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Mapping the nicking efficiencies of nickase R.BbvCI for side-specific LNA-substituted substrates using rolling circle amplification

We used a novel asymmetric cleavage analysis method based on rolling circle amplification (RCA) to determine the effects of LNA modification of substrate on the two subunits of R.BbvCI cleavage. We designed two sets of cleavage circular substrates by using two different ligation strategies and analy...

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Detalles Bibliográficos
Autores principales:	Wei, Hua, Zhao, Guojie, Hu, Tianyu, Tang, Suming, Jiang, Jiquan, Hu, Bo, Guan, Yifu
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group 2016
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5007493/ https://www.ncbi.nlm.nih.gov/pubmed/27582033 http://dx.doi.org/10.1038/srep32560

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