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A novel denitrifying methanotroph of the NC10 phylum and its microcolony

The NC10 phylum is a candidate phylum of prokaryotes and is considered important in biogeochemical cycles and evolutionary history. NC10 members are as-yet-uncultured and are difficult to enrich, and our knowledge regarding this phylum is largely limited to the first species ‘Candidatus Methylomirab...

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Autores principales: He, Zhanfei, Cai, Chaoyang, Wang, Jiaqi, Xu, Xinhua, Zheng, Ping, Jetten, Mike S. M., Hu, Baolan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5007514/
https://www.ncbi.nlm.nih.gov/pubmed/27582299
http://dx.doi.org/10.1038/srep32241
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author He, Zhanfei
Cai, Chaoyang
Wang, Jiaqi
Xu, Xinhua
Zheng, Ping
Jetten, Mike S. M.
Hu, Baolan
author_facet He, Zhanfei
Cai, Chaoyang
Wang, Jiaqi
Xu, Xinhua
Zheng, Ping
Jetten, Mike S. M.
Hu, Baolan
author_sort He, Zhanfei
collection PubMed
description The NC10 phylum is a candidate phylum of prokaryotes and is considered important in biogeochemical cycles and evolutionary history. NC10 members are as-yet-uncultured and are difficult to enrich, and our knowledge regarding this phylum is largely limited to the first species ‘Candidatus Methylomirabilis oxyfera’ (M. oxyfera). Here, we enriched NC10 members from paddy soil and obtained a novel species of the NC10 phylum that mediates the anaerobic oxidation of methane (AOM) coupled to nitrite reduction. By comparing the new 16S rRNA gene sequences with those already in the database, this new species was found to be widely distributed in various habitats in China. Therefore, we tentatively named it ‘Candidatus Methylomirabilis sinica’ (M. sinica). Cells of M. sinica are roughly coccus-shaped (0.7–1.2 μm), distinct from M. oxyfera (rod-shaped; 0.25–0.5 × 0.8–1.1 μm). Notably, microscopic inspections revealed that M. sinica grew in honeycomb-shaped microcolonies, which was the first discovery of microcolony of the NC10 phylum. This finding opens the possibility to isolate NC10 members using microcolony-dependent isolation strategies.
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spelling pubmed-50075142016-09-08 A novel denitrifying methanotroph of the NC10 phylum and its microcolony He, Zhanfei Cai, Chaoyang Wang, Jiaqi Xu, Xinhua Zheng, Ping Jetten, Mike S. M. Hu, Baolan Sci Rep Article The NC10 phylum is a candidate phylum of prokaryotes and is considered important in biogeochemical cycles and evolutionary history. NC10 members are as-yet-uncultured and are difficult to enrich, and our knowledge regarding this phylum is largely limited to the first species ‘Candidatus Methylomirabilis oxyfera’ (M. oxyfera). Here, we enriched NC10 members from paddy soil and obtained a novel species of the NC10 phylum that mediates the anaerobic oxidation of methane (AOM) coupled to nitrite reduction. By comparing the new 16S rRNA gene sequences with those already in the database, this new species was found to be widely distributed in various habitats in China. Therefore, we tentatively named it ‘Candidatus Methylomirabilis sinica’ (M. sinica). Cells of M. sinica are roughly coccus-shaped (0.7–1.2 μm), distinct from M. oxyfera (rod-shaped; 0.25–0.5 × 0.8–1.1 μm). Notably, microscopic inspections revealed that M. sinica grew in honeycomb-shaped microcolonies, which was the first discovery of microcolony of the NC10 phylum. This finding opens the possibility to isolate NC10 members using microcolony-dependent isolation strategies. Nature Publishing Group 2016-09-01 /pmc/articles/PMC5007514/ /pubmed/27582299 http://dx.doi.org/10.1038/srep32241 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
He, Zhanfei
Cai, Chaoyang
Wang, Jiaqi
Xu, Xinhua
Zheng, Ping
Jetten, Mike S. M.
Hu, Baolan
A novel denitrifying methanotroph of the NC10 phylum and its microcolony
title A novel denitrifying methanotroph of the NC10 phylum and its microcolony
title_full A novel denitrifying methanotroph of the NC10 phylum and its microcolony
title_fullStr A novel denitrifying methanotroph of the NC10 phylum and its microcolony
title_full_unstemmed A novel denitrifying methanotroph of the NC10 phylum and its microcolony
title_short A novel denitrifying methanotroph of the NC10 phylum and its microcolony
title_sort novel denitrifying methanotroph of the nc10 phylum and its microcolony
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5007514/
https://www.ncbi.nlm.nih.gov/pubmed/27582299
http://dx.doi.org/10.1038/srep32241
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