Improving the efficiency of molecular replacement by utilizing a new iterative transform phasing algorithm

An iterative transform method proposed previously for direct phasing of high-solvent-content protein crystals is employed for enhancing the molecular-replacement (MR) algorithm in protein crystallography. Target structures that are resistant to conventional MR due to insufficient similarity between...

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Detalles Bibliográficos
Autores principales: He, Hongxing, Fang, Hengrui, Miller, Mitchell D., Phillips, George N., Su, Wu-Pei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2016
Materias:
Research Papers
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5006650/
https://www.ncbi.nlm.nih.gov/pubmed/27580202
http://dx.doi.org/10.1107/S2053273316010731
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author He, Hongxing
Fang, Hengrui
Miller, Mitchell D.
Phillips, George N.
Su, Wu-Pei
author_facet He, Hongxing
Fang, Hengrui
Miller, Mitchell D.
Phillips, George N.
Su, Wu-Pei
author_sort He, Hongxing
collection PubMed
description An iterative transform method proposed previously for direct phasing of high-solvent-content protein crystals is employed for enhancing the molecular-replacement (MR) algorithm in protein crystallography. Target structures that are resistant to conventional MR due to insufficient similarity between the template and target structures might be tractable with this modified phasing method. Trial calculations involving three different structures are described to test and illustrate the methodology. The relationship of the approach to PHENIX Phaser-MR and MR-Rosetta is discussed.
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spelling pubmed-50066502016-09-14 Improving the efficiency of molecular replacement by utilizing a new iterative transform phasing algorithm He, Hongxing Fang, Hengrui Miller, Mitchell D. Phillips, George N. Su, Wu-Pei Acta Crystallogr A Found Adv Research Papers An iterative transform method proposed previously for direct phasing of high-solvent-content protein crystals is employed for enhancing the molecular-replacement (MR) algorithm in protein crystallography. Target structures that are resistant to conventional MR due to insufficient similarity between the template and target structures might be tractable with this modified phasing method. Trial calculations involving three different structures are described to test and illustrate the methodology. The relationship of the approach to PHENIX Phaser-MR and MR-Rosetta is discussed. International Union of Crystallography 2016-07-15 /pmc/articles/PMC5006650/ /pubmed/27580202 http://dx.doi.org/10.1107/S2053273316010731 Text en © Hongxing He et al. 2016 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
spellingShingle Research Papers
He, Hongxing
Fang, Hengrui
Miller, Mitchell D.
Phillips, George N.
Su, Wu-Pei
Improving the efficiency of molecular replacement by utilizing a new iterative transform phasing algorithm
title Improving the efficiency of molecular replacement by utilizing a new iterative transform phasing algorithm
title_full Improving the efficiency of molecular replacement by utilizing a new iterative transform phasing algorithm
title_fullStr Improving the efficiency of molecular replacement by utilizing a new iterative transform phasing algorithm
title_full_unstemmed Improving the efficiency of molecular replacement by utilizing a new iterative transform phasing algorithm
title_short Improving the efficiency of molecular replacement by utilizing a new iterative transform phasing algorithm
title_sort improving the efficiency of molecular replacement by utilizing a new iterative transform phasing algorithm
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5006650/
https://www.ncbi.nlm.nih.gov/pubmed/27580202
http://dx.doi.org/10.1107/S2053273316010731
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