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Creatine Prevents the Structural and Functional Damage to Mitochondria in Myogenic, Oxidatively Stressed C2C12 Cells and Restores Their Differentiation Capacity

Creatine (Cr) is a nutritional supplement promoting a number of health benefits. Indeed Cr has been shown to be beneficial in disease-induced muscle atrophy, improve rehabilitation, and afford mild antioxidant activity. The beneficial effects are likely to derive from pleiotropic interactions. In ac...

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Autores principales: Barbieri, Elena, Guescini, Michele, Calcabrini, Cinzia, Vallorani, Luciana, Diaz, Anna Rita, Fimognari, Carmela, Canonico, Barbara, Luchetti, Francesca, Papa, Stefano, Battistelli, Michela, Falcieri, Elisabetta, Romanello, Vanina, Sandri, Marco, Stocchi, Vilberto, Ciacci, Caterina, Sestili, Piero
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5005540/
https://www.ncbi.nlm.nih.gov/pubmed/27610211
http://dx.doi.org/10.1155/2016/5152029
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author Barbieri, Elena
Guescini, Michele
Calcabrini, Cinzia
Vallorani, Luciana
Diaz, Anna Rita
Fimognari, Carmela
Canonico, Barbara
Luchetti, Francesca
Papa, Stefano
Battistelli, Michela
Falcieri, Elisabetta
Romanello, Vanina
Sandri, Marco
Stocchi, Vilberto
Ciacci, Caterina
Sestili, Piero
author_facet Barbieri, Elena
Guescini, Michele
Calcabrini, Cinzia
Vallorani, Luciana
Diaz, Anna Rita
Fimognari, Carmela
Canonico, Barbara
Luchetti, Francesca
Papa, Stefano
Battistelli, Michela
Falcieri, Elisabetta
Romanello, Vanina
Sandri, Marco
Stocchi, Vilberto
Ciacci, Caterina
Sestili, Piero
author_sort Barbieri, Elena
collection PubMed
description Creatine (Cr) is a nutritional supplement promoting a number of health benefits. Indeed Cr has been shown to be beneficial in disease-induced muscle atrophy, improve rehabilitation, and afford mild antioxidant activity. The beneficial effects are likely to derive from pleiotropic interactions. In accord with this notion, we previously demonstrated that multiple pleiotropic effects, including preservation of mitochondrial damage, account for the capacity of Cr to prevent the differentiation arrest caused by oxidative stress in C2C12 myoblasts. Given the importance of mitochondria in supporting the myogenic process, here we further explored the protective effects of Cr on the structure, function, and networking of these organelles in C2C12 cells differentiating under oxidative stressing conditions; the effects on the energy sensor AMPK, on PGC-1α, which is involved in mitochondrial biogenesis and its downstream effector Tfam were also investigated. Our results indicate that damage to mitochondria is crucial in the differentiation imbalance caused by oxidative stress and that the Cr-prevention of these injuries is invariably associated with the recovery of the normal myogenic capacity. We also found that Cr activates AMPK and induces an upregulation of PGC-1α expression, two events which are likely to contribute to the protection of mitochondrial quality and function.
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spelling pubmed-50055402016-09-08 Creatine Prevents the Structural and Functional Damage to Mitochondria in Myogenic, Oxidatively Stressed C2C12 Cells and Restores Their Differentiation Capacity Barbieri, Elena Guescini, Michele Calcabrini, Cinzia Vallorani, Luciana Diaz, Anna Rita Fimognari, Carmela Canonico, Barbara Luchetti, Francesca Papa, Stefano Battistelli, Michela Falcieri, Elisabetta Romanello, Vanina Sandri, Marco Stocchi, Vilberto Ciacci, Caterina Sestili, Piero Oxid Med Cell Longev Research Article Creatine (Cr) is a nutritional supplement promoting a number of health benefits. Indeed Cr has been shown to be beneficial in disease-induced muscle atrophy, improve rehabilitation, and afford mild antioxidant activity. The beneficial effects are likely to derive from pleiotropic interactions. In accord with this notion, we previously demonstrated that multiple pleiotropic effects, including preservation of mitochondrial damage, account for the capacity of Cr to prevent the differentiation arrest caused by oxidative stress in C2C12 myoblasts. Given the importance of mitochondria in supporting the myogenic process, here we further explored the protective effects of Cr on the structure, function, and networking of these organelles in C2C12 cells differentiating under oxidative stressing conditions; the effects on the energy sensor AMPK, on PGC-1α, which is involved in mitochondrial biogenesis and its downstream effector Tfam were also investigated. Our results indicate that damage to mitochondria is crucial in the differentiation imbalance caused by oxidative stress and that the Cr-prevention of these injuries is invariably associated with the recovery of the normal myogenic capacity. We also found that Cr activates AMPK and induces an upregulation of PGC-1α expression, two events which are likely to contribute to the protection of mitochondrial quality and function. Hindawi Publishing Corporation 2016 2016-08-17 /pmc/articles/PMC5005540/ /pubmed/27610211 http://dx.doi.org/10.1155/2016/5152029 Text en Copyright © 2016 Elena Barbieri et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Barbieri, Elena
Guescini, Michele
Calcabrini, Cinzia
Vallorani, Luciana
Diaz, Anna Rita
Fimognari, Carmela
Canonico, Barbara
Luchetti, Francesca
Papa, Stefano
Battistelli, Michela
Falcieri, Elisabetta
Romanello, Vanina
Sandri, Marco
Stocchi, Vilberto
Ciacci, Caterina
Sestili, Piero
Creatine Prevents the Structural and Functional Damage to Mitochondria in Myogenic, Oxidatively Stressed C2C12 Cells and Restores Their Differentiation Capacity
title Creatine Prevents the Structural and Functional Damage to Mitochondria in Myogenic, Oxidatively Stressed C2C12 Cells and Restores Their Differentiation Capacity
title_full Creatine Prevents the Structural and Functional Damage to Mitochondria in Myogenic, Oxidatively Stressed C2C12 Cells and Restores Their Differentiation Capacity
title_fullStr Creatine Prevents the Structural and Functional Damage to Mitochondria in Myogenic, Oxidatively Stressed C2C12 Cells and Restores Their Differentiation Capacity
title_full_unstemmed Creatine Prevents the Structural and Functional Damage to Mitochondria in Myogenic, Oxidatively Stressed C2C12 Cells and Restores Their Differentiation Capacity
title_short Creatine Prevents the Structural and Functional Damage to Mitochondria in Myogenic, Oxidatively Stressed C2C12 Cells and Restores Their Differentiation Capacity
title_sort creatine prevents the structural and functional damage to mitochondria in myogenic, oxidatively stressed c2c12 cells and restores their differentiation capacity
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5005540/
https://www.ncbi.nlm.nih.gov/pubmed/27610211
http://dx.doi.org/10.1155/2016/5152029
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