Cargando…

Asymmetric cryo-EM reconstruction of phage MS2 reveals genome structure in situ

In single-stranded ribonucleic acid (RNA) viruses, virus capsid assembly and genome packaging are intertwined processes. Using cryo-electron microscopy and single particle analysis we determined the asymmetric virion structure of bacteriophage MS2, which includes 178 copies of the coat protein, a si...

Descripción completa

Detalles Bibliográficos
Autores principales:	Koning, Roman I, Gomez-Blanco, Josue, Akopjana, Inara, Vargas, Javier, Kazaks, Andris, Tars, Kaspars, Carazo, José María, Koster, Abraham J.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group 2016
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5007439/ https://www.ncbi.nlm.nih.gov/pubmed/27561669 http://dx.doi.org/10.1038/ncomms12524

Ejemplares similares

Crystal structure of the membrane attack complex assembly inhibitor BGA71 from the Lyme disease agent Borrelia bavariensis
por: Brangulis, Kalvis, et al.
Publicado: (2018)

Structural Basis for DNA Recognition of a Single-stranded DNA-binding Protein from Enterobacter Phage Enc34
por: Cernooka, Elina, et al.
Publicado: (2017)

Diversity of the lysozyme fold: structure of the catalytic domain from an unusual endolysin encoded by phage Enc34
por: Cernooka, Elina, et al.
Publicado: (2022)

Construction and Immunogenicity of a Novel Multivalent Vaccine Prototype Based on Conserved Influenza Virus Antigens
por: Kirsteina, Anna, et al.
Publicado: (2020)

Three-dimensional structure of 22 uncultured ssRNA bacteriophages: Flexibility of the coat protein fold and variations in particle shapes
por: Rūmnieks, Jānis, et al.
Publicado: (2020)

ssRNA Phages: Life Cycle, Structure and Applications
por: Tars, Kaspars
Publicado: (2020)

Production and characterization of novel ssRNA bacteriophage virus-like particles from metagenomic sequencing data
por: Liekniņa, Ilva, et al.
Publicado: (2019)

DeepEMhancer: a deep learning solution for cryo-EM volume post-processing
por: Sanchez-Garcia, Ruben, et al.
Publicado: (2021)

Diversity of pili-specific bacteriophages: genome sequence of IncM plasmid-dependent RNA phage M
por: Rumnieks, Janis, et al.
Publicado: (2012)

Structure and applications of novel influenza HA tri-stalk protein for evaluation of HA stem-specific immunity
por: Lu, I-Na, et al.
Publicado: (2018)

3H-1,2-benzoxathiepine 2,2-dioxides: a new class of isoform-selective carbonic anhydrase inhibitors
por: Pustenko, Aleksandrs, et al.
Publicado: (2017)

Novel Erwinia persicina Infecting Phage Midgardsormr38 Within the Context of Temperate Erwinia Phages
por: Zrelovs, Nikita, et al.
Publicado: (2020)

Cryo-EM reveals the asymmetric assembly of squid hemocyanin
por: Tanaka, Yoshikazu, et al.
Publicado: (2019)

Motley Crew: Overview of the Currently Available Phage Diversity
por: Zrelovs, Nikita, et al.
Publicado: (2020)

Measuring local-directional resolution and local anisotropy in cryo-EM maps
por: Vilas, Jose Luis, et al.
Publicado: (2020)

Corrigendum: Motley Crew: Overview of the Currently Available Phage Diversity
por: Zrelovs, Nikita, et al.
Publicado: (2021)

In situ structure and dynamics of an alphacoronavirus spike protein by cryo-ET and cryo-EM
por: Huang, Cheng-Yu, et al.
Publicado: (2022)

Three Phages One Host: Isolation and Characterization of Pantoea agglomerans Phages from a Grasshopper Specimen
por: Zrelovs, Nikita, et al.
Publicado: (2023)

Expression and purification of phage T7 ejection proteins for cryo-EM analysis
por: Swanson, Nicholas A., et al.
Publicado: (2021)

Cryo-EM structure of the RADAR supramolecular anti-phage defense complex
por: Duncan-Lowey, Brianna, et al.
Publicado: (2023)

Production and purification of chimeric HBc virus-like particles carrying influenza virus LAH domain as vaccine candidates
por: Kazaks, Andris, et al.
Publicado: (2017)

Principal component analysis is limited to low-resolution analysis in cryoEM
por: Sorzano, Carlos Oscar S., et al.
Publicado: (2021)

Local computational methods to improve the interpretability and analysis of cryo-EM maps
por: Kaur, Satinder, et al.
Publicado: (2021)

Genome Characterization of Nocturne116, Novel Lactococcus lactis-Infecting Phage Isolated from Moth
por: Zrelovs, Nikita, et al.
Publicado: (2021)

Aryl-4,5-dihydro-1H-pyrazole-1-carboxamide Derivatives Bearing a Sulfonamide Moiety Show Single-digit Nanomolar-to-Subnanomolar Inhibition Constants against the Tumor-associated Human Carbonic Anhydrases IX and XII
por: Hargunani, Priya, et al.
Publicado: (2020)

Emerging Themes in CryoEM—Single Particle Analysis Image Processing
por: Vilas, Jose Luis, et al.
Publicado: (2022)

Extending the Inhibition Profiles of Coumarin-Based Compounds Against Human Carbonic Anhydrases: Synthesis, Biological, and In Silico Evaluation
por: Kartsev, Victor, et al.
Publicado: (2019)

Automatic local resolution-based sharpening of cryo-EM maps
por: Ramírez-Aportela, Erney, et al.
Publicado: (2019)

Higher resolution in cryo-EM by the combination of macromolecular prior knowledge and image-processing tools
por: Ramírez-Aportela, Erney, et al.
Publicado: (2022)

Automated vitrification of cryo-EM samples with controllable sample thickness using suction and real-time optical inspection
por: Koning, Roman I., et al.
Publicado: (2022)

Morganella Phage Mecenats66 Utilizes an Evolutionarily Distinct Subtype of Headful Genome Packaging with a Preferred Packaging Initiation Site
por: Zrelovs, Nikita, et al.
Publicado: (2022)

Cryo-EM and the elucidation of new macromolecular structures: Random Conical Tilt revisited
por: Sorzano, C. O. S., et al.
Publicado: (2015)

Cryo-EM Structures of Two Bacteriophage Portal Proteins Provide Insights for Antimicrobial Phage Engineering
por: Javed, Abid, et al.
Publicado: (2021)

Structural Analysis of the Outer Membrane Lipoprotein BBA14 (OrfD) and the Corresponding Paralogous Gene Family 143 (PFam143) from Borrelia burgdorferi
por: Akopjana, Inara, et al.
Publicado: (2022)

Using Scipion for stream image processing at Cryo-EM facilities
por: Gómez-Blanco, J., et al.
Publicado: (2018)

In situ cryo-electron tomography reveals the asymmetric architecture of mammalian sperm axonemes
por: Chen, Zhen, et al.
Publicado: (2023)

A snapshot of cryo‐EM
por: Skiniotis, Georgios
Publicado: (2016)

Interpreting the cryo-EM map
por: Rosenthal, Peter B.
Publicado: (2019)

COVID-19 and cryo-EM
por: Subramaniam, Sriram
Publicado: (2020)

Cryo-EM at ACA 2022
por: Subramaniam, Sriram, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_rdga4uco1bieh01050b55pacit