Theophylline controllable RNAi-based genetic switches regulate expression of lncRNA TINCR and malignant phenotypes in bladder cancer cells

TINCR is a well-known lncRNA which acts as a master regulator in somatic differentiation development. However, it is still unclear whether TINCR is also involved in caner occurrence and progression. In this study, we observed that TINCR was up-regulated in bladder cancer tissues and cells and contri...

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Autores principales: Chen, Zhicong, Liu, Yuchen, He, Anbang, Li, Jianfa, Chen, Mingwei, Zhan, Yonghao, Lin, Junhao, Zhuang, Chengle, Liu, Li, Zhao, Guoping, Huang, Weiren, Cai, Zhiming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5009373/
https://www.ncbi.nlm.nih.gov/pubmed/27586866
http://dx.doi.org/10.1038/srep30798
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author Chen, Zhicong
Liu, Yuchen
He, Anbang
Li, Jianfa
Chen, Mingwei
Zhan, Yonghao
Lin, Junhao
Zhuang, Chengle
Liu, Li
Zhao, Guoping
Huang, Weiren
Cai, Zhiming
author_facet Chen, Zhicong
Liu, Yuchen
He, Anbang
Li, Jianfa
Chen, Mingwei
Zhan, Yonghao
Lin, Junhao
Zhuang, Chengle
Liu, Li
Zhao, Guoping
Huang, Weiren
Cai, Zhiming
author_sort Chen, Zhicong
collection PubMed
description TINCR is a well-known lncRNA which acts as a master regulator in somatic differentiation development. However, it is still unclear whether TINCR is also involved in caner occurrence and progression. In this study, we observed that TINCR was up-regulated in bladder cancer tissues and cells and contributed to oncogenesis and cancer progression. Silencing TINCR expression inhibited cell proliferation and promoted apoptosis in vitro, indicating that TINCR may be the potential therapeutic target for treating bladder urothelial carcinoma. Thus we used the synthetic biology approach to create theophylline controllable RNAi-based genetic switches which silenced TINCR in a dosage-dependent manner. Both RNAi-OFF and ON switches can be used to quantitatively control the expression of TINCR in bladder cancer to suppress the progression of bladder cancer. These findings suggest that lncRNA-TINCR could promote bladder cancer development and progression and artificial control of its expression through inducible RNAi may represent a new kind of therapeutic strategy for treating human bladder cancer.
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spelling pubmed-50093732016-09-12 Theophylline controllable RNAi-based genetic switches regulate expression of lncRNA TINCR and malignant phenotypes in bladder cancer cells Chen, Zhicong Liu, Yuchen He, Anbang Li, Jianfa Chen, Mingwei Zhan, Yonghao Lin, Junhao Zhuang, Chengle Liu, Li Zhao, Guoping Huang, Weiren Cai, Zhiming Sci Rep Article TINCR is a well-known lncRNA which acts as a master regulator in somatic differentiation development. However, it is still unclear whether TINCR is also involved in caner occurrence and progression. In this study, we observed that TINCR was up-regulated in bladder cancer tissues and cells and contributed to oncogenesis and cancer progression. Silencing TINCR expression inhibited cell proliferation and promoted apoptosis in vitro, indicating that TINCR may be the potential therapeutic target for treating bladder urothelial carcinoma. Thus we used the synthetic biology approach to create theophylline controllable RNAi-based genetic switches which silenced TINCR in a dosage-dependent manner. Both RNAi-OFF and ON switches can be used to quantitatively control the expression of TINCR in bladder cancer to suppress the progression of bladder cancer. These findings suggest that lncRNA-TINCR could promote bladder cancer development and progression and artificial control of its expression through inducible RNAi may represent a new kind of therapeutic strategy for treating human bladder cancer. Nature Publishing Group 2016-09-02 /pmc/articles/PMC5009373/ /pubmed/27586866 http://dx.doi.org/10.1038/srep30798 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Chen, Zhicong
Liu, Yuchen
He, Anbang
Li, Jianfa
Chen, Mingwei
Zhan, Yonghao
Lin, Junhao
Zhuang, Chengle
Liu, Li
Zhao, Guoping
Huang, Weiren
Cai, Zhiming
Theophylline controllable RNAi-based genetic switches regulate expression of lncRNA TINCR and malignant phenotypes in bladder cancer cells
title Theophylline controllable RNAi-based genetic switches regulate expression of lncRNA TINCR and malignant phenotypes in bladder cancer cells
title_full Theophylline controllable RNAi-based genetic switches regulate expression of lncRNA TINCR and malignant phenotypes in bladder cancer cells
title_fullStr Theophylline controllable RNAi-based genetic switches regulate expression of lncRNA TINCR and malignant phenotypes in bladder cancer cells
title_full_unstemmed Theophylline controllable RNAi-based genetic switches regulate expression of lncRNA TINCR and malignant phenotypes in bladder cancer cells
title_short Theophylline controllable RNAi-based genetic switches regulate expression of lncRNA TINCR and malignant phenotypes in bladder cancer cells
title_sort theophylline controllable rnai-based genetic switches regulate expression of lncrna tincr and malignant phenotypes in bladder cancer cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5009373/
https://www.ncbi.nlm.nih.gov/pubmed/27586866
http://dx.doi.org/10.1038/srep30798
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