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Tertiary lymphoid structures are confined to patients presenting with unifocal Langerhans Cell Histiocytosis

Langerhans cell histiocytosis (LCH) is a neoplastic myeloid disorder with a thus far poorly understood immune component. Tertiary lymphoid structures (TLS) are lymph node-like entities which create an immune-promoting microenvironment at tumor sites. We analyzed the presence and clinical relevance o...

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Autores principales: Quispel, Willemijn T., Steenwijk, Eline C., van Unen, Vincent, Santos, Susy J., Koens, Lianne, Mebius, Reina, Egeler, R. Maarten, van Halteren, Astrid G. S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5007962/
https://www.ncbi.nlm.nih.gov/pubmed/27622056
http://dx.doi.org/10.1080/2162402X.2016.1164364
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author Quispel, Willemijn T.
Steenwijk, Eline C.
van Unen, Vincent
Santos, Susy J.
Koens, Lianne
Mebius, Reina
Egeler, R. Maarten
van Halteren, Astrid G. S.
author_facet Quispel, Willemijn T.
Steenwijk, Eline C.
van Unen, Vincent
Santos, Susy J.
Koens, Lianne
Mebius, Reina
Egeler, R. Maarten
van Halteren, Astrid G. S.
author_sort Quispel, Willemijn T.
collection PubMed
description Langerhans cell histiocytosis (LCH) is a neoplastic myeloid disorder with a thus far poorly understood immune component. Tertiary lymphoid structures (TLS) are lymph node-like entities which create an immune-promoting microenvironment at tumor sites. We analyzed the presence and clinical relevance of TLS in n = 104 H&E-stained, therapy-naive LCH lesions of non-lymphoid origin and applied immunohistochemistry to a smaller series. Lymphoid-follicular aggregates were detected in 34/104 (33%) lesions. In line with the lymphocyte recruitment capacity of MECA-79(+) high endothelial venules (HEVs), MECA-79(+)-expressing-LCH lesions (37/77, 48%) contained the most CD3(+) T-lymphocytes (p = 0.003). TLS were identified in 8/15 lesions and contained T-and B-lymphocytes, Follicular Dendritic Cells (FDC), HEVs and the chemokines CXCL13 and CCL21 representing key cellular components and TLS-inducing factors in conventional lymph nodes (LN). Lymphoid-follicular aggregates were most frequently detected in patients presenting with unifocal LCH (24/70, 34%) as compared to patients with poly-ostotic or multi-system LCH (7/30, 23%, p = 0.03). In addition, patients with lymphoid-follicular aggregates-containing lesions had the lowest risk to develop new LCH lesions (p = 0.04). The identification of various stages of TLS formation within LCH lesions may indicate a key role for the immune system in controlling aberrant histiocytes which arise in peripheral tissues.
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spelling pubmed-50079622017-02-23 Tertiary lymphoid structures are confined to patients presenting with unifocal Langerhans Cell Histiocytosis Quispel, Willemijn T. Steenwijk, Eline C. van Unen, Vincent Santos, Susy J. Koens, Lianne Mebius, Reina Egeler, R. Maarten van Halteren, Astrid G. S. Oncoimmunology Brief Report Langerhans cell histiocytosis (LCH) is a neoplastic myeloid disorder with a thus far poorly understood immune component. Tertiary lymphoid structures (TLS) are lymph node-like entities which create an immune-promoting microenvironment at tumor sites. We analyzed the presence and clinical relevance of TLS in n = 104 H&E-stained, therapy-naive LCH lesions of non-lymphoid origin and applied immunohistochemistry to a smaller series. Lymphoid-follicular aggregates were detected in 34/104 (33%) lesions. In line with the lymphocyte recruitment capacity of MECA-79(+) high endothelial venules (HEVs), MECA-79(+)-expressing-LCH lesions (37/77, 48%) contained the most CD3(+) T-lymphocytes (p = 0.003). TLS were identified in 8/15 lesions and contained T-and B-lymphocytes, Follicular Dendritic Cells (FDC), HEVs and the chemokines CXCL13 and CCL21 representing key cellular components and TLS-inducing factors in conventional lymph nodes (LN). Lymphoid-follicular aggregates were most frequently detected in patients presenting with unifocal LCH (24/70, 34%) as compared to patients with poly-ostotic or multi-system LCH (7/30, 23%, p = 0.03). In addition, patients with lymphoid-follicular aggregates-containing lesions had the lowest risk to develop new LCH lesions (p = 0.04). The identification of various stages of TLS formation within LCH lesions may indicate a key role for the immune system in controlling aberrant histiocytes which arise in peripheral tissues. Taylor & Francis 2016-03-28 /pmc/articles/PMC5007962/ /pubmed/27622056 http://dx.doi.org/10.1080/2162402X.2016.1164364 Text en © 2016 The Author(s). Published with license by Taylor & Francis Group, LLC http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License http://creativecommons.org/licenses/by-nc/3.0/, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.
spellingShingle Brief Report
Quispel, Willemijn T.
Steenwijk, Eline C.
van Unen, Vincent
Santos, Susy J.
Koens, Lianne
Mebius, Reina
Egeler, R. Maarten
van Halteren, Astrid G. S.
Tertiary lymphoid structures are confined to patients presenting with unifocal Langerhans Cell Histiocytosis
title Tertiary lymphoid structures are confined to patients presenting with unifocal Langerhans Cell Histiocytosis
title_full Tertiary lymphoid structures are confined to patients presenting with unifocal Langerhans Cell Histiocytosis
title_fullStr Tertiary lymphoid structures are confined to patients presenting with unifocal Langerhans Cell Histiocytosis
title_full_unstemmed Tertiary lymphoid structures are confined to patients presenting with unifocal Langerhans Cell Histiocytosis
title_short Tertiary lymphoid structures are confined to patients presenting with unifocal Langerhans Cell Histiocytosis
title_sort tertiary lymphoid structures are confined to patients presenting with unifocal langerhans cell histiocytosis
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5007962/
https://www.ncbi.nlm.nih.gov/pubmed/27622056
http://dx.doi.org/10.1080/2162402X.2016.1164364
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