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Effects of early feeding on the host rumen transcriptome and bacterial diversity in lambs
Early consumption of starter feed promotes rumen development in lambs. We examined rumen development in lambs fed starter feed for 5 weeks using histological and biochemical analyses and by performing high-throughput sequencing in rumen tissues. Additionally, rumen contents of starter feed-fed lambs...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5006043/ https://www.ncbi.nlm.nih.gov/pubmed/27576848 http://dx.doi.org/10.1038/srep32479 |
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author | Wang, Weimin Li, Chong Li, Fadi Wang, Xiaojuan Zhang, Xiaoxue Liu, Ting Nian, Fang Yue, Xiangpeng Li, Fei Pan, Xiangyu La, Yongfu Mo, Futao Wang, Fangbin Li, Baosheng |
author_facet | Wang, Weimin Li, Chong Li, Fadi Wang, Xiaojuan Zhang, Xiaoxue Liu, Ting Nian, Fang Yue, Xiangpeng Li, Fei Pan, Xiangyu La, Yongfu Mo, Futao Wang, Fangbin Li, Baosheng |
author_sort | Wang, Weimin |
collection | PubMed |
description | Early consumption of starter feed promotes rumen development in lambs. We examined rumen development in lambs fed starter feed for 5 weeks using histological and biochemical analyses and by performing high-throughput sequencing in rumen tissues. Additionally, rumen contents of starter feed-fed lambs were compared to those of breast milk-fed controls. Our physiological and biochemical findings revealed that early starter consumption facilitated rumen development, changed the pattern of ruminal fermentation, and increased the amylase and carboxymethylcellulase activities of rumen micro-organisms. RNA-seq analysis revealed 225 differentially expressed genes between the rumens of breast milk- and starter feed-fed lambs. These DEGs were involved in many metabolic pathways, particularly lipid and carbohydrate metabolism, and included HMGCL and HMGCS2. Sequencing analysis of 16S rRNA genes revealed that ruminal bacterial communities were more diverse in breast milk-than in starter feed-fed lambs, and each group had a distinct microbiota. We conclude that early starter feeding is beneficial to rumen development and physiological function in lambs. The underlying mechanism may involve the stimulation of ruminal ketogenesis and butanoate metabolism via HMGCL and HMGCS2 combined with changes in the fermentation type induced by ruminal microbiota. Overall, this study provides insights into the molecular mechanisms of rumen development in sheep. |
format | Online Article Text |
id | pubmed-5006043 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-50060432016-09-07 Effects of early feeding on the host rumen transcriptome and bacterial diversity in lambs Wang, Weimin Li, Chong Li, Fadi Wang, Xiaojuan Zhang, Xiaoxue Liu, Ting Nian, Fang Yue, Xiangpeng Li, Fei Pan, Xiangyu La, Yongfu Mo, Futao Wang, Fangbin Li, Baosheng Sci Rep Article Early consumption of starter feed promotes rumen development in lambs. We examined rumen development in lambs fed starter feed for 5 weeks using histological and biochemical analyses and by performing high-throughput sequencing in rumen tissues. Additionally, rumen contents of starter feed-fed lambs were compared to those of breast milk-fed controls. Our physiological and biochemical findings revealed that early starter consumption facilitated rumen development, changed the pattern of ruminal fermentation, and increased the amylase and carboxymethylcellulase activities of rumen micro-organisms. RNA-seq analysis revealed 225 differentially expressed genes between the rumens of breast milk- and starter feed-fed lambs. These DEGs were involved in many metabolic pathways, particularly lipid and carbohydrate metabolism, and included HMGCL and HMGCS2. Sequencing analysis of 16S rRNA genes revealed that ruminal bacterial communities were more diverse in breast milk-than in starter feed-fed lambs, and each group had a distinct microbiota. We conclude that early starter feeding is beneficial to rumen development and physiological function in lambs. The underlying mechanism may involve the stimulation of ruminal ketogenesis and butanoate metabolism via HMGCL and HMGCS2 combined with changes in the fermentation type induced by ruminal microbiota. Overall, this study provides insights into the molecular mechanisms of rumen development in sheep. Nature Publishing Group 2016-08-31 /pmc/articles/PMC5006043/ /pubmed/27576848 http://dx.doi.org/10.1038/srep32479 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Wang, Weimin Li, Chong Li, Fadi Wang, Xiaojuan Zhang, Xiaoxue Liu, Ting Nian, Fang Yue, Xiangpeng Li, Fei Pan, Xiangyu La, Yongfu Mo, Futao Wang, Fangbin Li, Baosheng Effects of early feeding on the host rumen transcriptome and bacterial diversity in lambs |
title | Effects of early feeding on the host rumen transcriptome and bacterial diversity in lambs |
title_full | Effects of early feeding on the host rumen transcriptome and bacterial diversity in lambs |
title_fullStr | Effects of early feeding on the host rumen transcriptome and bacterial diversity in lambs |
title_full_unstemmed | Effects of early feeding on the host rumen transcriptome and bacterial diversity in lambs |
title_short | Effects of early feeding on the host rumen transcriptome and bacterial diversity in lambs |
title_sort | effects of early feeding on the host rumen transcriptome and bacterial diversity in lambs |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5006043/ https://www.ncbi.nlm.nih.gov/pubmed/27576848 http://dx.doi.org/10.1038/srep32479 |
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