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Influence of primer & probe chemistry and amplification target on reverse transcription digital PCR quantification of viral RNA

Compared to other PCR technologies, digital PCR is a potentially highly accurate approach for the quantification of nucleic acid fragments. This study describes the impact of four experimental factors, namely primer and probe chemistry, PCR amplification target, duplexing, and template type, on the...

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Detalles Bibliográficos
Autores principales:	Van Heuverswyn, Fran, Karczmarczyk, Maria, Schimmel, Heinz, Trapmann, Stefanie, Emons, Hendrik
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2016
Materias:	Research Paper
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5007883/ https://www.ncbi.nlm.nih.gov/pubmed/27617229 http://dx.doi.org/10.1016/j.bdq.2016.08.003

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