Cargando…

Efficient Inactivation of Symbiotic Nitrogen Fixation Related Genes in Lotus japonicus Using CRISPR-Cas9

The targeted genome editing technique, CRISPR/Cas9 system, has been widely used to modify genes of interest in a predictable and precise manner. In this study, we describe the CRISPR/Cas9-mediated efficient editing of representative SNF (symbiotic nitrogen fixation) related genes in the model legume...

Descripción completa

Detalles Bibliográficos
Autores principales:	Wang, Longxiang, Wang, Longlong, Tan, Qian, Fan, Qiuling, Zhu, Hui, Hong, Zonglie, Zhang, Zhongming, Duanmu, Deqiang
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2016
Materias:	Plant Science
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5006320/ https://www.ncbi.nlm.nih.gov/pubmed/27630657 http://dx.doi.org/10.3389/fpls.2016.01333

Ejemplares similares

Molecular Characterization of Carbonic Anhydrase Genes in Lotus japonicus and Their Potential Roles in Symbiotic Nitrogen Fixation
por: Wang, Longlong, et al.
Publicado: (2021)

Lotus japonicus alters in planta fitness of Mesorhizobium loti dependent on symbiotic nitrogen fixation
por: Quides, Kenjiro W., et al.
Publicado: (2017)

Dephosphorylation of LjMPK6 by Phosphatase LjPP2C is Involved in Regulating Nodule Organogenesis in Lotus japonicus
por: Yan, Zhongyuan, et al.
Publicado: (2020)

Early Lotus japonicus root transcriptomic responses to symbiotic and pathogenic fungal exudates
por: Giovannetti, Marco, et al.
Publicado: (2015)

Epidermal LysM receptor ensures robust symbiotic signalling in Lotus japonicus
por: Murakami, Eiichi, et al.
Publicado: (2018)

The Lotus japonicus Ubiquitin Ligase SIE3 Interacts With the Transcription Factor SIP1 and Forms a Homodimer
por: Feng, Yong, et al.
Publicado: (2020)

Genome Editing in Cowpea Vigna unguiculata Using CRISPR-Cas9
por: Ji, Jie, et al.
Publicado: (2019)

Distinct Lotus japonicus Transcriptomic Responses to a Spectrum of Bacteria Ranging From Symbiotic to Pathogenic
por: Kelly, Simon, et al.
Publicado: (2018)

Characterization of the Heme Pocket Structure and Ligand Binding Kinetics of Non-symbiotic Hemoglobins from the Model Legume Lotus japonicus
por: Calvo-Begueria, Laura, et al.
Publicado: (2017)

The Lotus japonicus ROP3 Is Involved in the Establishment of the Nitrogen-Fixing Symbiosis but Not of the Arbuscular Mycorrhizal Symbiosis
por: García-Soto, Ivette, et al.
Publicado: (2021)

Transcriptome Response Mediated by Cold Stress in Lotus japonicus
por: Calzadilla, Pablo I., et al.
Publicado: (2016)

Domain Swap Approach Reveals the Critical Roles of Different Domains of SYMRK in Root Nodule Symbiosis in Lotus japonicus
por: Li, Hao, et al.
Publicado: (2018)

Ectopic Expression of PII Induces Stomatal Closure in Lotus japonicus
por: Parlati, Aurora, et al.
Publicado: (2017)

Fixating on metals: new insights into the role of metals in nodulation and symbiotic nitrogen fixation
por: González-Guerrero, Manuel, et al.
Publicado: (2014)

Three classes of hemoglobins are required for optimal vegetative and reproductive growth of Lotus japonicus: genetic and biochemical characterization of LjGlb2-1
por: Villar, Irene, et al.
Publicado: (2021)

ARBUSCULAR MYCORRHIZA-INDUCED KINASES AMK8 and AMK24 associate with the receptor-like kinase KINASE3 to regulate arbuscular mycorrhizal symbiosis in Lotus japonicus
por: Leng, Junchen, et al.
Publicado: (2023)

Aromatic amino acid biosynthesis impacts root hair development and symbiotic associations in Lotus japonicus
por: Montiel, Jesús, et al.
Publicado: (2023)

