Finding New Components of the Mammalian Immune System

The use of forward genetics to analyze mammalian biology has been dramatically accelerated by methods that make it possible instantly to determine which mutation causes a phenotype. Now it is possible to discover gene function as rapidly as mutations can be created and screened: approximately 1,000...

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Detalles Bibliográficos
Autor principal: Beutler, Bruce
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Rambam Health Care Campus 2016
Materias:
Nobel Laureate Perspective
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5005066/
https://www.ncbi.nlm.nih.gov/pubmed/27487306
http://dx.doi.org/10.5041/RMMJ.10245
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author Beutler, Bruce
author_facet Beutler, Bruce
author_sort Beutler, Bruce
collection PubMed
description The use of forward genetics to analyze mammalian biology has been dramatically accelerated by methods that make it possible instantly to determine which mutation causes a phenotype. Now it is possible to discover gene function as rapidly as mutations can be created and screened: approximately 1,000 coding changes per week are interrogated in our laboratory. Moreover, it is possible to know approximately how much damage has been done to the genome over time. We estimate that we have damaged or destroyed about one-quarter of all protein encoding genes and tested the effects of variant alleles within these genes three times or more in a set of phenotypic assays that interest us. Only about two years were required to reach this level of saturation.
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spelling pubmed-50050662016-09-19 Finding New Components of the Mammalian Immune System Beutler, Bruce Rambam Maimonides Med J Nobel Laureate Perspective The use of forward genetics to analyze mammalian biology has been dramatically accelerated by methods that make it possible instantly to determine which mutation causes a phenotype. Now it is possible to discover gene function as rapidly as mutations can be created and screened: approximately 1,000 coding changes per week are interrogated in our laboratory. Moreover, it is possible to know approximately how much damage has been done to the genome over time. We estimate that we have damaged or destroyed about one-quarter of all protein encoding genes and tested the effects of variant alleles within these genes three times or more in a set of phenotypic assays that interest us. Only about two years were required to reach this level of saturation. Rambam Health Care Campus 2016-07-28 /pmc/articles/PMC5005066/ /pubmed/27487306 http://dx.doi.org/10.5041/RMMJ.10245 Text en Copyright: © 2016 Beutler. This is an open-access article. All its content, except where otherwise noted, is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Nobel Laureate Perspective
Beutler, Bruce
Finding New Components of the Mammalian Immune System
title Finding New Components of the Mammalian Immune System
title_full Finding New Components of the Mammalian Immune System
title_fullStr Finding New Components of the Mammalian Immune System
title_full_unstemmed Finding New Components of the Mammalian Immune System
title_short Finding New Components of the Mammalian Immune System
title_sort finding new components of the mammalian immune system
topic Nobel Laureate Perspective
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5005066/
https://www.ncbi.nlm.nih.gov/pubmed/27487306
http://dx.doi.org/10.5041/RMMJ.10245
work_keys_str_mv AT beutlerbruce findingnewcomponentsofthemammalianimmunesystem

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