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Coordination of Cellular Dynamics Contributes to Tooth Epithelium Deformations

The morphologies of ectodermal organs are shaped by appropriate combinations of several deformation modes, such as invagination and anisotropic tissue elongation. However, how multicellular dynamics are coordinated during deformation processes remains to be elucidated. Here, we developed a four-dime...

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Autores principales: Morita, Ritsuko, Kihira, Miho, Nakatsu, Yousuke, Nomoto, Yohei, Ogawa, Miho, Ohashi, Kazumasa, Mizuno, Kensaku, Tachikawa, Tetsuhiko, Ishimoto, Yukitaka, Morishita, Yoshihiro, Tsuji, Takashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5010284/
https://www.ncbi.nlm.nih.gov/pubmed/27588418
http://dx.doi.org/10.1371/journal.pone.0161336
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author Morita, Ritsuko
Kihira, Miho
Nakatsu, Yousuke
Nomoto, Yohei
Ogawa, Miho
Ohashi, Kazumasa
Mizuno, Kensaku
Tachikawa, Tetsuhiko
Ishimoto, Yukitaka
Morishita, Yoshihiro
Tsuji, Takashi
author_facet Morita, Ritsuko
Kihira, Miho
Nakatsu, Yousuke
Nomoto, Yohei
Ogawa, Miho
Ohashi, Kazumasa
Mizuno, Kensaku
Tachikawa, Tetsuhiko
Ishimoto, Yukitaka
Morishita, Yoshihiro
Tsuji, Takashi
author_sort Morita, Ritsuko
collection PubMed
description The morphologies of ectodermal organs are shaped by appropriate combinations of several deformation modes, such as invagination and anisotropic tissue elongation. However, how multicellular dynamics are coordinated during deformation processes remains to be elucidated. Here, we developed a four-dimensional (4D) analysis system for tracking cell movement and division at a single-cell resolution in developing tooth epithelium. The expression patterns of a Fucci probe clarified the region- and stage-specific cell cycle patterns within the tooth germ, which were in good agreement with the pattern of the volume growth rate estimated from tissue-level deformation analysis. Cellular motility was higher in the regions with higher growth rates, while the mitotic orientation was significantly biased along the direction of tissue elongation in the epithelium. Further, these spatio-temporal patterns of cellular dynamics and tissue-level deformation were highly correlated with that of the activity of cofilin, which is an actin depolymerization factor, suggesting that the coordination of cellular dynamics via actin remodeling plays an important role in tooth epithelial morphogenesis. Our system enhances the understanding of how cellular behaviors are coordinated during ectodermal organogenesis, which cannot be observed from histological analyses.
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spelling pubmed-50102842016-09-27 Coordination of Cellular Dynamics Contributes to Tooth Epithelium Deformations Morita, Ritsuko Kihira, Miho Nakatsu, Yousuke Nomoto, Yohei Ogawa, Miho Ohashi, Kazumasa Mizuno, Kensaku Tachikawa, Tetsuhiko Ishimoto, Yukitaka Morishita, Yoshihiro Tsuji, Takashi PLoS One Research Article The morphologies of ectodermal organs are shaped by appropriate combinations of several deformation modes, such as invagination and anisotropic tissue elongation. However, how multicellular dynamics are coordinated during deformation processes remains to be elucidated. Here, we developed a four-dimensional (4D) analysis system for tracking cell movement and division at a single-cell resolution in developing tooth epithelium. The expression patterns of a Fucci probe clarified the region- and stage-specific cell cycle patterns within the tooth germ, which were in good agreement with the pattern of the volume growth rate estimated from tissue-level deformation analysis. Cellular motility was higher in the regions with higher growth rates, while the mitotic orientation was significantly biased along the direction of tissue elongation in the epithelium. Further, these spatio-temporal patterns of cellular dynamics and tissue-level deformation were highly correlated with that of the activity of cofilin, which is an actin depolymerization factor, suggesting that the coordination of cellular dynamics via actin remodeling plays an important role in tooth epithelial morphogenesis. Our system enhances the understanding of how cellular behaviors are coordinated during ectodermal organogenesis, which cannot be observed from histological analyses. Public Library of Science 2016-09-02 /pmc/articles/PMC5010284/ /pubmed/27588418 http://dx.doi.org/10.1371/journal.pone.0161336 Text en © 2016 Morita et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Morita, Ritsuko
Kihira, Miho
Nakatsu, Yousuke
Nomoto, Yohei
Ogawa, Miho
Ohashi, Kazumasa
Mizuno, Kensaku
Tachikawa, Tetsuhiko
Ishimoto, Yukitaka
Morishita, Yoshihiro
Tsuji, Takashi
Coordination of Cellular Dynamics Contributes to Tooth Epithelium Deformations
title Coordination of Cellular Dynamics Contributes to Tooth Epithelium Deformations
title_full Coordination of Cellular Dynamics Contributes to Tooth Epithelium Deformations
title_fullStr Coordination of Cellular Dynamics Contributes to Tooth Epithelium Deformations
title_full_unstemmed Coordination of Cellular Dynamics Contributes to Tooth Epithelium Deformations
title_short Coordination of Cellular Dynamics Contributes to Tooth Epithelium Deformations
title_sort coordination of cellular dynamics contributes to tooth epithelium deformations
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5010284/
https://www.ncbi.nlm.nih.gov/pubmed/27588418
http://dx.doi.org/10.1371/journal.pone.0161336
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