Cargando…

Noninvasive mapping of the redox status of dimethylnitrosamine-induced hepatic fibrosis using in vivo dynamic nuclear polarization-magnetic resonance imaging

Hepatic fibrosis is a chronic disorder caused by viral infection and/or metabolic, genetic and cholestatic disorders. A noninvasive procedure that enables the detection of liver fibrosis based on redox status would be useful for disease identification and monitoring, and the development of treatment...

Descripción completa

Detalles Bibliográficos
Autores principales: Kawano, Takahito, Murata, Masaharu, Hyodo, Fuminori, Eto, Hinako, Kosem, Nuttavut, Nakata, Ryosuke, Hamano, Nobuhito, Piao, Jing Shu, Narahara, Sayoko, Akahoshi, Tomohiko, Hashizume, Makoto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5009327/
https://www.ncbi.nlm.nih.gov/pubmed/27587186
http://dx.doi.org/10.1038/srep32604
_version_ 1782451505182277632
author Kawano, Takahito
Murata, Masaharu
Hyodo, Fuminori
Eto, Hinako
Kosem, Nuttavut
Nakata, Ryosuke
Hamano, Nobuhito
Piao, Jing Shu
Narahara, Sayoko
Akahoshi, Tomohiko
Hashizume, Makoto
author_facet Kawano, Takahito
Murata, Masaharu
Hyodo, Fuminori
Eto, Hinako
Kosem, Nuttavut
Nakata, Ryosuke
Hamano, Nobuhito
Piao, Jing Shu
Narahara, Sayoko
Akahoshi, Tomohiko
Hashizume, Makoto
author_sort Kawano, Takahito
collection PubMed
description Hepatic fibrosis is a chronic disorder caused by viral infection and/or metabolic, genetic and cholestatic disorders. A noninvasive procedure that enables the detection of liver fibrosis based on redox status would be useful for disease identification and monitoring, and the development of treatments. However, an appropriate technique has not been reported. This study describes a novel method for assessing the redox status of the liver using in vivo dynamic nuclear polarization-magnetic resonance imaging (DNP-MRI) with the nitroxyl radical carbamoyl-PROXYL as a molecular imaging probe, which was tested in dimethylnitrosamine-treated mice as a model of liver fibrosis. Based on the pharmacokinetics of carbamoyl-PROXYL in control livers, reduction rate mapping was performed in fibrotic livers. Reduction rate maps demonstrated a clear difference between the redox status of control and fibrotic livers according to the expression of antioxidants. These findings indicate that in vivo DNP-MRI with a nitroxyl radical probe enables noninvasive detection of changes in liver redox status.
format Online
Article
Text
id pubmed-5009327
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-50093272016-09-08 Noninvasive mapping of the redox status of dimethylnitrosamine-induced hepatic fibrosis using in vivo dynamic nuclear polarization-magnetic resonance imaging Kawano, Takahito Murata, Masaharu Hyodo, Fuminori Eto, Hinako Kosem, Nuttavut Nakata, Ryosuke Hamano, Nobuhito Piao, Jing Shu Narahara, Sayoko Akahoshi, Tomohiko Hashizume, Makoto Sci Rep Article Hepatic fibrosis is a chronic disorder caused by viral infection and/or metabolic, genetic and cholestatic disorders. A noninvasive procedure that enables the detection of liver fibrosis based on redox status would be useful for disease identification and monitoring, and the development of treatments. However, an appropriate technique has not been reported. This study describes a novel method for assessing the redox status of the liver using in vivo dynamic nuclear polarization-magnetic resonance imaging (DNP-MRI) with the nitroxyl radical carbamoyl-PROXYL as a molecular imaging probe, which was tested in dimethylnitrosamine-treated mice as a model of liver fibrosis. Based on the pharmacokinetics of carbamoyl-PROXYL in control livers, reduction rate mapping was performed in fibrotic livers. Reduction rate maps demonstrated a clear difference between the redox status of control and fibrotic livers according to the expression of antioxidants. These findings indicate that in vivo DNP-MRI with a nitroxyl radical probe enables noninvasive detection of changes in liver redox status. Nature Publishing Group 2016-09-02 /pmc/articles/PMC5009327/ /pubmed/27587186 http://dx.doi.org/10.1038/srep32604 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Kawano, Takahito
