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Single punch, double biopsy

OBJECTIVE: In lethal primary metastatic prostate cancer, biopsy material is often the only accessible cancer tissue. Lack of tissue quantity limited the use of biopsy cores for analyzing higher numbers of molecular markers and standard histopathologic evaluation for clinical diagnosis simultaneously...

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Autores principales: Krönig, Malte, Nanko, Norbert, Drendel, Vanessa, Werner, Martin, Schultze-Seemann, Wolfgang, Grosu, Anca L., Jilg, A. Cordula
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5005218/
https://www.ncbi.nlm.nih.gov/pubmed/27652032
http://dx.doi.org/10.1186/s40064-016-3141-1
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author Krönig, Malte
Nanko, Norbert
Drendel, Vanessa
Werner, Martin
Schultze-Seemann, Wolfgang
Grosu, Anca L.
Jilg, A. Cordula
author_facet Krönig, Malte
Nanko, Norbert
Drendel, Vanessa
Werner, Martin
Schultze-Seemann, Wolfgang
Grosu, Anca L.
Jilg, A. Cordula
author_sort Krönig, Malte
collection PubMed
description OBJECTIVE: In lethal primary metastatic prostate cancer, biopsy material is often the only accessible cancer tissue. Lack of tissue quantity limited the use of biopsy cores for analyzing higher numbers of molecular markers and standard histopathologic evaluation for clinical diagnosis simultaneously. Recent advances in single cell analytics have paved the way to characterize a tumor in more depth from minute input material such as biopsies. We therefore aimed to develop a biopsy needle, which generates two cores side by side from the same punch: one for standard histopathologic analysis to allow for routine diagnostics and the second one for single cell analytics. METHODS: On the basis of a conventional punch biopsy needle we have milled two parallel longitudinal rifts into the needles shat which are separated by a 100 µm thick metal sheet. Each rift can harbor a single tissue core. RESULTS: Two cores from the same punch were generated reproducibly from a radical prostatectomy specimen and showed congruent results in histopathologic analysis. Both cores yielded equally sufficient material for standard H&E staining and histopathological evaluation. CONCLUSION: Our modified biopsy system will allow for simultaneous acquisition of tissue cores for diagnostic and scientific analysis from solid tumors or metastatic sites.
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spelling pubmed-50052182016-09-20 Single punch, double biopsy Krönig, Malte Nanko, Norbert Drendel, Vanessa Werner, Martin Schultze-Seemann, Wolfgang Grosu, Anca L. Jilg, A. Cordula Springerplus Research OBJECTIVE: In lethal primary metastatic prostate cancer, biopsy material is often the only accessible cancer tissue. Lack of tissue quantity limited the use of biopsy cores for analyzing higher numbers of molecular markers and standard histopathologic evaluation for clinical diagnosis simultaneously. Recent advances in single cell analytics have paved the way to characterize a tumor in more depth from minute input material such as biopsies. We therefore aimed to develop a biopsy needle, which generates two cores side by side from the same punch: one for standard histopathologic analysis to allow for routine diagnostics and the second one for single cell analytics. METHODS: On the basis of a conventional punch biopsy needle we have milled two parallel longitudinal rifts into the needles shat which are separated by a 100 µm thick metal sheet. Each rift can harbor a single tissue core. RESULTS: Two cores from the same punch were generated reproducibly from a radical prostatectomy specimen and showed congruent results in histopathologic analysis. Both cores yielded equally sufficient material for standard H&E staining and histopathological evaluation. CONCLUSION: Our modified biopsy system will allow for simultaneous acquisition of tissue cores for diagnostic and scientific analysis from solid tumors or metastatic sites. Springer International Publishing 2016-08-30 /pmc/articles/PMC5005218/ /pubmed/27652032 http://dx.doi.org/10.1186/s40064-016-3141-1 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Research
Krönig, Malte
Nanko, Norbert
Drendel, Vanessa
Werner, Martin
Schultze-Seemann, Wolfgang
Grosu, Anca L.
Jilg, A. Cordula
Single punch, double biopsy
title Single punch, double biopsy
title_full Single punch, double biopsy
title_fullStr Single punch, double biopsy
title_full_unstemmed Single punch, double biopsy
title_short Single punch, double biopsy
title_sort single punch, double biopsy
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5005218/
https://www.ncbi.nlm.nih.gov/pubmed/27652032
http://dx.doi.org/10.1186/s40064-016-3141-1
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