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Noninvasive mapping of the redox status of dimethylnitrosamine-induced hepatic fibrosis using in vivo dynamic nuclear polarization-magnetic resonance imaging
Hepatic fibrosis is a chronic disorder caused by viral infection and/or metabolic, genetic and cholestatic disorders. A noninvasive procedure that enables the detection of liver fibrosis based on redox status would be useful for disease identification and monitoring, and the development of treatment...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5009327/ https://www.ncbi.nlm.nih.gov/pubmed/27587186 http://dx.doi.org/10.1038/srep32604 |
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author | Kawano, Takahito Murata, Masaharu Hyodo, Fuminori Eto, Hinako Kosem, Nuttavut Nakata, Ryosuke Hamano, Nobuhito Piao, Jing Shu Narahara, Sayoko Akahoshi, Tomohiko Hashizume, Makoto |
author_facet | Kawano, Takahito Murata, Masaharu Hyodo, Fuminori Eto, Hinako Kosem, Nuttavut Nakata, Ryosuke Hamano, Nobuhito Piao, Jing Shu Narahara, Sayoko Akahoshi, Tomohiko Hashizume, Makoto |
author_sort | Kawano, Takahito |
collection | PubMed |
description | Hepatic fibrosis is a chronic disorder caused by viral infection and/or metabolic, genetic and cholestatic disorders. A noninvasive procedure that enables the detection of liver fibrosis based on redox status would be useful for disease identification and monitoring, and the development of treatments. However, an appropriate technique has not been reported. This study describes a novel method for assessing the redox status of the liver using in vivo dynamic nuclear polarization-magnetic resonance imaging (DNP-MRI) with the nitroxyl radical carbamoyl-PROXYL as a molecular imaging probe, which was tested in dimethylnitrosamine-treated mice as a model of liver fibrosis. Based on the pharmacokinetics of carbamoyl-PROXYL in control livers, reduction rate mapping was performed in fibrotic livers. Reduction rate maps demonstrated a clear difference between the redox status of control and fibrotic livers according to the expression of antioxidants. These findings indicate that in vivo DNP-MRI with a nitroxyl radical probe enables noninvasive detection of changes in liver redox status. |
format | Online Article Text |
id | pubmed-5009327 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-50093272016-09-08 Noninvasive mapping of the redox status of dimethylnitrosamine-induced hepatic fibrosis using in vivo dynamic nuclear polarization-magnetic resonance imaging Kawano, Takahito Murata, Masaharu Hyodo, Fuminori Eto, Hinako Kosem, Nuttavut Nakata, Ryosuke Hamano, Nobuhito Piao, Jing Shu Narahara, Sayoko Akahoshi, Tomohiko Hashizume, Makoto Sci Rep Article Hepatic fibrosis is a chronic disorder caused by viral infection and/or metabolic, genetic and cholestatic disorders. A noninvasive procedure that enables the detection of liver fibrosis based on redox status would be useful for disease identification and monitoring, and the development of treatments. However, an appropriate technique has not been reported. This study describes a novel method for assessing the redox status of the liver using in vivo dynamic nuclear polarization-magnetic resonance imaging (DNP-MRI) with the nitroxyl radical carbamoyl-PROXYL as a molecular imaging probe, which was tested in dimethylnitrosamine-treated mice as a model of liver fibrosis. Based on the pharmacokinetics of carbamoyl-PROXYL in control livers, reduction rate mapping was performed in fibrotic livers. Reduction rate maps demonstrated a clear difference between the redox status of control and fibrotic livers according to the expression of antioxidants. These findings indicate that in vivo DNP-MRI with a nitroxyl radical probe enables noninvasive detection of changes in liver redox status. Nature Publishing Group 2016-09-02 /pmc/articles/PMC5009327/ /pubmed/27587186 http://dx.doi.org/10.1038/srep32604 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Kawano, Takahito Murata, Masaharu Hyodo, Fuminori Eto, Hinako Kosem, Nuttavut Nakata, Ryosuke Hamano, Nobuhito Piao, Jing Shu Narahara, Sayoko Akahoshi, Tomohiko Hashizume, Makoto Noninvasive mapping of the redox status of dimethylnitrosamine-induced hepatic fibrosis using in vivo dynamic nuclear polarization-magnetic resonance imaging |
title | Noninvasive mapping of the redox status of dimethylnitrosamine-induced hepatic fibrosis using in vivo dynamic nuclear polarization-magnetic resonance imaging |
title_full | Noninvasive mapping of the redox status of dimethylnitrosamine-induced hepatic fibrosis using in vivo dynamic nuclear polarization-magnetic resonance imaging |
title_fullStr | Noninvasive mapping of the redox status of dimethylnitrosamine-induced hepatic fibrosis using in vivo dynamic nuclear polarization-magnetic resonance imaging |
title_full_unstemmed | Noninvasive mapping of the redox status of dimethylnitrosamine-induced hepatic fibrosis using in vivo dynamic nuclear polarization-magnetic resonance imaging |
title_short | Noninvasive mapping of the redox status of dimethylnitrosamine-induced hepatic fibrosis using in vivo dynamic nuclear polarization-magnetic resonance imaging |
title_sort | noninvasive mapping of the redox status of dimethylnitrosamine-induced hepatic fibrosis using in vivo dynamic nuclear polarization-magnetic resonance imaging |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5009327/ https://www.ncbi.nlm.nih.gov/pubmed/27587186 http://dx.doi.org/10.1038/srep32604 |
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