Lentiviral expression system for the purification of secreted proteins from human cell cultures

BACKGROUND: Recombinant proteins of therapeutic use are ideally produced in human cells to ensure appropriate co- and post-translational modifications. However, purification of secreted proteins from the culture media is impeded by low expression from transfected cell lines and the presence of serum...

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Detalles Bibliográficos
Autores principales: Falkenhagen, Alexander, Asad, Sabah, Read, Stanley E., Joshi, Sadhna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5009704/
https://www.ncbi.nlm.nih.gov/pubmed/27590008
http://dx.doi.org/10.1186/s12896-016-0288-3

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5009704/
https://www.ncbi.nlm.nih.gov/pubmed/27590008
http://dx.doi.org/10.1186/s12896-016-0288-3

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