate
Piwil1 expression during chicken spermatogenesis |
title_full_unstemmed | DNA methylation-mediated transcription factors regulate
Piwil1 expression during chicken spermatogenesis |
title_short | DNA methylation-mediated transcription factors regulate
Piwil1 expression during chicken spermatogenesis |
title_sort | dna methylation-mediated transcription factors regulate
piwil1 expression during chicken spermatogenesis |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5004792/ https://www.ncbi.nlm.nih.gov/pubmed/27108736 http://dx.doi.org/10.1262/jrd.2016-003 |
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