Efficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA

Editing plant genomes is technically challenging in hard-to-transform plants and usually involves transgenic intermediates, which causes regulatory concerns. Here we report two simple and efficient genome-editing methods in which plants are regenerated from callus cells transiently expressing CRISPR...

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Detalles Bibliográficos
Autores principales: Zhang, Yi, Liang, Zhen, Zong, Yuan, Wang, Yanpeng, Liu, Jinxing, Chen, Kunling, Qiu, Jin-Long, Gao, Caixia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5007326/
https://www.ncbi.nlm.nih.gov/pubmed/27558837
http://dx.doi.org/10.1038/ncomms12617

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