Multi-site tumor sampling (MSTS) improves the performance of histological detection of intratumor heterogeneity in clear cell renal cell carcinoma (CCRCC)

Current standard-of-care tumor sampling protocols for CCRCC (and other cancers) are not efficient at detecting intratumoural heterogeneity (ITH). We have demonstrated in silico that an alternative protocol, multi-site tumor sampling (MSTS) based upon the divide and conquer (DAC) algorithm, can signi...

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Detalles Bibliográficos
Autores principales: Guarch, Rosa, Cortés, Jesús M., Lawrie, Charles H., López, José I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: F1000Research 2016
Materias:
Study Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5007747/
https://www.ncbi.nlm.nih.gov/pubmed/27635226
http://dx.doi.org/10.12688/f1000research.9419.2
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author Guarch, Rosa
Cortés, Jesús M.
Lawrie, Charles H.
López, José I.
author_facet Guarch, Rosa
Cortés, Jesús M.
Lawrie, Charles H.
López, José I.
author_sort Guarch, Rosa
collection PubMed
description Current standard-of-care tumor sampling protocols for CCRCC (and other cancers) are not efficient at detecting intratumoural heterogeneity (ITH). We have demonstrated in silico that an alternative protocol, multi-site tumor sampling (MSTS) based upon the divide and conquer (DAC) algorithm, can significantly increase the efficiency of ITH detection without extra costs. Now we test this protocol on routine hematoxylin-eosin (HE) sections in a series of 38 CCRCC cases. MSTS was found to outperform traditional sampling when detecting either high grade (p=0.0136) or granular/eosinophilic cells (p=0.0114). We therefore propose that MSTS should be used in routine clinical practice.
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spelling pubmed-50077472016-09-14 Multi-site tumor sampling (MSTS) improves the performance of histological detection of intratumor heterogeneity in clear cell renal cell carcinoma (CCRCC) Guarch, Rosa Cortés, Jesús M. Lawrie, Charles H. López, José I. F1000Res Study Protocol Current standard-of-care tumor sampling protocols for CCRCC (and other cancers) are not efficient at detecting intratumoural heterogeneity (ITH). We have demonstrated in silico that an alternative protocol, multi-site tumor sampling (MSTS) based upon the divide and conquer (DAC) algorithm, can significantly increase the efficiency of ITH detection without extra costs. Now we test this protocol on routine hematoxylin-eosin (HE) sections in a series of 38 CCRCC cases. MSTS was found to outperform traditional sampling when detecting either high grade (p=0.0136) or granular/eosinophilic cells (p=0.0114). We therefore propose that MSTS should be used in routine clinical practice. F1000Research 2016-09-16 /pmc/articles/PMC5007747/ /pubmed/27635226 http://dx.doi.org/10.12688/f1000research.9419.2 Text en Copyright: © 2016 Guarch R et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Study Protocol
Guarch, Rosa
Cortés, Jesús M.
Lawrie, Charles H.
López, José I.
Multi-site tumor sampling (MSTS) improves the performance of histological detection of intratumor heterogeneity in clear cell renal cell carcinoma (CCRCC)
title Multi-site tumor sampling (MSTS) improves the performance of histological detection of intratumor heterogeneity in clear cell renal cell carcinoma (CCRCC)
title_full Multi-site tumor sampling (MSTS) improves the performance of histological detection of intratumor heterogeneity in clear cell renal cell carcinoma (CCRCC)
title_fullStr Multi-site tumor sampling (MSTS) improves the performance of histological detection of intratumor heterogeneity in clear cell renal cell carcinoma (CCRCC)
title_full_unstemmed Multi-site tumor sampling (MSTS) improves the performance of histological detection of intratumor heterogeneity in clear cell renal cell carcinoma (CCRCC)
title_short Multi-site tumor sampling (MSTS) improves the performance of histological detection of intratumor heterogeneity in clear cell renal cell carcinoma (CCRCC)
title_sort multi-site tumor sampling (msts) improves the performance of histological detection of intratumor heterogeneity in clear cell renal cell carcinoma (ccrcc)
topic Study Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5007747/
https://www.ncbi.nlm.nih.gov/pubmed/27635226
http://dx.doi.org/10.12688/f1000research.9419.2
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