Murata, Masaharu
Hyodo, Fuminori
Eto, Hinako
Kosem, Nuttavut
Nakata, Ryosuke
Hamano, Nobuhito
Piao, Jing Shu
Narahara, Sayoko
Akahoshi, Tomohiko
Hashizume, Makoto
Noninvasive mapping of the redox status of dimethylnitrosamine-induced hepatic fibrosis using in vivo dynamic nuclear polarization-magnetic resonance imaging
title Noninvasive mapping of the redox status of dimethylnitrosamine-induced hepatic fibrosis using in vivo dynamic nuclear polarization-magnetic resonance imaging
title_full Noninvasive mapping of the redox status of dimethylnitrosamine-induced hepatic fibrosis using in vivo dynamic nuclear polarization-magnetic resonance imaging
title_fullStr Noninvasive mapping of the redox status of dimethylnitrosamine-induced hepatic fibrosis using in vivo dynamic nuclear polarization-magnetic resonance imaging
title_full_unstemmed Noninvasive mapping of the redox status of dimethylnitrosamine-induced hepatic fibrosis using in vivo dynamic nuclear polarization-magnetic resonance imaging
title_short Noninvasive mapping of the redox status of dimethylnitrosamine-induced hepatic fibrosis using in vivo dynamic nuclear polarization-magnetic resonance imaging
title_sort noninvasive mapping of the redox status of dimethylnitrosamine-induced hepatic fibrosis using in vivo dynamic nuclear polarization-magnetic resonance imaging
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5009327/
https://www.ncbi.nlm.nih.gov/pubmed/27587186
http://dx.doi.org/10.1038/srep32604
work_keys_str_mv AT kawanotakahito noninvasivemappingoftheredoxstatusofdimethylnitrosamineinducedhepaticfibrosisusinginvivodynamicnuclearpolarizationmagneticresonanceimaging
AT muratamasaharu noninvasivemappingoftheredoxstatusofdimethylnitrosamineinducedhepaticfibrosisusinginvivodynamicnuclearpolarizationmagneticresonanceimaging
AT hyodofuminori noninvasivemappingoftheredoxstatusofdimethylnitrosamineinducedhepaticfibrosisusinginvivodynamicnuclearpolarizationmagneticresonanceimaging
AT etohinako noninvasivemappingoftheredoxstatusofdimethylnitrosamineinducedhepaticfibrosisusinginvivodynamicnuclearpolarizationmagneticresonanceimaging
AT kosemnuttavut noninvasivemappingoftheredoxstatusofdimethylnitrosamineinducedhepaticfibrosisusinginvivodynamicnuclearpolarizationmagneticresonanceimaging
AT nakataryosuke noninvasivemappingoftheredoxstatusofdimethylnitrosamineinducedhepaticfibrosisusinginvivodynamicnuclearpolarizationmagneticresonanceimaging
AT hamanonobuhito noninvasivemappingoftheredoxstatusofdimethylnitrosamineinducedhepaticfibrosisusinginvivodynamicnuclearpolarizationmagneticresonanceimaging
AT piaojingshu noninvasivemappingoftheredoxstatusofdimethylnitrosamineinducedhepaticfibrosisusinginvivodynamicnuclearpolarizationmagneticresonanceimaging
AT naraharasayoko noninvasivemappingoftheredoxstatusofdimethylnitrosamineinducedhepaticfibrosisusinginvivodynamicnuclearpolarizationmagneticresonanceimaging
AT akahoshitomohiko noninvasivemappingoftheredoxstatusofdimethylnitrosamineinducedhepaticfibrosisusinginvivodynamicnuclearpolarizationmagneticresonanceimaging
AT hashizumemakoto noninvasivemappingoftheredoxstatusofdimethylnitrosamineinducedhepaticfibrosisusinginvivodynamicnuclearpolarizationmagneticresonanceimaging