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Separation and determination of estrogen in the water environment by high performance liquid chromatography-fourier transform infrared spectroscopy

The components for connecting high-performance liquid chromatography (HPLC) with Fourier-transform infrared spectroscopy (FTIR) were investigated to determine estrogen in the water environment, including heating for atomization, solvent removal, sample deposition, drive control, spectrum collection,...

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Autores principales: Zheng, Bei, Li, Wentao, Li, Hongyan, Liu, Lin, Lei, Pei, Ge, Xiaopeng, Yu, Zhiyong, Zhou, Yiqi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5006044/
https://www.ncbi.nlm.nih.gov/pubmed/27577974
http://dx.doi.org/10.1038/srep32264
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author Zheng, Bei
Li, Wentao
Li, Hongyan
Liu, Lin
Lei, Pei
Ge, Xiaopeng
Yu, Zhiyong
Zhou, Yiqi
author_facet Zheng, Bei
Li, Wentao
Li, Hongyan
Liu, Lin
Lei, Pei
Ge, Xiaopeng
Yu, Zhiyong
Zhou, Yiqi
author_sort Zheng, Bei
collection PubMed
description The components for connecting high-performance liquid chromatography (HPLC) with Fourier-transform infrared spectroscopy (FTIR) were investigated to determine estrogen in the water environment, including heating for atomization, solvent removal, sample deposition, drive control, spectrum collection, chip swap, cleaning and drying. Results showed that when the atomization temperature was increased to 388 K, the interference of mobile phase components (methanol, H(2)O, acetonitrile, and NaH(2)PO(4)) were completely removed in the IR measurement of estrogen, with 0.999 of similarity between IR spectra obtained after separation and corresponding to the standard IR spectra. In experiments with varying HPLC injection volumes, high similarity for IR spectra was obtained at 20 ul injection volume at 0.01 mg/L BPA while a useful IR spectrum for 10 ng/L BPA was obtained at 80 ul injection volume. In addition, estrogen concentrations in the natural water samples were calculated semi-quantitatively from the peak intensities of IR spectrum in the mid-infrared region.
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spelling pubmed-50060442016-09-07 Separation and determination of estrogen in the water environment by high performance liquid chromatography-fourier transform infrared spectroscopy Zheng, Bei Li, Wentao Li, Hongyan Liu, Lin Lei, Pei Ge, Xiaopeng Yu, Zhiyong Zhou, Yiqi Sci Rep Article The components for connecting high-performance liquid chromatography (HPLC) with Fourier-transform infrared spectroscopy (FTIR) were investigated to determine estrogen in the water environment, including heating for atomization, solvent removal, sample deposition, drive control, spectrum collection, chip swap, cleaning and drying. Results showed that when the atomization temperature was increased to 388 K, the interference of mobile phase components (methanol, H(2)O, acetonitrile, and NaH(2)PO(4)) were completely removed in the IR measurement of estrogen, with 0.999 of similarity between IR spectra obtained after separation and corresponding to the standard IR spectra. In experiments with varying HPLC injection volumes, high similarity for IR spectra was obtained at 20 ul injection volume at 0.01 mg/L BPA while a useful IR spectrum for 10 ng/L BPA was obtained at 80 ul injection volume. In addition, estrogen concentrations in the natural water samples were calculated semi-quantitatively from the peak intensities of IR spectrum in the mid-infrared region. Nature Publishing Group 2016-08-31 /pmc/articles/PMC5006044/ /pubmed/27577974 http://dx.doi.org/10.1038/srep32264 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Zheng, Bei
Li, Wentao
Li, Hongyan
Liu, Lin
Lei, Pei
Ge, Xiaopeng
Yu, Zhiyong
Zhou, Yiqi
Separation and determination of estrogen in the water environment by high performance liquid chromatography-fourier transform infrared spectroscopy
title Separation and determination of estrogen in the water environment by high performance liquid chromatography-fourier transform infrared spectroscopy
title_full Separation and determination of estrogen in the water environment by high performance liquid chromatography-fourier transform infrared spectroscopy
title_fullStr Separation and determination of estrogen in the water environment by high performance liquid chromatography-fourier transform infrared spectroscopy
title_full_unstemmed Separation and determination of estrogen in the water environment by high performance liquid chromatography-fourier transform infrared spectroscopy
title_short Separation and determination of estrogen in the water environment by high performance liquid chromatography-fourier transform infrared spectroscopy
title_sort separation and determination of estrogen in the water environment by high performance liquid chromatography-fourier transform infrared spectroscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5006044/
https://www.ncbi.nlm.nih.gov/pubmed/27577974
http://dx.doi.org/10.1038/srep32264
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