Cargando…

Comparative proteome analysis of abdominal adipose tissues between fat and lean broilers

BACKGROUND: The molecular mechanism underlying broiler fat deposition is still poorly understood. METHOD: Currently, we used two-dimensional gel electrophoresis (2DE) to identify differentially expressed proteins in abdominal adipose tissues of birds at 4 week of age derived from Northeast Agricultu...

Descripción completa

Detalles Bibliográficos
Autores principales: Wu, Chun-Yan, Wu, Yuan-Yuan, Liu, Chun-Dong, Wang, Yu-Xiang, Na, Wei, Wang, Ning, Li, Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5009711/
https://www.ncbi.nlm.nih.gov/pubmed/27594807
http://dx.doi.org/10.1186/s12953-016-0100-2
_version_ 1782451566661337088
author Wu, Chun-Yan
Wu, Yuan-Yuan
Liu, Chun-Dong
Wang, Yu-Xiang
Na, Wei
Wang, Ning
Li, Hui
author_facet Wu, Chun-Yan
Wu, Yuan-Yuan
Liu, Chun-Dong
Wang, Yu-Xiang
Na, Wei
Wang, Ning
Li, Hui
author_sort Wu, Chun-Yan
collection PubMed
description BACKGROUND: The molecular mechanism underlying broiler fat deposition is still poorly understood. METHOD: Currently, we used two-dimensional gel electrophoresis (2DE) to identify differentially expressed proteins in abdominal adipose tissues of birds at 4 week of age derived from Northeast Agricultural University broiler lines divergently selected for abdominal fat content (NEAUHLF). RESULTS: Thirteen differentially expressed protein spots were screened out and identified by matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The protein spots were matched to thirteen proteins by searching against the NCBInr database. These identified proteins were apolipoprotein A-I (Apo A-I), cytokeratin otokeratin, ATP synthase subunit alpha, peptidyl-prolyl cis-trans isomerase FKBP4 (PPIase FKBP4), aspartate aminotransferase, carbonic anhydrase II (CA-II), prostaglandin-H2 D-isomerase precursor, fibrinogen alpha chain, lamin-A (LMNA), superoxide dismutase [Mn] (MnSOD), heat shock protein beta-1 (HSPβ1) and two predicted proteins. These differentially expressed proteins are involved mainly in lipid metabolism, amino acid metabolism, signal transduction, energy conversion, antioxidant, and cytoskeleton. Differential expression of Apo A-I, PPIase FKBP4, and cytokeratin otokeratin proteins were further confirmed by Western blot analysis. Quantitative real-time RT-PCR analyses showed that, of these 13 differentially expressed proteins, only PPIase FKBP4 and cytokeratin otokeratin were differentially expressed at mRNA level between the two lines. CONCLUSIONS: Our results have provided further information for understanding the basic genetics control of growth and development of broiler adipose tissue.
format Online
Article
Text
id pubmed-5009711
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-50097112016-09-03 Comparative proteome analysis of abdominal adipose tissues between fat and lean broilers Wu, Chun-Yan Wu, Yuan-Yuan Liu, Chun-Dong Wang, Yu-Xiang Na, Wei Wang, Ning Li, Hui Proteome Sci Research BACKGROUND: The molecular mechanism underlying broiler fat deposition is still poorly understood. METHOD: Currently, we used two-dimensional gel electrophoresis (2DE) to identify differentially expressed proteins in abdominal adipose tissues of birds at 4 week of age derived from Northeast Agricultural University broiler lines divergently selected for abdominal fat content (NEAUHLF). RESULTS: Thirteen differentially expressed protein spots were screened out and identified by matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The protein spots were matched to thirteen proteins by searching against the NCBInr database. These identified proteins were apolipoprotein A-I (Apo A-I), cytokeratin otokeratin, ATP synthase subunit alpha, peptidyl-prolyl cis-trans isomerase FKBP4 (PPIase FKBP4), aspartate aminotransferase, carbonic anhydrase II (CA-II), prostaglandin-H2 D-isomerase precursor, fibrinogen alpha chain, lamin-A (LMNA), superoxide dismutase [Mn] (MnSOD), heat shock protein beta-1 (HSPβ1) and two predicted proteins. These differentially expressed proteins are involved mainly in lipid metabolism, amino acid metabolism, signal transduction, energy conversion, antioxidant, and cytoskeleton. Differential expression of Apo A-I, PPIase FKBP4, and cytokeratin otokeratin proteins were further confirmed by Western blot analysis. Quantitative real-time RT-PCR analyses showed that, of these 13 differentially expressed proteins, only PPIase FKBP4 and cytokeratin otokeratin were differentially expressed at mRNA level between the two lines. CONCLUSIONS: Our results have provided further information for understanding the basic genetics control of growth and development of broiler adipose tissue. BioMed Central 2016-09-01 /pmc/articles/PMC5009711/ /pubmed/27594807 http://dx.doi.org/10.1186/s12953-016-0100-2 Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Wu, Chun-Yan
Wu, Yuan-Yuan
Liu, Chun-Dong
Wang, Yu-Xiang
Na, Wei
Wang, Ning
Li, Hui
Comparative proteome analysis of abdominal adipose tissues between fat and lean broilers
title Comparative proteome analysis of abdominal adipose tissues between fat and lean broilers
title_full Comparative proteome analysis of abdominal adipose tissues between fat and lean broilers
title_fullStr Comparative proteome analysis of abdominal adipose tissues between fat and lean broilers
title_full_unstemmed Comparative proteome analysis of abdominal adipose tissues between fat and lean broilers
title_short Comparative proteome analysis of abdominal adipose tissues between fat and lean broilers
title_sort comparative proteome analysis of abdominal adipose tissues between fat and lean broilers
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5009711/
https://www.ncbi.nlm.nih.gov/pubmed/27594807
http://dx.doi.org/10.1186/s12953-016-0100-2
work_keys_str_mv AT wuchunyan comparativeproteomeanalysisofabdominaladiposetissuesbetweenfatandleanbroilers
AT wuyuanyuan comparativeproteomeanalysisofabdominaladiposetissuesbetweenfatandleanbroilers
AT liuchundong comparativeproteomeanalysisofabdominaladiposetissuesbetweenfatandleanbroilers
AT wangyuxiang comparativeproteomeanalysisofabdominaladiposetissuesbetweenfatandleanbroilers
AT nawei comparativeproteomeanalysisofabdominaladiposetissuesbetweenfatandleanbroilers
AT wangning comparativeproteomeanalysisofabdominaladiposetissuesbetweenfatandleanbroilers
AT lihui comparativeproteomeanalysisofabdominaladiposetissuesbetweenfatandleanbroilers