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Improving the efficiency of molecular replacement by utilizing a new iterative transform phasing algorithm

An iterative transform method proposed previously for direct phasing of high-solvent-content protein crystals is employed for enhancing the molecular-replacement (MR) algorithm in protein crystallography. Target structures that are resistant to conventional MR due to insufficient similarity between...

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Detalles Bibliográficos
Autores principales: He, Hongxing, Fang, Hengrui, Miller, Mitchell D., Phillips, George N., Su, Wu-Pei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5006650/
https://www.ncbi.nlm.nih.gov/pubmed/27580202
http://dx.doi.org/10.1107/S2053273316010731
Descripción
Sumario:An iterative transform method proposed previously for direct phasing of high-solvent-content protein crystals is employed for enhancing the molecular-replacement (MR) algorithm in protein crystallography. Target structures that are resistant to conventional MR due to insufficient similarity between the template and target structures might be tractable with this modified phasing method. Trial calculations involving three different structures are described to test and illustrate the methodology. The relationship of the approach to PHENIX Phaser-MR and MR-Rosetta is discussed.