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CRISPR-Cas9 enables conditional mutagenesis of challenging loci

The International Knockout Mouse Consortium (IKMC) has produced a genome-wide collection of 15,000 isogenic targeting vectors for conditional mutagenesis in C57BL/6N mice. Although most of the vectors have been used successfully in murine embryonic stem (ES) cells, there remain a set of nearly two t...

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Autores principales: Schick, Joel A., Seisenberger, Claudia, Beig, Joachim, Bürger, Antje, Iyer, Vivek, Maier, Viola, Perera, Sajith, Rosen, Barry, Skarnes, William C., Wurst, Wolfgang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5007477/
https://www.ncbi.nlm.nih.gov/pubmed/27580957
http://dx.doi.org/10.1038/srep32326
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author Schick, Joel A.
Seisenberger, Claudia
Beig, Joachim
Bürger, Antje
Iyer, Vivek
Maier, Viola
Perera, Sajith
Rosen, Barry
Skarnes, William C.
Wurst, Wolfgang
author_facet Schick, Joel A.
Seisenberger, Claudia
Beig, Joachim
Bürger, Antje
Iyer, Vivek
Maier, Viola
Perera, Sajith
Rosen, Barry
Skarnes, William C.
Wurst, Wolfgang
author_sort Schick, Joel A.
collection PubMed
description The International Knockout Mouse Consortium (IKMC) has produced a genome-wide collection of 15,000 isogenic targeting vectors for conditional mutagenesis in C57BL/6N mice. Although most of the vectors have been used successfully in murine embryonic stem (ES) cells, there remain a set of nearly two thousand genes that have failed to target even after several attempts. Recent attention has turned to the use of new genome editing technology for the generation of mutant alleles in mice. Here, we demonstrate how Cas9-assisted targeting can be combined with the IKMC targeting vector resource to generate conditional alleles in genes that have previously eluded targeting using conventional methods.
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spelling pubmed-50074772016-09-07 CRISPR-Cas9 enables conditional mutagenesis of challenging loci Schick, Joel A. Seisenberger, Claudia Beig, Joachim Bürger, Antje Iyer, Vivek Maier, Viola Perera, Sajith Rosen, Barry Skarnes, William C. Wurst, Wolfgang Sci Rep Article The International Knockout Mouse Consortium (IKMC) has produced a genome-wide collection of 15,000 isogenic targeting vectors for conditional mutagenesis in C57BL/6N mice. Although most of the vectors have been used successfully in murine embryonic stem (ES) cells, there remain a set of nearly two thousand genes that have failed to target even after several attempts. Recent attention has turned to the use of new genome editing technology for the generation of mutant alleles in mice. Here, we demonstrate how Cas9-assisted targeting can be combined with the IKMC targeting vector resource to generate conditional alleles in genes that have previously eluded targeting using conventional methods. Nature Publishing Group 2016-09-01 /pmc/articles/PMC5007477/ /pubmed/27580957 http://dx.doi.org/10.1038/srep32326 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Schick, Joel A.
Seisenberger, Claudia
Beig, Joachim
Bürger, Antje
Iyer, Vivek
Maier, Viola
Perera, Sajith
Rosen, Barry
Skarnes, William C.
Wurst, Wolfgang
CRISPR-Cas9 enables conditional mutagenesis of challenging loci
title CRISPR-Cas9 enables conditional mutagenesis of challenging loci
title_full CRISPR-Cas9 enables conditional mutagenesis of challenging loci
title_fullStr CRISPR-Cas9 enables conditional mutagenesis of challenging loci
title_full_unstemmed CRISPR-Cas9 enables conditional mutagenesis of challenging loci
title_short CRISPR-Cas9 enables conditional mutagenesis of challenging loci
title_sort crispr-cas9 enables conditional mutagenesis of challenging loci
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5007477/
https://www.ncbi.nlm.nih.gov/pubmed/27580957
http://dx.doi.org/10.1038/srep32326
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