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Cytokinin-Regulated Sucrose Metabolism in Stay-Green Wheat Phenotype

A wheat stay-green mutant, tasg1, was observed to exhibit significantly delayed senescence in the late developmental stage. The photosynthetic capacity of the flag leaf was greater in tasg1 than in wild type (WT) plants. In addition, the grain volume of tasg1 was significantly higher than that of WT...

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Autores principales: Wang, Wenqiang, Hao, Qunqun, Tian, Fengxia, Li, Qinxue, Wang, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5007033/
https://www.ncbi.nlm.nih.gov/pubmed/27580166
http://dx.doi.org/10.1371/journal.pone.0161351
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author Wang, Wenqiang
Hao, Qunqun
Tian, Fengxia
Li, Qinxue
Wang, Wei
author_facet Wang, Wenqiang
Hao, Qunqun
Tian, Fengxia
Li, Qinxue
Wang, Wei
author_sort Wang, Wenqiang
collection PubMed
description A wheat stay-green mutant, tasg1, was observed to exhibit significantly delayed senescence in the late developmental stage. The photosynthetic capacity of the flag leaf was greater in tasg1 than in wild type (WT) plants. In addition, the grain volume of tasg1 was significantly higher than that of WT at the early filling stage. The content of various cytokinins (CKs) in the grain was significantly higher in tasg1 than in WT and was accompanied by an upregulated expression of some cell cycle-related genes. Examination of the metabolism of soluble sugars in tasg1 and WT revealed that the concentrations of glucose (Glu), fructose (Fru), and sucrose (Suc) were higher in the flag leaves and grains of tasg1 than in WT plants. The activities of sucrose-phosphate synthase (SPS), sucrose synthase (SuSy), and cell wall invertase (CW-invertase) were higher in tasg1, suggesting an altered metabolism and transport of soluble sugars. Furthermore, when tasg1 was treated with the CK inhibitor lovastatin, the activity of invertase was inhibited and was associated with premature senescence phenotype. However, the activity of invertase was partially recovered in tasg1 when treated with 6-benzylaminopurine (BAP). The trend of change in the concentrations of Glu, Fru, and Suc was similar to that of invertase. Our results suggest that CKs might regulate the stay-green phenotype of tasg1 by regulating the invertase activity involved in Suc remobilization.
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spelling pubmed-50070332016-09-27 Cytokinin-Regulated Sucrose Metabolism in Stay-Green Wheat Phenotype Wang, Wenqiang Hao, Qunqun Tian, Fengxia Li, Qinxue Wang, Wei PLoS One Research Article A wheat stay-green mutant, tasg1, was observed to exhibit significantly delayed senescence in the late developmental stage. The photosynthetic capacity of the flag leaf was greater in tasg1 than in wild type (WT) plants. In addition, the grain volume of tasg1 was significantly higher than that of WT at the early filling stage. The content of various cytokinins (CKs) in the grain was significantly higher in tasg1 than in WT and was accompanied by an upregulated expression of some cell cycle-related genes. Examination of the metabolism of soluble sugars in tasg1 and WT revealed that the concentrations of glucose (Glu), fructose (Fru), and sucrose (Suc) were higher in the flag leaves and grains of tasg1 than in WT plants. The activities of sucrose-phosphate synthase (SPS), sucrose synthase (SuSy), and cell wall invertase (CW-invertase) were higher in tasg1, suggesting an altered metabolism and transport of soluble sugars. Furthermore, when tasg1 was treated with the CK inhibitor lovastatin, the activity of invertase was inhibited and was associated with premature senescence phenotype. However, the activity of invertase was partially recovered in tasg1 when treated with 6-benzylaminopurine (BAP). The trend of change in the concentrations of Glu, Fru, and Suc was similar to that of invertase. Our results suggest that CKs might regulate the stay-green phenotype of tasg1 by regulating the invertase activity involved in Suc remobilization. Public Library of Science 2016-08-31 /pmc/articles/PMC5007033/ /pubmed/27580166 http://dx.doi.org/10.1371/journal.pone.0161351 Text en © 2016 Wang et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Wang, Wenqiang
Hao, Qunqun
Tian, Fengxia
Li, Qinxue
Wang, Wei
Cytokinin-Regulated Sucrose Metabolism in Stay-Green Wheat Phenotype
title Cytokinin-Regulated Sucrose Metabolism in Stay-Green Wheat Phenotype
title_full Cytokinin-Regulated Sucrose Metabolism in Stay-Green Wheat Phenotype
title_fullStr Cytokinin-Regulated Sucrose Metabolism in Stay-Green Wheat Phenotype
title_full_unstemmed Cytokinin-Regulated Sucrose Metabolism in Stay-Green Wheat Phenotype
title_short Cytokinin-Regulated Sucrose Metabolism in Stay-Green Wheat Phenotype
title_sort cytokinin-regulated sucrose metabolism in stay-green wheat phenotype
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5007033/
https://www.ncbi.nlm.nih.gov/pubmed/27580166
http://dx.doi.org/10.1371/journal.pone.0161351
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AT liqinxue cytokininregulatedsucrosemetabolisminstaygreenwheatphenotype
AT wangwei cytokininregulatedsucrosemetabolisminstaygreenwheatphenotype