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Novel Foxp3(−) IL-10(−) Regulatory T-cells Induced by B-Cells Alleviate Intestinal Inflammation in Vivo

Recent studies have revealed various Foxp3(−) regulatory T (Treg) cell subsets effectively protect mice from colitis. In the present study, we demonstrated that B cells induced a particular subset of regulatory T (Treg-of-B) cells, expressing programmed cell death 1 (PD-1), inducible costimulator (I...

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Autores principales: Shao, Tzu-Yu, Hsu, Ling-Hui, Chien, Chien-Hui, Chiang, Bor-Luen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5007537/
https://www.ncbi.nlm.nih.gov/pubmed/27581189
http://dx.doi.org/10.1038/srep32415
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author Shao, Tzu-Yu
Hsu, Ling-Hui
Chien, Chien-Hui
Chiang, Bor-Luen
author_facet Shao, Tzu-Yu
Hsu, Ling-Hui
Chien, Chien-Hui
Chiang, Bor-Luen
author_sort Shao, Tzu-Yu
collection PubMed
description Recent studies have revealed various Foxp3(−) regulatory T (Treg) cell subsets effectively protect mice from colitis. In the present study, we demonstrated that B cells induced a particular subset of regulatory T (Treg-of-B) cells, expressing programmed cell death 1 (PD-1), inducible costimulator (ICOS), lymphocyte-activation gene 3 (LAG3), glucocorticoid-induced tumor necrosis factor receptor (GITR), and OX-40, did not express Foxp3. Treg-of-B cells produced abundant levels of IL-10 and low levels of IL-4 and TGF-β. Adoptive transfer of Treg-of-B cells protected mice from CD4(+)CD45RB(hi) T-cell-induced colitis, including infiltration of leukocytes, depletion of goblet cells, epithelial hyperplasia, and inhibition of Th1 and Th17 cytokines. These features were similar to IL-10-producing type 1 regulatory T (Tr1) cells; however, IL-10-deficient Treg-of-B cells maintained their suppressive function in vitro as well as in vivo, while the regulation of Tr1 cells depended on IL-10. In conclusion, Treg-of-B cells protected against experimental colitis through an IL-10-independent mechanism. We reported a novel subpopulation of regulatory T cells was different from conventional Foxp3(+) Treg and IL-10-producing Tr1 cells.
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spelling pubmed-50075372016-09-08 Novel Foxp3(−) IL-10(−) Regulatory T-cells Induced by B-Cells Alleviate Intestinal Inflammation in Vivo Shao, Tzu-Yu Hsu, Ling-Hui Chien, Chien-Hui Chiang, Bor-Luen Sci Rep Article Recent studies have revealed various Foxp3(−) regulatory T (Treg) cell subsets effectively protect mice from colitis. In the present study, we demonstrated that B cells induced a particular subset of regulatory T (Treg-of-B) cells, expressing programmed cell death 1 (PD-1), inducible costimulator (ICOS), lymphocyte-activation gene 3 (LAG3), glucocorticoid-induced tumor necrosis factor receptor (GITR), and OX-40, did not express Foxp3. Treg-of-B cells produced abundant levels of IL-10 and low levels of IL-4 and TGF-β. Adoptive transfer of Treg-of-B cells protected mice from CD4(+)CD45RB(hi) T-cell-induced colitis, including infiltration of leukocytes, depletion of goblet cells, epithelial hyperplasia, and inhibition of Th1 and Th17 cytokines. These features were similar to IL-10-producing type 1 regulatory T (Tr1) cells; however, IL-10-deficient Treg-of-B cells maintained their suppressive function in vitro as well as in vivo, while the regulation of Tr1 cells depended on IL-10. In conclusion, Treg-of-B cells protected against experimental colitis through an IL-10-independent mechanism. We reported a novel subpopulation of regulatory T cells was different from conventional Foxp3(+) Treg and IL-10-producing Tr1 cells. Nature Publishing Group 2016-09-01 /pmc/articles/PMC5007537/ /pubmed/27581189 http://dx.doi.org/10.1038/srep32415 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Shao, Tzu-Yu
Hsu, Ling-Hui
Chien, Chien-Hui
Chiang, Bor-Luen
Novel Foxp3(−) IL-10(−) Regulatory T-cells Induced by B-Cells Alleviate Intestinal Inflammation in Vivo
title Novel Foxp3(−) IL-10(−) Regulatory T-cells Induced by B-Cells Alleviate Intestinal Inflammation in Vivo
title_full Novel Foxp3(−) IL-10(−) Regulatory T-cells Induced by B-Cells Alleviate Intestinal Inflammation in Vivo
title_fullStr Novel Foxp3(−) IL-10(−) Regulatory T-cells Induced by B-Cells Alleviate Intestinal Inflammation in Vivo
title_full_unstemmed Novel Foxp3(−) IL-10(−) Regulatory T-cells Induced by B-Cells Alleviate Intestinal Inflammation in Vivo
title_short Novel Foxp3(−) IL-10(−) Regulatory T-cells Induced by B-Cells Alleviate Intestinal Inflammation in Vivo
title_sort novel foxp3(−) il-10(−) regulatory t-cells induced by b-cells alleviate intestinal inflammation in vivo
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5007537/
https://www.ncbi.nlm.nih.gov/pubmed/27581189
http://dx.doi.org/10.1038/srep32415
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