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Lentiviral expression system for the purification of secreted proteins from human cell cultures
BACKGROUND: Recombinant proteins of therapeutic use are ideally produced in human cells to ensure appropriate co- and post-translational modifications. However, purification of secreted proteins from the culture media is impeded by low expression from transfected cell lines and the presence of serum...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2016
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5009704/ https://www.ncbi.nlm.nih.gov/pubmed/27590008 http://dx.doi.org/10.1186/s12896-016-0288-3 |