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Generation of an inducible system to express polo-like kinase, Cdc5 as TAP fusion protein during meiosis in Saccharomyces cerevisiae

Tandem affinity purification (TAP) is a highly efficient method for isolation of protein complexes from endogenous biological macromolecules. TAP system consists of dual affinity tags that facilitates the sequential purification of the desired proteins expressed at their low levels in vivo. Polo-lik...

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Detalles Bibliográficos
Autores principales:	Vaid, Rajni, Dev, Kamal, Lichten, Michael, Sourirajan, Anuradha
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Springer Berlin Heidelberg 2016
Materias:	Protocols and Methods
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5005230/ https://www.ncbi.nlm.nih.gov/pubmed/28330257 http://dx.doi.org/10.1007/s13205-016-0503-x

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