Multicenter Evaluation of the Solana Group A Streptococcus Assay: Comparison with Culture

We compared group A Streptococcus (GAS) culture with a rapid helicase-dependent amplification (HDA) method using 1,082 throat swab specimens. The HDA method demonstrated 98.2% sensitivity and 97.2% specificity. GAS prevalence by culture was 20.7%, and it was 22.6% using the HDA method. In 35 min, th...

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Autores principales: Uphoff, Timothy S., Buchan, Blake W., Ledeboer, Nathan A., Granato, Paul A., Daly, Judy A., Marti, Tara N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2016
Materias:
Bacteriology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5005512/
https://www.ncbi.nlm.nih.gov/pubmed/27358464
http://dx.doi.org/10.1128/JCM.01268-16
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author Uphoff, Timothy S.
Buchan, Blake W.
Ledeboer, Nathan A.
Granato, Paul A.
Daly, Judy A.
Marti, Tara N.
author_facet Uphoff, Timothy S.
Buchan, Blake W.
Ledeboer, Nathan A.
Granato, Paul A.
Daly, Judy A.
Marti, Tara N.
author_sort Uphoff, Timothy S.
collection PubMed
description We compared group A Streptococcus (GAS) culture with a rapid helicase-dependent amplification (HDA) method using 1,082 throat swab specimens. The HDA method demonstrated 98.2% sensitivity and 97.2% specificity. GAS prevalence by culture was 20.7%, and it was 22.6% using the HDA method. In 35 min, the HDA method provided rapid, sensitive GAS detection, making culture confirmation unnecessary.
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spelling pubmed-50055122016-09-14 Multicenter Evaluation of the Solana Group A Streptococcus Assay: Comparison with Culture Uphoff, Timothy S. Buchan, Blake W. Ledeboer, Nathan A. Granato, Paul A. Daly, Judy A. Marti, Tara N. J Clin Microbiol Bacteriology We compared group A Streptococcus (GAS) culture with a rapid helicase-dependent amplification (HDA) method using 1,082 throat swab specimens. The HDA method demonstrated 98.2% sensitivity and 97.2% specificity. GAS prevalence by culture was 20.7%, and it was 22.6% using the HDA method. In 35 min, the HDA method provided rapid, sensitive GAS detection, making culture confirmation unnecessary. American Society for Microbiology 2016-08-24 2016-09 /pmc/articles/PMC5005512/ /pubmed/27358464 http://dx.doi.org/10.1128/JCM.01268-16 Text en Copyright © 2016 Uphoff et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Bacteriology
Uphoff, Timothy S.
Buchan, Blake W.
Ledeboer, Nathan A.
Granato, Paul A.
Daly, Judy A.
Marti, Tara N.
Multicenter Evaluation of the Solana Group A Streptococcus Assay: Comparison with Culture
title Multicenter Evaluation of the Solana Group A Streptococcus Assay: Comparison with Culture
title_full Multicenter Evaluation of the Solana Group A Streptococcus Assay: Comparison with Culture
title_fullStr Multicenter Evaluation of the Solana Group A Streptococcus Assay: Comparison with Culture
title_full_unstemmed Multicenter Evaluation of the Solana Group A Streptococcus Assay: Comparison with Culture
title_short Multicenter Evaluation of the Solana Group A Streptococcus Assay: Comparison with Culture
title_sort multicenter evaluation of the solana group a streptococcus assay: comparison with culture
topic Bacteriology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5005512/
https://www.ncbi.nlm.nih.gov/pubmed/27358464
http://dx.doi.org/10.1128/JCM.01268-16
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