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Over-expression of Topoisomerase II Enhances Salt Stress Tolerance in Tobacco
Topoisomerases are unique enzymes having an ability to remove or add DNA supercoils and untangle the snarled DNA. They can cut, shuffle, and religate DNA strands and remove the torsional stress during DNA replication, transcription or recombination events. In the present study, we over-expressed top...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5006100/ https://www.ncbi.nlm.nih.gov/pubmed/27630644 http://dx.doi.org/10.3389/fpls.2016.01280 |
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author | John, Riffat Ganeshan, Uma Singh, Badri N. Kaul, Tanushri Reddy, Malireddy K. Sopory, Sudhir K. Rajam, Manchikatla V. |
author_facet | John, Riffat Ganeshan, Uma Singh, Badri N. Kaul, Tanushri Reddy, Malireddy K. Sopory, Sudhir K. Rajam, Manchikatla V. |
author_sort | John, Riffat |
collection | PubMed |
description | Topoisomerases are unique enzymes having an ability to remove or add DNA supercoils and untangle the snarled DNA. They can cut, shuffle, and religate DNA strands and remove the torsional stress during DNA replication, transcription or recombination events. In the present study, we over-expressed topoisomerase II (TopoII) in tobacco (Nicotiana tabaccum) and examined its role in growth and development as well as salt (NaCl) stress tolerance. Several putative transgenic plants were generated and the transgene integration and expression was confirmed by PCR and Southern blot analyses, and RT-PCR analysis respectively. Percent seed germination, shoot growth, and chlorophyll content revealed that transgenic lines over-expressing the NtTopoIIα-1 gene exhibited enhanced tolerance to salt (150 and 200 mM NaCl) stress. Moreover, over-expression of TopoII lead to the elevation in proline and glycine betaine levels in response to both concentrations of NaCl as compared to wild-type. In response to NaCl stress, TopoII over-expressing lines showed reduced lipid peroxidation derived malondialdehyde (MDA) generation. These results suggest that TopoII plays a pivotal role in salt stress tolerance in plants. |
format | Online Article Text |
id | pubmed-5006100 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-50061002016-09-14 Over-expression of Topoisomerase II Enhances Salt Stress Tolerance in Tobacco John, Riffat Ganeshan, Uma Singh, Badri N. Kaul, Tanushri Reddy, Malireddy K. Sopory, Sudhir K. Rajam, Manchikatla V. Front Plant Sci Plant Science Topoisomerases are unique enzymes having an ability to remove or add DNA supercoils and untangle the snarled DNA. They can cut, shuffle, and religate DNA strands and remove the torsional stress during DNA replication, transcription or recombination events. In the present study, we over-expressed topoisomerase II (TopoII) in tobacco (Nicotiana tabaccum) and examined its role in growth and development as well as salt (NaCl) stress tolerance. Several putative transgenic plants were generated and the transgene integration and expression was confirmed by PCR and Southern blot analyses, and RT-PCR analysis respectively. Percent seed germination, shoot growth, and chlorophyll content revealed that transgenic lines over-expressing the NtTopoIIα-1 gene exhibited enhanced tolerance to salt (150 and 200 mM NaCl) stress. Moreover, over-expression of TopoII lead to the elevation in proline and glycine betaine levels in response to both concentrations of NaCl as compared to wild-type. In response to NaCl stress, TopoII over-expressing lines showed reduced lipid peroxidation derived malondialdehyde (MDA) generation. These results suggest that TopoII plays a pivotal role in salt stress tolerance in plants. Frontiers Media S.A. 2016-08-31 /pmc/articles/PMC5006100/ /pubmed/27630644 http://dx.doi.org/10.3389/fpls.2016.01280 Text en Copyright © 2016 John, Ganeshan, Singh, Kaul, Reddy, Sopory and Rajam. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science John, Riffat Ganeshan, Uma Singh, Badri N. Kaul, Tanushri Reddy, Malireddy K. Sopory, Sudhir K. Rajam, Manchikatla V. Over-expression of Topoisomerase II Enhances Salt Stress Tolerance in Tobacco |
title | Over-expression of Topoisomerase II Enhances Salt Stress Tolerance in Tobacco |
title_full | Over-expression of Topoisomerase II Enhances Salt Stress Tolerance in Tobacco |
title_fullStr | Over-expression of Topoisomerase II Enhances Salt Stress Tolerance in Tobacco |
title_full_unstemmed | Over-expression of Topoisomerase II Enhances Salt Stress Tolerance in Tobacco |
title_short | Over-expression of Topoisomerase II Enhances Salt Stress Tolerance in Tobacco |
title_sort | over-expression of topoisomerase ii enhances salt stress tolerance in tobacco |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5006100/ https://www.ncbi.nlm.nih.gov/pubmed/27630644 http://dx.doi.org/10.3389/fpls.2016.01280 |
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