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Efficient Production of Gene-Modified Mice using Staphylococcus aureus Cas9

The CRISPR/Cas system is an efficient genome-editing tool to modify genes in mouse zygotes. However, only the Streptococcus pyogenes Cas9 (SpCas9) has been systematically tested for generating gene-modified mice. The protospacer adjacent motif (PAM, 5′-NGG-3′) recognized by SpCas9 limits the number...

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Detalles Bibliográficos
Autores principales: Zhang, Xiya, Liang, Puping, Ding, Chenhui, Zhang, Zhen, Zhou, Jianwen, Xie, Xiaowei, Huang, Rui, Sun, Ying, Sun, Hongwei, Zhang, Jinran, Xu, Yanwen, Songyang, Zhou, Huang, Junjiu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5009317/
https://www.ncbi.nlm.nih.gov/pubmed/27586692
http://dx.doi.org/10.1038/srep32565

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