High-Quality Genome Assembly and Annotation for Plasmodium coatneyi, Generated Using Single-Molecule Real-Time PacBio Technology

Plasmodium coatneyi is a protozoan parasite species that causes simian malaria and is an excellent model for studying disease caused by the human malaria parasite, P. falciparum. Here we report the complete (nontelomeric) genome sequence of P. coatneyi Hackeri generated by the application of only Pa...

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Detalles Bibliográficos
Autores principales: Chien, Jung-Ting, Pakala, Suman B., Geraldo, Juliana A., Lapp, Stacey A., Humphrey, Jay C., Barnwell, John W., Kissinger, Jessica C., Galinski, Mary R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2016
Materias:
Eukaryotes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5009967/
https://www.ncbi.nlm.nih.gov/pubmed/27587810
http://dx.doi.org/10.1128/genomeA.00883-16
Descripción
Sumario:Plasmodium coatneyi is a protozoan parasite species that causes simian malaria and is an excellent model for studying disease caused by the human malaria parasite, P. falciparum. Here we report the complete (nontelomeric) genome sequence of P. coatneyi Hackeri generated by the application of only Pacific Biosciences RS II (PacBio RS II) single-molecule real-time (SMRT) high-resolution sequence technology and assembly using the Hierarchical Genome Assembly Process (HGAP). This is the first Plasmodium genome sequence reported to use only PacBio technology. This approach has proven to be superior to short-read only approaches for this species.

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