MtCAS31 Aids Symbiotic Nitrogen Fixation by Protecting the Leghemoglobin MtLb120-1 Under Drought Stress in Medicago truncatula
por: Li, Xin, et al.
Publicado: (2018)

Delayed maturation of nodules reduces symbiotic effectiveness of the Lotus japonicus–Rhizobium sp. NGR234 interaction
por: Schumpp, Olivier, et al.
Publicado: (2009)

Nicotianamine Synthase 2 Is Required for Symbiotic Nitrogen Fixation in Medicago truncatula Nodules
por: Escudero, Viviana, et al.
Publicado: (2020)

Editorial: Nutrient Dependent Signaling Pathways Controlling the Symbiotic Nitrogen Fixation Process
por: Suzaki, Takuya, et al.
Publicado: (2021)

Identification of loci associated with water use efficiency and symbiotic nitrogen fixation in soybean
por: Arifuzzaman, Muhammad, et al.
Publicado: (2023)

Biological and genomic analysis of a symbiotic nitrogen fixation defective mutant in Medicago truncatula
por: Shen, Yitong, et al.
Publicado: (2023)

The effects of ERN1 on gene expression during early rhizobial infection in Lotus japonicus
por: Liu, Meng, et al.
Publicado: (2023)

Symbiotic responses of Lotus japonicus to two isogenic lines of a mycorrhizal fungus differing in the presence/absence of an endobacterium
por: Venice, Francesco, et al.
Publicado: (2021)

A glycan receptor kinase facilitates intracellular accommodation of arbuscular mycorrhiza and symbiotic rhizobia in the legume Lotus japonicus
por: Kelly, Simon, et al.
Publicado: (2023)

The WRKY Transcription Factor Genes in Lotus japonicus
por: Song, Hui, et al.
Publicado: (2014)

Highly Efficient CRISPR-Mediated Base Editing in Sinorhizobium meliloti
por: Wang, Longxiang, et al.
Publicado: (2021)

Single cell‐type transcriptome profiling reveals genes that promote nitrogen fixation in the infected and uninfected cells of legume nodules
por: Wang, Longlong, et al.
Publicado: (2022)

Editorial: Metabolic Adjustments and Gene Expression Reprogramming for Symbiotic Nitrogen Fixation in Legume Nodules
por: Ferguson, Brett James, et al.
Publicado: (2019)

Genome Structure of the Legume, Lotus japonicus
por: Sato, Shusei, et al.
Publicado: (2008)

Lotus Base: An integrated information portal for the model legume Lotus japonicus
por: Mun, Terry, et al.
Publicado: (2016)

Flavonoids and Isoflavonoids Biosynthesis in the Model Legume Lotus japonicus; Connections to Nitrogen Metabolism and Photorespiration
por: García-Calderón, Margarita, et al.
Publicado: (2020)

AP2 transcription factor CBX1 with a specific function in symbiotic exchange of nutrients in mycorrhizal Lotus japonicus
por: Xue, Li, et al.
Publicado: (2018)

Editorial: Nutrient dependent signaling pathways controlling the symbiotic nitrogen fixation process, Volume II
por: Valkov, Vladimir Totev, et al.
Publicado: (2023)

Genetic diversity and symbiotic compatibility among rhizobial strains and Desmodium incanum and Lotus spp. plants
por: Granada, Camille E., et al.
Publicado: (2014)

Mutants of Lotus japonicus deficient in flavonoid biosynthesis
por: Aoki, Toshio, et al.
Publicado: (2021)

Editorial: Metabolic adjustments and gene expression reprogramming for symbiotic nitrogen fixation in legume nodules, volume II
por: Fan, Kejing, et al.
Publicado: (2023)

Medicago truncatula PHO2 genes have distinct roles in phosphorus homeostasis and symbiotic nitrogen fixation
por: Huertas, Raul, et al.
Publicado: (2023)

Modulation of phenolic metabolism under stress conditions in a Lotus japonicus mutant lacking plastidic glutamine synthetase
por: García-Calderón, Margarita, et al.
Publicado: (2015)

Cannot write session to /tmp/vufind_sessions/sess_06ljtfss6mjelqhcg2gt68m0fs