Cargando…
Membrane Vesicles of Group B Streptococcus Disrupt Feto-Maternal Barrier Leading to Preterm Birth
Infection of the genitourinary tract with Group B Streptococcus (GBS), an opportunistic gram positive pathogen, is associated with premature rupture of amniotic membrane and preterm birth. In this work, we demonstrate that GBS produces membrane vesicles (MVs) in a serotype independent manner. These...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5008812/ https://www.ncbi.nlm.nih.gov/pubmed/27583406 http://dx.doi.org/10.1371/journal.ppat.1005816 |
_version_ | 1782451445180661760 |
---|---|
author | Surve, Manalee Vishnu Anil, Anjali Kamath, Kshama Ganesh Bhutda, Smita Sthanam, Lakshmi Kavitha Pradhan, Arpan Srivastava, Rohit Basu, Bhakti Dutta, Suryendu Sen, Shamik Modi, Deepak Banerjee, Anirban |
author_facet | Surve, Manalee Vishnu Anil, Anjali Kamath, Kshama Ganesh Bhutda, Smita Sthanam, Lakshmi Kavitha Pradhan, Arpan Srivastava, Rohit Basu, Bhakti Dutta, Suryendu Sen, Shamik Modi, Deepak Banerjee, Anirban |
author_sort | Surve, Manalee Vishnu |
collection | PubMed |
description | Infection of the genitourinary tract with Group B Streptococcus (GBS), an opportunistic gram positive pathogen, is associated with premature rupture of amniotic membrane and preterm birth. In this work, we demonstrate that GBS produces membrane vesicles (MVs) in a serotype independent manner. These MVs are loaded with virulence factors including extracellular matrix degrading proteases and pore forming toxins. Mice chorio-decidual membranes challenged with MVs ex vivo resulted in extensive collagen degradation leading to loss of stiffness and mechanical weakening. MVs when instilled vaginally are capable of anterograde transport in mouse reproductive tract. Intra-amniotic injections of GBS MVs in mice led to upregulation of pro-inflammatory cytokines and inflammation mimicking features of chorio-amnionitis; it also led to apoptosis in the chorio-decidual tissue. Instillation of MVs in the amniotic sac also resulted in intrauterine fetal death and preterm delivery. Our findings suggest that GBS MVs can independently orchestrate events at the feto-maternal interface causing chorio-amnionitis and membrane damage leading to preterm birth or fetal death. |
format | Online Article Text |
id | pubmed-5008812 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-50088122016-09-27 Membrane Vesicles of Group B Streptococcus Disrupt Feto-Maternal Barrier Leading to Preterm Birth Surve, Manalee Vishnu Anil, Anjali Kamath, Kshama Ganesh Bhutda, Smita Sthanam, Lakshmi Kavitha Pradhan, Arpan Srivastava, Rohit Basu, Bhakti Dutta, Suryendu Sen, Shamik Modi, Deepak Banerjee, Anirban PLoS Pathog Research Article Infection of the genitourinary tract with Group B Streptococcus (GBS), an opportunistic gram positive pathogen, is associated with premature rupture of amniotic membrane and preterm birth. In this work, we demonstrate that GBS produces membrane vesicles (MVs) in a serotype independent manner. These MVs are loaded with virulence factors including extracellular matrix degrading proteases and pore forming toxins. Mice chorio-decidual membranes challenged with MVs ex vivo resulted in extensive collagen degradation leading to loss of stiffness and mechanical weakening. MVs when instilled vaginally are capable of anterograde transport in mouse reproductive tract. Intra-amniotic injections of GBS MVs in mice led to upregulation of pro-inflammatory cytokines and inflammation mimicking features of chorio-amnionitis; it also led to apoptosis in the chorio-decidual tissue. Instillation of MVs in the amniotic sac also resulted in intrauterine fetal death and preterm delivery. Our findings suggest that GBS MVs can independently orchestrate events at the feto-maternal interface causing chorio-amnionitis and membrane damage leading to preterm birth or fetal death. Public Library of Science 2016-09-01 /pmc/articles/PMC5008812/ /pubmed/27583406 http://dx.doi.org/10.1371/journal.ppat.1005816 Text en © 2016 Surve et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Surve, Manalee Vishnu Anil, Anjali Kamath, Kshama Ganesh Bhutda, Smita Sthanam, Lakshmi Kavitha Pradhan, Arpan Srivastava, Rohit Basu, Bhakti Dutta, Suryendu Sen, Shamik Modi, Deepak Banerjee, Anirban Membrane Vesicles of Group B Streptococcus Disrupt Feto-Maternal Barrier Leading to Preterm Birth |
title | Membrane Vesicles of Group B Streptococcus Disrupt Feto-Maternal Barrier Leading to Preterm Birth |
title_full | Membrane Vesicles of Group B Streptococcus Disrupt Feto-Maternal Barrier Leading to Preterm Birth |
title_fullStr | Membrane Vesicles of Group B Streptococcus Disrupt Feto-Maternal Barrier Leading to Preterm Birth |
title_full_unstemmed | Membrane Vesicles of Group B Streptococcus Disrupt Feto-Maternal Barrier Leading to Preterm Birth |
title_short | Membrane Vesicles of Group B Streptococcus Disrupt Feto-Maternal Barrier Leading to Preterm Birth |
title_sort | membrane vesicles of group b streptococcus disrupt feto-maternal barrier leading to preterm birth |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5008812/ https://www.ncbi.nlm.nih.gov/pubmed/27583406 http://dx.doi.org/10.1371/journal.ppat.1005816 |
work_keys_str_mv | AT survemanaleevishnu membranevesiclesofgroupbstreptococcusdisruptfetomaternalbarrierleadingtopretermbirth AT anilanjali membranevesiclesofgroupbstreptococcusdisruptfetomaternalbarrierleadingtopretermbirth AT kamathkshamaganesh membranevesiclesofgroupbstreptococcusdisruptfetomaternalbarrierleadingtopretermbirth AT bhutdasmita membranevesiclesofgroupbstreptococcusdisruptfetomaternalbarrierleadingtopretermbirth AT sthanamlakshmikavitha membranevesiclesofgroupbstreptococcusdisruptfetomaternalbarrierleadingtopretermbirth AT pradhanarpan membranevesiclesofgroupbstreptococcusdisruptfetomaternalbarrierleadingtopretermbirth AT srivastavarohit membranevesiclesofgroupbstreptococcusdisruptfetomaternalbarrierleadingtopretermbirth AT basubhakti membranevesiclesofgroupbstreptococcusdisruptfetomaternalbarrierleadingtopretermbirth AT duttasuryendu membranevesiclesofgroupbstreptococcusdisruptfetomaternalbarrierleadingtopretermbirth AT senshamik membranevesiclesofgroupbstreptococcusdisruptfetomaternalbarrierleadingtopretermbirth AT modideepak membranevesiclesofgroupbstreptococcusdisruptfetomaternalbarrierleadingtopretermbirth AT banerjeeanirban membranevesiclesofgroupbstreptococcusdisruptfetomaternalbarrierleadingtopretermbirth |