Novel Approach to Repeated Arterial Blood Sampling in Small Animal PET: Application in a Test-Retest Study with the Adenosine A1 Receptor Ligand [(11)C]MPDX

PURPOSE: Small animal positron emission tomography (PET) can be used to detect small changes in neuroreceptor availability. This often requires rapid arterial blood sampling. However, current catheterization procedures do not allow repeated blood sampling. We have developed a procedure which allows...

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Autores principales: Sijbesma, Jürgen W. A., Zhou, Xiaoyun, Vállez García, David, Houwertjes, Martin C., Doorduin, Janine, Kwizera, Chantal, Maas, Bram, Meerlo, Peter, Dierckx, Rudi A., Slart, Riemer H. J. A., Elsinga, Philip H., van Waarde, Aren
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5010612/
https://www.ncbi.nlm.nih.gov/pubmed/27091332
http://dx.doi.org/10.1007/s11307-016-0954-9
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author Sijbesma, Jürgen W. A.
Zhou, Xiaoyun
Vállez García, David
Houwertjes, Martin C.
Doorduin, Janine
Kwizera, Chantal
Maas, Bram
Meerlo, Peter
Dierckx, Rudi A.
Slart, Riemer H. J. A.
Elsinga, Philip H.
van Waarde, Aren
author_facet Sijbesma, Jürgen W. A.
Zhou, Xiaoyun
Vállez García, David
Houwertjes, Martin C.
Doorduin, Janine
Kwizera, Chantal
Maas, Bram
Meerlo, Peter
Dierckx, Rudi A.
Slart, Riemer H. J. A.
Elsinga, Philip H.
van Waarde, Aren
author_sort Sijbesma, Jürgen W. A.
collection PubMed
description PURPOSE: Small animal positron emission tomography (PET) can be used to detect small changes in neuroreceptor availability. This often requires rapid arterial blood sampling. However, current catheterization procedures do not allow repeated blood sampling. We have developed a procedure which allows arterial sampling on repeated occasions in the same animal. PROCEDURES: Eleven male Wistar rats were two times catheterized via a superficial branch of a femoral artery and scanned with [(11)C]MPDX and blood sampling. PET images were co-registered to a magnetic resonance imaging (MRI) template. Regional tracer distribution volumes (V(T)) in the brain were calculated by the Logan analysis. The procedure was repeated after 1 week. RESULTS: Surgery was successful in 90 % of the cases, and discomfort was minor. The V(T) data showed small differences between test and retest, low between subject variability, and a strong agreement between and within subjects. CONCLUSION: Repeated quantitative imaging with a high reproducibility is possible with this approach.
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spelling pubmed-50106122016-09-16 Novel Approach to Repeated Arterial Blood Sampling in Small Animal PET: Application in a Test-Retest Study with the Adenosine A1 Receptor Ligand [(11)C]MPDX Sijbesma, Jürgen W. A. Zhou, Xiaoyun Vállez García, David Houwertjes, Martin C. Doorduin, Janine Kwizera, Chantal Maas, Bram Meerlo, Peter Dierckx, Rudi A. Slart, Riemer H. J. A. Elsinga, Philip H. van Waarde, Aren Mol Imaging Biol Research Article PURPOSE: Small animal positron emission tomography (PET) can be used to detect small changes in neuroreceptor availability. This often requires rapid arterial blood sampling. However, current catheterization procedures do not allow repeated blood sampling. We have developed a procedure which allows arterial sampling on repeated occasions in the same animal. PROCEDURES: Eleven male Wistar rats were two times catheterized via a superficial branch of a femoral artery and scanned with [(11)C]MPDX and blood sampling. PET images were co-registered to a magnetic resonance imaging (MRI) template. Regional tracer distribution volumes (V(T)) in the brain were calculated by the Logan analysis. The procedure was repeated after 1 week. RESULTS: Surgery was successful in 90 % of the cases, and discomfort was minor. The V(T) data showed small differences between test and retest, low between subject variability, and a strong agreement between and within subjects. CONCLUSION: Repeated quantitative imaging with a high reproducibility is possible with this approach. Springer US 2016-04-18 2016 /pmc/articles/PMC5010612/ /pubmed/27091332 http://dx.doi.org/10.1007/s11307-016-0954-9 Text en © The Author(s) 2016 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Research Article
Sijbesma, Jürgen W. A.
Zhou, Xiaoyun
Vállez García, David
Houwertjes, Martin C.
Doorduin, Janine
Kwizera, Chantal
Maas, Bram
Meerlo, Peter
Dierckx, Rudi A.
Slart, Riemer H. J. A.
Elsinga, Philip H.
van Waarde, Aren
Novel Approach to Repeated Arterial Blood Sampling in Small Animal PET: Application in a Test-Retest Study with the Adenosine A1 Receptor Ligand [(11)C]MPDX
title Novel Approach to Repeated Arterial Blood Sampling in Small Animal PET: Application in a Test-Retest Study with the Adenosine A1 Receptor Ligand [(11)C]MPDX
title_full Novel Approach to Repeated Arterial Blood Sampling in Small Animal PET: Application in a Test-Retest Study with the Adenosine A1 Receptor Ligand [(11)C]MPDX
title_fullStr Novel Approach to Repeated Arterial Blood Sampling in Small Animal PET: Application in a Test-Retest Study with the Adenosine A1 Receptor Ligand [(11)C]MPDX
title_full_unstemmed Novel Approach to Repeated Arterial Blood Sampling in Small Animal PET: Application in a Test-Retest Study with the Adenosine A1 Receptor Ligand [(11)C]MPDX
title_short Novel Approach to Repeated Arterial Blood Sampling in Small Animal PET: Application in a Test-Retest Study with the Adenosine A1 Receptor Ligand [(11)C]MPDX
title_sort novel approach to repeated arterial blood sampling in small animal pet: application in a test-retest study with the adenosine a1 receptor ligand [(11)c]mpdx
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5010612/
https://www.ncbi.nlm.nih.gov/pubmed/27091332
http://dx.doi.org/10.1007/s11307-016-0954-9
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