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Four-week histologic evaluation of grafted calvarial defects with adjunctive hyperbaric oxygen therapy in rats
PURPOSE: The aim of this study was to characterize the healing in the grafted calvarial defects of rats after adjunctive hyperbaric oxygen therapy. METHODS: Twenty-eight male Sprague-Dawley rats (body weight, 250–300 g) were randomly divided into two treatment groups: with hyperbaric oxygen therapy...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Korean Academy of Periodontology
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5005812/ https://www.ncbi.nlm.nih.gov/pubmed/27588214 http://dx.doi.org/10.5051/jpis.2016.46.4.244 |
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author | Chang, Hyeyoon Oh, Seo-Eun Oh, Seunghan Hu, Kyung-Seok Kim, Sungtae |
author_facet | Chang, Hyeyoon Oh, Seo-Eun Oh, Seunghan Hu, Kyung-Seok Kim, Sungtae |
author_sort | Chang, Hyeyoon |
collection | PubMed |
description | PURPOSE: The aim of this study was to characterize the healing in the grafted calvarial defects of rats after adjunctive hyperbaric oxygen therapy. METHODS: Twenty-eight male Sprague-Dawley rats (body weight, 250–300 g) were randomly divided into two treatment groups: with hyperbaric oxygen therapy (HBO; n=14) and without HBO (NHBO; n=14). Each group was further subdivided according to the bone substitute applied: biphasic calcium phosphate (BCP; n=7) and surface-modified BCP (mBCP; n=7). The mBCP comprised BCP coated with Escherichia-coli-derived recombinant human bone morphogenetic protein-2 (ErhBMP-2) and epigallocatechin-3-gallate (EGCG). Two symmetrical circular defects (6-mm diameter) were created in the right and left parietal bones of each animal. One defect was assigned as a control defect and received no bone substitute, while the other defect was filled with either BCP or mBCP. The animals were allowed to heal for 4 weeks, during which those in the HBO group underwent 5 sessions of HBO. At 4 weeks, the animals were sacrificed, and the defects were harvested for histologic and histomorphometric analysis. RESULTS: Well-maintained space was found in the grafted groups. Woven bone connected to and away from the defect margin was formed. More angiogenesis was found with HBO and EGCG/BMP-2 (P<0.05). None of the defects achieved complete defect closure. Increased new bone formation with HBO or EGCG/BMP-2 was evident in histologic evaluation, but it did not reach statistical significance in histometric analysis. A synergic effect between HBO and EGCG/BMP-2 was not found. CONCLUSIONS: Within the limitations of this study, the present findings indicate that adjunctive HBO and EGCG/BMP-2 could be beneficial for new bone formation in rat calvarial defects. |
format | Online Article Text |
id | pubmed-5005812 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Korean Academy of Periodontology |
record_format | MEDLINE/PubMed |
spelling | pubmed-50058122016-09-01 Four-week histologic evaluation of grafted calvarial defects with adjunctive hyperbaric oxygen therapy in rats Chang, Hyeyoon Oh, Seo-Eun Oh, Seunghan Hu, Kyung-Seok Kim, Sungtae J Periodontal Implant Sci Research Article PURPOSE: The aim of this study was to characterize the healing in the grafted calvarial defects of rats after adjunctive hyperbaric oxygen therapy. METHODS: Twenty-eight male Sprague-Dawley rats (body weight, 250–300 g) were randomly divided into two treatment groups: with hyperbaric oxygen therapy (HBO; n=14) and without HBO (NHBO; n=14). Each group was further subdivided according to the bone substitute applied: biphasic calcium phosphate (BCP; n=7) and surface-modified BCP (mBCP; n=7). The mBCP comprised BCP coated with Escherichia-coli-derived recombinant human bone morphogenetic protein-2 (ErhBMP-2) and epigallocatechin-3-gallate (EGCG). Two symmetrical circular defects (6-mm diameter) were created in the right and left parietal bones of each animal. One defect was assigned as a control defect and received no bone substitute, while the other defect was filled with either BCP or mBCP. The animals were allowed to heal for 4 weeks, during which those in the HBO group underwent 5 sessions of HBO. At 4 weeks, the animals were sacrificed, and the defects were harvested for histologic and histomorphometric analysis. RESULTS: Well-maintained space was found in the grafted groups. Woven bone connected to and away from the defect margin was formed. More angiogenesis was found with HBO and EGCG/BMP-2 (P<0.05). None of the defects achieved complete defect closure. Increased new bone formation with HBO or EGCG/BMP-2 was evident in histologic evaluation, but it did not reach statistical significance in histometric analysis. A synergic effect between HBO and EGCG/BMP-2 was not found. CONCLUSIONS: Within the limitations of this study, the present findings indicate that adjunctive HBO and EGCG/BMP-2 could be beneficial for new bone formation in rat calvarial defects. Korean Academy of Periodontology 2016-08 2016-08-30 /pmc/articles/PMC5005812/ /pubmed/27588214 http://dx.doi.org/10.5051/jpis.2016.46.4.244 Text en Copyright © 2016 Korean Academy of Periodontology http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/). |
spellingShingle | Research Article Chang, Hyeyoon Oh, Seo-Eun Oh, Seunghan Hu, Kyung-Seok Kim, Sungtae Four-week histologic evaluation of grafted calvarial defects with adjunctive hyperbaric oxygen therapy in rats |
title | Four-week histologic evaluation of grafted calvarial defects with adjunctive hyperbaric oxygen therapy in rats |
title_full | Four-week histologic evaluation of grafted calvarial defects with adjunctive hyperbaric oxygen therapy in rats |
title_fullStr | Four-week histologic evaluation of grafted calvarial defects with adjunctive hyperbaric oxygen therapy in rats |
title_full_unstemmed | Four-week histologic evaluation of grafted calvarial defects with adjunctive hyperbaric oxygen therapy in rats |
title_short | Four-week histologic evaluation of grafted calvarial defects with adjunctive hyperbaric oxygen therapy in rats |
title_sort | four-week histologic evaluation of grafted calvarial defects with adjunctive hyperbaric oxygen therapy in rats |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5005812/ https://www.ncbi.nlm.nih.gov/pubmed/27588214 http://dx.doi.org/10.5051/jpis.2016.46.4.244 |
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