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A Csf1r-EGFP Transgene Provides a Novel Marker for Monocyte Subsets in Sheep
Expression of Csf1r in adults is restricted to cells of the macrophage lineage. Transgenic reporters based upon the Csf1r locus require inclusion of the highly conserved Fms-intronic regulatory element for expression. We have created Csf1r-EGFP transgenic sheep via lentiviral transgenesis of a const...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
AAI
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5009875/ https://www.ncbi.nlm.nih.gov/pubmed/27521343 http://dx.doi.org/10.4049/jimmunol.1502336 |
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author | Pridans, Clare Davis, Gemma M. Sauter, Kristin A. Lisowski, Zofia M. Corripio-Miyar, Yolanda Raper, Anna Lefevre, Lucas Young, Rachel McCulloch, Mary E. Lillico, Simon Milne, Elspeth Whitelaw, Bruce Hume, David A. |
author_facet | Pridans, Clare Davis, Gemma M. Sauter, Kristin A. Lisowski, Zofia M. Corripio-Miyar, Yolanda Raper, Anna Lefevre, Lucas Young, Rachel McCulloch, Mary E. Lillico, Simon Milne, Elspeth Whitelaw, Bruce Hume, David A. |
author_sort | Pridans, Clare |
collection | PubMed |
description | Expression of Csf1r in adults is restricted to cells of the macrophage lineage. Transgenic reporters based upon the Csf1r locus require inclusion of the highly conserved Fms-intronic regulatory element for expression. We have created Csf1r-EGFP transgenic sheep via lentiviral transgenesis of a construct containing elements of the mouse Fms-intronic regulatory element and Csf1r promoter. Committed bone marrow macrophage precursors and blood monocytes express EGFP in these animals. Sheep monocytes were divided into three populations, similar to classical, intermediate, and nonclassical monocytes in humans, based upon CD14 and CD16 expression. All expressed EGFP, with increased levels in the nonclassical subset. Because Csf1r expression coincides with the earliest commitment to the macrophage lineage, Csf1r-EGFP bone marrow provides a tool for studying the earliest events in myelopoiesis using the sheep as a model. |
format | Online Article Text |
id | pubmed-5009875 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | AAI |
record_format | MEDLINE/PubMed |
spelling | pubmed-50098752016-09-06 A Csf1r-EGFP Transgene Provides a Novel Marker for Monocyte Subsets in Sheep Pridans, Clare Davis, Gemma M. Sauter, Kristin A. Lisowski, Zofia M. Corripio-Miyar, Yolanda Raper, Anna Lefevre, Lucas Young, Rachel McCulloch, Mary E. Lillico, Simon Milne, Elspeth Whitelaw, Bruce Hume, David A. J Immunol Immune System Development Expression of Csf1r in adults is restricted to cells of the macrophage lineage. Transgenic reporters based upon the Csf1r locus require inclusion of the highly conserved Fms-intronic regulatory element for expression. We have created Csf1r-EGFP transgenic sheep via lentiviral transgenesis of a construct containing elements of the mouse Fms-intronic regulatory element and Csf1r promoter. Committed bone marrow macrophage precursors and blood monocytes express EGFP in these animals. Sheep monocytes were divided into three populations, similar to classical, intermediate, and nonclassical monocytes in humans, based upon CD14 and CD16 expression. All expressed EGFP, with increased levels in the nonclassical subset. Because Csf1r expression coincides with the earliest commitment to the macrophage lineage, Csf1r-EGFP bone marrow provides a tool for studying the earliest events in myelopoiesis using the sheep as a model. AAI 2016-09-15 2016-08-12 /pmc/articles/PMC5009875/ /pubmed/27521343 http://dx.doi.org/10.4049/jimmunol.1502336 Text en Copyright © 2016 The Authors This is an open-access article distributed under the terms of the CC-BY 3.0 Unported license. |
spellingShingle | Immune System Development Pridans, Clare Davis, Gemma M. Sauter, Kristin A. Lisowski, Zofia M. Corripio-Miyar, Yolanda Raper, Anna Lefevre, Lucas Young, Rachel McCulloch, Mary E. Lillico, Simon Milne, Elspeth Whitelaw, Bruce Hume, David A. A Csf1r-EGFP Transgene Provides a Novel Marker for Monocyte Subsets in Sheep |
title | A Csf1r-EGFP Transgene Provides a Novel Marker for Monocyte Subsets in Sheep |
title_full | A Csf1r-EGFP Transgene Provides a Novel Marker for Monocyte Subsets in Sheep |
title_fullStr | A Csf1r-EGFP Transgene Provides a Novel Marker for Monocyte Subsets in Sheep |
title_full_unstemmed | A Csf1r-EGFP Transgene Provides a Novel Marker for Monocyte Subsets in Sheep |
title_short | A Csf1r-EGFP Transgene Provides a Novel Marker for Monocyte Subsets in Sheep |
title_sort | csf1r-egfp transgene provides a novel marker for monocyte subsets in sheep |
topic | Immune System Development |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5009875/ https://www.ncbi.nlm.nih.gov/pubmed/27521343 http://dx.doi.org/10.4049/jimmunol.1502336 |
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